PMID- 26896301
OWN - NLM
STAT- MEDLINE
DCOM- 20170714
LR  - 20240109
IS  - 1467-7652 (Electronic)
IS  - 1467-7644 (Print)
IS  - 1467-7644 (Linking)
VI  - 14
IP  - 8
DP  - 2016 Aug
TI  - Bean pod mottle virus: a new powerful tool for functional genomics studies in 
      Pisum sativum.
PG  - 1777-87
LID - 10.1111/pbi.12537 [doi]
AB  - Pea (Pisum sativum L.) is an important legume worldwide. The importance of pea in 
      arable rotations and nutritional value for both human and animal consumption have 
      fostered sustained production and different studies to improve agronomic traits 
      of interest. Moreover, complete sequencing of the pea genome is currently 
      underway and will lead to the identification of a large number of genes 
      potentially associated with important agronomic traits. Because stable genetic 
      transformation is laborious for pea, virus-induced gene silencing (VIGS) appears 
      as a powerful alternative technology for determining the function of unknown 
      genes. In this work, we present a rapid and efficient viral inoculation method 
      using DNA infectious plasmids of Bean pod mottle virus (BPMV)-derived VIGS 
      vector. Six pea genotypes with important genes controlling biotic and/or abiotic 
      stresses were found susceptible to BPMV carrying a GFP reporter gene and showed 
      fluorescence in both shoots and roots. In a second step, we investigated 37 
      additional pea genotypes and found that 30 were susceptible to BPMV and only 7 
      were resistant. The capacity of BPMV to induce silencing of endogenes was 
      investigated in the most susceptible genotype using two visual reporter genes: 
      PsPDS and PsKORRIGAN1 (PsKOR1) encoding PHYTOENE DESATURASE and a 
      1,4-beta-D-glucanase, respectively. The features of the 'one-step' BPMV-derived VIGS 
      vector include (i) the ease of rub-inoculation, without any need for biolistic or 
      agro-inoculation procedures, (ii) simple cost-effective procedure and (iii) 
      noninterference of viral symptoms with silencing. These features make BPMV the 
      most adapted VIGS vector in pea to make low- to high-throughput VIGS studies.
CI  - (c) 2016 The Authors. Plant Biotechnology Journal published by Society for 
      Experimental Biology and The Association of Applied Biologists and John Wiley & 
      Sons Ltd.
FAU - Meziadi, Chouaib
AU  - Meziadi C
AD  - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite 
      Paris-Sud, Universite d'Evry, Universite Paris-Diderot Sorbonne Paris-Cite, 
      Universite Paris-Saclay, Orsay, France.
FAU - Blanchet, Sophie
AU  - Blanchet S
AD  - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite 
      Paris-Sud, Universite d'Evry, Universite Paris-Diderot Sorbonne Paris-Cite, 
      Universite Paris-Saclay, Orsay, France.
FAU - Richard, Manon M S
AU  - Richard MM
AD  - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite 
      Paris-Sud, Universite d'Evry, Universite Paris-Diderot Sorbonne Paris-Cite, 
      Universite Paris-Saclay, Orsay, France.
FAU - Pilet-Nayel, Marie-Laure
AU  - Pilet-Nayel ML
AD  - INRA, UMR 1349 IGEPP, Le Rheu, France.
FAU - Geffroy, Valerie
AU  - Geffroy V
AD  - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite 
      Paris-Sud, Universite d'Evry, Universite Paris-Diderot Sorbonne Paris-Cite, 
      Universite Paris-Saclay, Orsay, France.
FAU - Pflieger, Stephanie
AU  - Pflieger S
AD  - Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRA, Universite 
      Paris-Sud, Universite d'Evry, Universite Paris-Diderot Sorbonne Paris-Cite, 
      Universite Paris-Saclay, Orsay, France.
LA  - eng
SI  - GENBANK/AJ621355.1
SI  - GENBANK/AJ621573.1
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160220
PL  - England
TA  - Plant Biotechnol J
JT  - Plant biotechnology journal
JID - 101201889
RN  - 0 (Plant Proteins)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.99.- (phytoene dehydrogenase)
SB  - IM
MH  - Comovirus/*genetics/pathogenicity
MH  - Gene Silencing
MH  - Genetic Vectors
MH  - Genomics/*methods
MH  - Genotype
MH  - Oxidoreductases/genetics
MH  - Pisum sativum/*genetics/*virology
MH  - Plant Components, Aerial/virology
MH  - Plant Diseases/virology
MH  - Plant Proteins/genetics
MH  - Plant Roots/virology
PMC - PMC5067550
OTO - NOTNLM
OT  - Bean pod mottle virus
OT  - Pisum sativum
OT  - RNAi
OT  - functional validation
OT  - legume
OT  - post-transcriptional gene silencing
EDAT- 2016/02/21 06:00
MHDA- 2017/07/15 06:00
PMCR- 2016/10/18
CRDT- 2016/02/21 06:00
PHST- 2015/08/26 00:00 [received]
PHST- 2015/11/13 00:00 [revised]
PHST- 2016/01/06 00:00 [accepted]
PHST- 2016/02/21 06:00 [entrez]
PHST- 2016/02/21 06:00 [pubmed]
PHST- 2017/07/15 06:00 [medline]
PHST- 2016/10/18 00:00 [pmc-release]
AID - PBI12537 [pii]
AID - 10.1111/pbi.12537 [doi]
PST - ppublish
SO  - Plant Biotechnol J. 2016 Aug;14(8):1777-87. doi: 10.1111/pbi.12537. Epub 2016 Feb 
      20.