PMID- 26899353
OWN - NLM
STAT- MEDLINE
DCOM- 20170203
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Feb 22
TI  - Graphene oxide-dependent growth and self-aggregation into a hydrogel complex of 
      exoelectrogenic bacteria.
PG  - 21867
LID - 10.1038/srep21867 [doi]
LID - 21867
AB  - Graphene oxide (GO) is reduced by certain exoelectrogenic bacteria, but its 
      effects on bacterial growth and metabolism are a controversial issue. This study 
      aimed to determine whether GO functions as the terminal electron acceptor to 
      allow specific growth of and electricity production by exoelectrogenic bacteria. 
      Cultivation of environmental samples with GO and acetate as the sole substrate 
      could specifically enrich exoelectrogenic bacteria with Geobacter species 
      predominating (51-68% of the total populations). Interestingly, bacteria in these 
      cultures self-aggregated into a conductive hydrogel complex together with 
      biologically reduced GO (rGO). A novel GO-respiring bacterium designated 
      Geobacter sp. strain R4 was isolated from this hydrogel complex. This organism 
      exhibited stable electricity production at >1000 muA/cm(3) (at 200 mV vs Ag/AgCl) 
      for more than 60 d via rGO while temporary electricity production using graphite 
      felt. The better electricity production depends upon the characteristics of rGO 
      such as a large surface area for biofilm growth, greater capacitance, and smaller 
      internal resistance. This is the first report to demonstrate GO-dependent growth 
      of exoelectrogenic bacteria while forming a conductive hydrogel complex with rGO. 
      The simple put-and-wait process leading to the formation of hydrogel complexes of 
      rGO and exoelectrogens will enable wider applications of GO to bioelectrochemical 
      systems.
FAU - Yoshida, Naoko
AU  - Yoshida N
AD  - Center for Fostering Young and Innovative Researchers, Nagoya Institute of 
      Technology, Nagoya, Aichi 466-8555, Japan.
AD  - Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi 
      University of Technology, Toyohashi, Aichi 441-8580, Japan.
FAU - Miyata, Yasushi
AU  - Miyata Y
AD  - Nagoya Municipal Industrial Research Institute, Nagoya, Aichi 456-0058, Japan.
FAU - Doi, Kasumi
AU  - Doi K
AD  - Department of Civil Engineering, Nagoya Institute of Technology, Nagoya, Aichi 
      466-8555, Japan.
FAU - Goto, Yuko
AU  - Goto Y
AD  - Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi 
      University of Technology, Toyohashi, Aichi 441-8580, Japan.
AD  - Department of Biomedical Science, College of Life and Health Science, Chubu 
      University, Kasugai, Aichi 487-8501, Japan.
FAU - Nagao, Yuji
AU  - Nagao Y
AD  - Department of Environmental and Life Sciences, Toyohashi University of 
      Technology, Toyohashi, Aichi 441-8580, Japan.
FAU - Tero, Ryugo
AU  - Tero R
AD  - Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi 
      University of Technology, Toyohashi, Aichi 441-8580, Japan.
AD  - Department of Environmental and Life Sciences, Toyohashi University of 
      Technology, Toyohashi, Aichi 441-8580, Japan.
FAU - Hiraishi, Akira
AU  - Hiraishi A
AD  - Electronics-Inspired Interdisciplinary Research Institute (EIIRIS), Toyohashi 
      University of Technology, Toyohashi, Aichi 441-8580, Japan.
AD  - Department of Environmental and Life Sciences, Toyohashi University of 
      Technology, Toyohashi, Aichi 441-8580, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160222
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Hydrogels)
RN  - 0 (Oxides)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Bioelectric Energy Sources
MH  - *Biofilms
MH  - Electric Capacitance
MH  - Geobacter/*growth & development/physiology/ultrastructure
MH  - Graphite/*chemistry
MH  - Hydrogels/*chemistry
MH  - Molecular Typing
MH  - Oxides/chemistry
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
PMC - PMC4761877
COIS- There is competing financial interest. We are currently applying for patents on 
      the methods reported in the paper.
EDAT- 2016/02/24 06:00
MHDA- 2017/02/06 06:00
PMCR- 2016/02/22
CRDT- 2016/02/23 06:00
PHST- 2015/10/09 00:00 [received]
PHST- 2016/02/02 00:00 [accepted]
PHST- 2016/02/23 06:00 [entrez]
PHST- 2016/02/24 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
PHST- 2016/02/22 00:00 [pmc-release]
AID - srep21867 [pii]
AID - 10.1038/srep21867 [doi]
PST - epublish
SO  - Sci Rep. 2016 Feb 22;6:21867. doi: 10.1038/srep21867.