PMID- 26892016 OWN - NLM STAT- MEDLINE DCOM- 20180109 LR - 20181202 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 1516 DP - 2016 TI - Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice. PG - 145-152 LID - 10.1007/7651_2015_315 [doi] AB - Stem cells are cells capable of proliferation, self-renewal, and differentiation into specific phenotypes. They are an essential part of tissue engineering, which is used in regenerative medicine in case of degenerative diseases. In this chapter, we describe the methods of isolating and culturing various types of stem cells, like human embryonic stem cells (hESCs), human umbilical cord derived mesenchymal stem cells (hUC-MSCs), murine bone marrow derived mesenchymal stem cells (mBM-MSCs), murine adipose tissue derived mesenchymal stem cells (mAD-MSCs), and murine bone marrow derived dendritic cells (mBMDCs). All these cell types can be used in tissue engineering techniques. FAU - Kar, Srabani AU - Kar S AD - Immunology and Regenerative Medicine Research Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India. FAU - Mitra, Shinjini AU - Mitra S AD - Immunology and Regenerative Medicine Research Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India. FAU - Banerjee, Ena Ray AU - Banerjee ER AD - Immunology and Regenerative Medicine Research Laboratory, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India. enarb1@gmail.com. AD - Department of Zoology, University of Calcutta, Kolkata, 700019, West Bengal, India. enarb1@gmail.com. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - Adipose Tissue/cytology MH - Animals MH - Cell Differentiation/genetics MH - Cell Proliferation/*genetics MH - Cell Separation/*methods MH - Dendritic Cells/cytology MH - Human Embryonic Stem Cells/*cytology MH - Humans MH - Mesenchymal Stem Cells/cytology MH - Mice MH - Mouse Embryonic Stem Cells/*cytology MH - Umbilical Cord/cytology OTO - NOTNLM OT - Adipose derived mesenchymal stem cells (AD-MSCs) OT - Bone marrow derived dendritic cells (BMDCs) OT - Bone marrow derived mesenchymal stem cells (BM-MSCs) OT - Embryoid body (EB) OT - Embryonic stem cells (ESCs) OT - Lineage specific differentiation OT - Mesenchymal stem cells (MSCs) OT - Stem cells OT - Umbilical cord (UC) OT - Undifferentiated cells EDAT- 2016/02/20 06:00 MHDA- 2018/01/10 06:00 CRDT- 2016/02/20 06:00 PHST- 2016/02/20 06:00 [entrez] PHST- 2016/02/20 06:00 [pubmed] PHST- 2018/01/10 06:00 [medline] AID - 10.1007/7651_2015_315 [doi] PST - ppublish SO - Methods Mol Biol. 2016;1516:145-152. doi: 10.1007/7651_2015_315.