PMID- 26893624
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 11
IP  - 2
DP  - 2016 Feb
TI  - Puerarin attenuates the inflammatory response and apoptosis in LPS-stimulated 
      cardiomyocytes.
PG  - 415-420
AB  - Patients with septic shock suffer from high mortality rates, particularly when 
      complicated by severe myocardial depression which is characterized by hypotension 
      and a reduction in cardiac output. Inflammation is an important factor involved 
      in the early stages of sepsis. The aim of the present study was to investigate 
      the effect of the Chinese herbal compound puerarin (1, 5, 10, 20 and 40 microM) on 
      cardiomyocyte inflammatory response in a sepsis model using H9c2 cardiomyocytes 
      stimulated with 1 microg/ml lipopolysaccharide (LPS). The mRNA expression levels of 
      tumor necrosis factor (TNF)-alpha and interleukin (IL)-beta were evaluated using reverse 
      transcription-quantitative polymerase chain reaction. In addition, the protein 
      expression levels of various factors were determined using western blot analysis. 
      Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling was used to 
      evaluate the apoptosis rates in the various groups, and immunocytochemical 
      analysis was employed to determine the effect of puerarin on the nuclear 
      translocation of p65 protein. The present study demonstrated that LPS stimulation 
      increased IL-1beta and TNF-alpha mRNA expression levels, as compared with the controls 
      (P<0.05). Following treatment with various concentrations of puerarin, the 
      expression levels of IL-1beta and TNF-alpha were markedly blunted, particularly in the 
      LPS + 40 microM puerarin group (P<0.05 vs. the LPS group). Furthermore, puerarin 
      administration significantly inhibited LPS-induced apoptosis in H9c2 
      cardiomyocytes, as determined by terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) staining (TUNEL positive cells: LPS + 40 microM 
      puerarin group, 5.5% vs. LPS group, 10.5%; P<0.01). In addition, puerarin 
      significantly decreased LPS-induced phosphorylated nuclear factor (p-NF)-kappaB p65 
      and Bax expression levels, and increased the expression levels of Bcl-2, as 
      compared with the LPS group (P<0.05). These data indicated that puerarin may 
      serve as a valuable protective agent against cardiovascular inflammatory 
      diseases.
FAU - Yuan, Yuan
AU  - Yuan Y
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei 430060, P.R. China.
FAU - Zhou, Heng
AU  - Zhou H
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei 430060, P.R. China.
FAU - Wu, Qing-Qing
AU  - Wu QQ
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei 430060, P.R. China.
FAU - Li, Fang-Fang
AU  - Li FF
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei 430060, P.R. China.
FAU - Bian, Zhou-Yan
AU  - Bian ZY
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei 430060, P.R. China.
FAU - Deng, Wei
AU  - Deng W
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei 430060, P.R. China.
FAU - Zhou, Meng-Qiao
AU  - Zhou MQ
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei 430060, P.R. China.
FAU - Tang, Qi-Zhu
AU  - Tang QZ
AD  - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China; Cardiovascular Research Institute of Wuhan University, Wuhan, 
      Hubei 430060, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20151204
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4734177
OTO - NOTNLM
OT  - apoptosis
OT  - inflammation
OT  - lipopolysaccharide
OT  - nuclear factor-kappaB
OT  - puerarin
EDAT- 2016/02/20 06:00
MHDA- 2016/02/20 06:01
PMCR- 2015/12/04
CRDT- 2016/02/20 06:00
PHST- 2014/09/19 00:00 [received]
PHST- 2015/10/26 00:00 [accepted]
PHST- 2016/02/20 06:00 [entrez]
PHST- 2016/02/20 06:00 [pubmed]
PHST- 2016/02/20 06:01 [medline]
PHST- 2015/12/04 00:00 [pmc-release]
AID - ETM-0-0-2910 [pii]
AID - 10.3892/etm.2015.2910 [doi]
PST - ppublish
SO  - Exp Ther Med. 2016 Feb;11(2):415-420. doi: 10.3892/etm.2015.2910. Epub 2015 Dec 
      4.