PMID- 26898431
OWN - NLM
STAT- MEDLINE
DCOM- 20161114
LR  - 20181202
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 78
DP  - 2016 Mar
TI  - Overexpressing NKx2.5 increases the differentiation of human umbilical cord 
      drived mesenchymal stem cells into cardiomyocyte-like cells.
PG  - 110-115
LID - S0753-3322(15)30115-3 [pii]
LID - 10.1016/j.biopha.2016.01.020 [doi]
AB  - BACKGROUND: Nkx2.5 is one of the transcription factors in early myocardial cell 
      development and Nkx2.5 gene expression increased gradually in the course of stem 
      cells differentiation. In this study, this study aimed to investigate whether 
      overexpression of NKx2.5 increases human umbilical cord drived mesenchymal stem 
      cells (hUCMSCs) transdifferentiation into a cardiac phenotype in vitro. METHODS: 
      hUCMSCs were transduced with Nkx2.5 at the third passage (transduced group). Gene 
      expression of cTnI, Desmin, Nkx2.5 and GATA-4 in transduced group was analyzed 
      using real-time PCR and immunohistochemistry, and compared with no-transduced 
      hUCMSCs (control group), which were transfected with green fluorescent protein 
      (GFP) only. RESULTS: Compared with control group, hUCMSCs in transduced group 
      were shown by immunofluorescence to have higher expression of Nkx2.5. After 
      incubation for 4 weeks, the mRNA and protein expression of cardiac genes, 
      including cTnI, Desmin, Nkx2.5 and GATA-4, were up- regulated in transduced group 
      compared with control group (P<0.05). CONCLUSIONS: Overexpression of Nkx2.5 
      significantly promotes the differentiation of hUCMSCs into cardiomyocytes and 
      increases the expression of cTnI, Desmin, Nkx2.5, and GATA-4.
CI  - Copyright (c) 2016 Elsevier Masson SAS. All rights reserved.
FAU - Ruan, Zhongbao
AU  - Ruan Z
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou 225300, PR China. 
      Electronic address: tzcardiac@163.com.
FAU - Zhu, Li
AU  - Zhu L
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou 225300, PR China.
FAU - Yin, Yigang
AU  - Yin Y
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou 225300, PR China.
FAU - Chen, Gecai
AU  - Chen G
AD  - Department of Cardiology, Taizhou People's Hospital, Taizhou 225300, PR China.
LA  - eng
PT  - Journal Article
DEP - 20160124
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Biomarkers)
RN  - 0 (Desmin)
RN  - 0 (GATA4 Transcription Factor)
RN  - 0 (Homeobox Protein Nkx-2.5)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (NKX2-5 protein, human)
RN  - 0 (Transcription Factors)
RN  - 0 (Troponin I)
SB  - IM
MH  - Biomarkers/metabolism
MH  - *Cell Differentiation/genetics
MH  - Desmin/genetics/metabolism
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - GATA4 Transcription Factor/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Homeobox Protein Nkx-2.5
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Humans
MH  - Infant, Newborn
MH  - Mesenchymal Stem Cells/*cytology/*metabolism
MH  - Myocytes, Cardiac/*cytology/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Transcription Factors/genetics/*metabolism
MH  - Transfection
MH  - Troponin I/genetics/metabolism
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Cardiomyocyte-like cells
OT  - Human umbilical cord drived mesenchymal stem cells
OT  - Nkx2.5
OT  - Overexpression
EDAT- 2016/02/24 06:00
MHDA- 2016/11/15 06:00
CRDT- 2016/02/23 06:00
PHST- 2015/09/01 00:00 [received]
PHST- 2015/12/19 00:00 [revised]
PHST- 2016/01/13 00:00 [accepted]
PHST- 2016/02/23 06:00 [entrez]
PHST- 2016/02/24 06:00 [pubmed]
PHST- 2016/11/15 06:00 [medline]
AID - S0753-3322(15)30115-3 [pii]
AID - 10.1016/j.biopha.2016.01.020 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2016 Mar;78:110-115. doi: 10.1016/j.biopha.2016.01.020. Epub 
      2016 Jan 24.