PMID- 26900298
OWN - NLM
STAT- MEDLINE
DCOM- 20170117
LR  - 20181113
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 22
IP  - 7
DP  - 2016 Feb 21
TI  - Effects of endoplasmic reticulum stress on the expression of inflammatory 
      cytokines in patients with ulcerative colitis.
PG  - 2357-65
LID - 10.3748/wjg.v22.i7.2357 [doi]
AB  - AIM: To explore the changes of X-box binding protein 1 splicing (XBP1s) and 
      inflammatory cytokine expression in patients with ulcerative colitis (UC) in 
      response to endoplasmic reticulum stress (ERS). METHODS: Reverse transcription 
      polymerase chain reaction and quantitative polymerase chain reaction were 
      performed to detect the forms of XBP1s and the expression of interleukin (IL)-2, 
      interferon (IFN)-gamma, and IL-17alpha. Differences between patients with UC and normal 
      subjects were then determined. RESULTS: Mononuclear cells of the peripheral blood 
      of normal subjects and UC patients with were stimulated with no drugs (control), 
      phytohemagglutinin (PHA), thapsigargin (TG), or both PHA and TG. XBP1s in 
      patients with UC exhibited splicing, which was greater with co-stimulation than 
      single stimulation. Co-stimulation increased the expression level of IL-2, IFN-gamma, 
      and IL-17alpha. CONCLUSION: The T lymphocytes of both normal subjects and patients 
      with UC responded to ERS by activating the XBP1s-mediated signalling pathway, 
      upregulating the expression of inflammatory cytokines, and increasing the 
      occurrence of inflammation. The mononuclear cells in the peripheral blood of 
      patients with UC were more sensitive to ERS than those in the peripheral blood of 
      normal subjects.
FAU - Li, Nan
AU  - Li N
AD  - Nan Li, Xue-Ming Wang, Li-Jun Jiang, Meng Zhang, Na Li, Zhen-Zhen Wei, Nan Zheng, 
      Ya-Jiao Zhao, Department of Gastrology, PLA 309 Hospital, Beijing 100091, China.
FAU - Wang, Xue-Ming
AU  - Wang XM
AD  - Nan Li, Xue-Ming Wang, Li-Jun Jiang, Meng Zhang, Na Li, Zhen-Zhen Wei, Nan Zheng, 
      Ya-Jiao Zhao, Department of Gastrology, PLA 309 Hospital, Beijing 100091, China.
FAU - Jiang, Li-Jun
AU  - Jiang LJ
AD  - Nan Li, Xue-Ming Wang, Li-Jun Jiang, Meng Zhang, Na Li, Zhen-Zhen Wei, Nan Zheng, 
      Ya-Jiao Zhao, Department of Gastrology, PLA 309 Hospital, Beijing 100091, China.
FAU - Zhang, Meng
AU  - Zhang M
AD  - Nan Li, Xue-Ming Wang, Li-Jun Jiang, Meng Zhang, Na Li, Zhen-Zhen Wei, Nan Zheng, 
      Ya-Jiao Zhao, Department of Gastrology, PLA 309 Hospital, Beijing 100091, China.
FAU - Li, Na
AU  - Li N
AD  - Nan Li, Xue-Ming Wang, Li-Jun Jiang, Meng Zhang, Na Li, Zhen-Zhen Wei, Nan Zheng, 
      Ya-Jiao Zhao, Department of Gastrology, PLA 309 Hospital, Beijing 100091, China.
FAU - Wei, Zhen-Zhen
AU  - Wei ZZ
AD  - Nan Li, Xue-Ming Wang, Li-Jun Jiang, Meng Zhang, Na Li, Zhen-Zhen Wei, Nan Zheng, 
      Ya-Jiao Zhao, Department of Gastrology, PLA 309 Hospital, Beijing 100091, China.
FAU - Zheng, Nan
AU  - Zheng N
AD  - Nan Li, Xue-Ming Wang, Li-Jun Jiang, Meng Zhang, Na Li, Zhen-Zhen Wei, Nan Zheng, 
      Ya-Jiao Zhao, Department of Gastrology, PLA 309 Hospital, Beijing 100091, China.
FAU - Zhao, Ya-Jiao
AU  - Zhao YJ
AD  - Nan Li, Xue-Ming Wang, Li-Jun Jiang, Meng Zhang, Na Li, Zhen-Zhen Wei, Nan Zheng, 
      Ya-Jiao Zhao, Department of Gastrology, PLA 309 Hospital, Beijing 100091, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Cytokines)
RN  - 0 (IFNG protein, human)
RN  - 0 (IL17A protein, human)
RN  - 0 (IL2 protein, human)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-2)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (X-Box Binding Protein 1)
RN  - 0 (XBP1 protein, human)
RN  - 67526-95-8 (Thapsigargin)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Colitis, Ulcerative/blood/genetics/immunology/*metabolism
MH  - Cytokines/genetics/immunology/*metabolism
MH  - *Endoplasmic Reticulum Stress/drug effects
MH  - Female
MH  - Humans
MH  - Inflammation Mediators/immunology/*metabolism
MH  - Interferon-gamma/genetics/metabolism
MH  - Interleukin-17/genetics/metabolism
MH  - Interleukin-2/genetics/metabolism
MH  - Lymphocyte Activation
MH  - Male
MH  - Middle Aged
MH  - Phytohemagglutinins/pharmacology
MH  - Signal Transduction
MH  - T-Lymphocytes/drug effects/immunology/*metabolism
MH  - Thapsigargin/pharmacology
MH  - Up-Regulation
MH  - X-Box Binding Protein 1/genetics/metabolism
MH  - Young Adult
PMC - PMC4735010
OTO - NOTNLM
OT  - Endoplasmic reticulum stress
OT  - Ulcerative colitis
OT  - X-box binding protein 1 splicing
EDAT- 2016/02/24 06:00
MHDA- 2017/01/18 06:00
PMCR- 2016/02/21
CRDT- 2016/02/23 06:00
PHST- 2015/05/24 00:00 [received]
PHST- 2015/09/05 00:00 [revised]
PHST- 2015/11/30 00:00 [accepted]
PHST- 2016/02/23 06:00 [entrez]
PHST- 2016/02/24 06:00 [pubmed]
PHST- 2017/01/18 06:00 [medline]
PHST- 2016/02/21 00:00 [pmc-release]
AID - 10.3748/wjg.v22.i7.2357 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2016 Feb 21;22(7):2357-65. doi: 10.3748/wjg.v22.i7.2357.