PMID- 26897848
OWN - NLM
STAT- MEDLINE
DCOM- 20160321
LR  - 20160222
IS  - 0047-1860 (Print)
IS  - 0047-1860 (Linking)
VI  - 63
IP  - 10
DP  - 2015 Oct
TI  - [Feasibility Study for Storage and Re-Utilization of Circulating Tumor Cells 
      (CTCs)].
PG  - 1137-43
AB  - BACKGROUND: In this era of precision medicine, monitoring patients requires not 
      only real time but also longitudinal sequence of samples at various time points. 
      Based on this background, we focused on conditioned circumstances on fixation and 
      storage for re-utilization of CTCs. MATERIALS: Instead of actual CTCs, Cell line 
      (H1975) derived from lung cancer was used because of their scarceness of CTCs. 
      METHODS: These cells were put on a slide by using an auto-smear device. The 
      slides were evaluated under various centrifuge forces, fixations for the 
      following storages. RESULTS AND DISCUSSION: The study indicated that 800 rpm for 
      1 min centrifuge and fixation by 95% ETOH was excellent. Further at least 5 cells 
      per 1 mL cell solution were required for the following procedures including 
      Fluorescence in situ hybridization (FISH) analysis. This study provides insights 
      of new platform for evaluation of CTCs not only real time but also longitudinal 
      sequence at various time points.
FAU - Kakishima, Hiroki
AU  - Kakishima H
FAU - Wakai, Susumu
AU  - Wakai S
FAU - Shibuki, Yasuo
AU  - Shibuki Y
FAU - Sasaki, Naoshi
AU  - Sasaki N
FAU - Taniguchi, Hirokazu
AU  - Taniguchi H
FAU - Tsuta, Koji
AU  - Tsuta K
FAU - Furuta, Koh
AU  - Furuta K
LA  - jpn
PT  - English Abstract
PT  - Journal Article
PL  - Japan
TA  - Rinsho Byori
JT  - Rinsho byori. The Japanese journal of clinical pathology
JID - 2984781R
SB  - IM
MH  - Cell Line, Tumor
MH  - Cytological Techniques/*methods
MH  - Feasibility Studies
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/pathology
MH  - Neoplastic Cells, Circulating/*pathology
MH  - Specimen Handling/*methods
EDAT- 2016/02/24 06:00
MHDA- 2016/03/22 06:00
CRDT- 2016/02/23 06:00
PHST- 2016/02/23 06:00 [entrez]
PHST- 2016/02/24 06:00 [pubmed]
PHST- 2016/03/22 06:00 [medline]
PST - ppublish
SO  - Rinsho Byori. 2015 Oct;63(10):1137-43.