PMID- 26900946
OWN - NLM
STAT- MEDLINE
DCOM- 20160720
LR  - 20240327
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 2
DP  - 2016
TI  - An Oxalyl-CoA Dependent Pathway of Oxalate Catabolism Plays a Role in Regulating 
      Calcium Oxalate Crystal Accumulation and Defending against Oxalate-Secreting 
      Phytopathogens in Medicago truncatula.
PG  - e0149850
LID - 10.1371/journal.pone.0149850 [doi]
LID - e0149850
AB  - Considering the widespread occurrence of oxalate in nature and its broad impact 
      on a host of organisms, it is surprising that so little is known about the 
      turnover of this important acid. In plants, oxalate oxidase is the most well 
      studied enzyme capable of degrading oxalate, but not all plants possess this 
      activity. Recently, an Acyl Activating Enzyme 3 (AAE3), encoding an oxalyl-CoA 
      synthetase, was identified in Arabidopsis. AAE3 has been proposed to catalyze the 
      first step in an alternative pathway of oxalate degradation. Whether this enzyme 
      and proposed pathway is important to other plants is unknown. Here, we identify 
      the Medicago truncatula AAE3 (MtAAE3) and show that it encodes an oxalyl-CoA 
      synthetase activity exhibiting high activity against oxalate with a Km = 81 +/- 9 
      muM and Vmax = 19 +/- 0.9 mumoles min-1mg protein-1. GFP-MtAAE3 localization 
      suggested that this enzyme functions within the cytosol of the cell. Mtaae3 
      knock-down line showed a reduction in its ability to degrade oxalate into CO2. 
      This reduction in the capacity to degrade oxalate resulted in the accumulation of 
      druse crystals of calcium oxalate in the Mtaae3 knock-down line and an increased 
      susceptibility to oxalate-secreting phytopathogens such as Sclerotinia 
      sclerotiorum. Taken together, these results suggest that AAE3 dependent turnover 
      of oxalate is important to different plants and functions in the regulation of 
      tissue calcium oxalate crystal accumulation and in defense against 
      oxalate-secreting phytopathogens.
FAU - Foster, Justin
AU  - Foster J
AD  - USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor 
      College of Medicine, Houston, Texas, 77030-2600, United States of America.
FAU - Luo, Bin
AU  - Luo B
AD  - USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor 
      College of Medicine, Houston, Texas, 77030-2600, United States of America.
FAU - Nakata, Paul A
AU  - Nakata PA
AD  - USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor 
      College of Medicine, Houston, Texas, 77030-2600, United States of America.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160222
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Acyl Coenzyme A)
RN  - 0 (oxalyl-coenzyme A)
RN  - 2612HC57YE (Calcium Oxalate)
SB  - IM
MH  - Acyl Coenzyme A/*metabolism
MH  - Ascomycota/*physiology
MH  - Calcium Oxalate/*metabolism
MH  - Medicago truncatula/*metabolism/*microbiology
MH  - Plant Diseases/microbiology
PMC - PMC4763187
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/02/24 06:00
MHDA- 2016/07/21 06:00
PMCR- 2016/02/22
CRDT- 2016/02/23 06:00
PHST- 2015/12/08 00:00 [received]
PHST- 2016/02/05 00:00 [accepted]
PHST- 2016/02/23 06:00 [entrez]
PHST- 2016/02/24 06:00 [pubmed]
PHST- 2016/07/21 06:00 [medline]
PHST- 2016/02/22 00:00 [pmc-release]
AID - PONE-D-15-53323 [pii]
AID - 10.1371/journal.pone.0149850 [doi]
PST - epublish
SO  - PLoS One. 2016 Feb 22;11(2):e0149850. doi: 10.1371/journal.pone.0149850. 
      eCollection 2016.