PMID- 26902085
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160223
LR  - 20200930
IS  - 1976-9148 (Print)
IS  - 2005-4483 (Electronic)
IS  - 1976-9148 (Linking)
VI  - 24
IP  - 2
DP  - 2016 Mar 1
TI  - Apigenin Regulates Interleukin-1beta-Induced Production of Matrix Metalloproteinase 
      Both in the Knee Joint of Rat and in Primary Cultured Articular Chondrocytes.
PG  - 163-70
LID - 10.4062/biomolther.2015.217 [doi]
AB  - We examined whether apigenin affects the gene expression, secretion and activity 
      of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular 
      chondrocytes, as well as in vivo production of MMP-3 in the knee joint of rat to 
      evaluate the potential chondroprotective effects of apigenin. Rabbit articular 
      chondrocytes were cultured in a monolayer, and reverse transcription - polymerase 
      chain reaction (RT-PCR) was used to measure interleukin-1beta (IL-1beta)-induced 
      expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with 
      thrombospondin motifs-4 (ADAMTS-4), and ADAMTS-5. In rabbit articular 
      chondrocytes, the effects of apigenin on IL-1beta-induced secretion and proteolytic 
      activity of MMP-3 were investigated using western blot analysis and casein 
      zymography, respectively. The effect of apigenin on MMP-3 protein production was 
      also examined in vivo. In rabbit articular chondrocytes, apigenin inhibited the 
      gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5. Furthermore, 
      apigenin inhibited the secretion and proteolytic activity of MMP-3 in vitro, and 
      inhibited production of MMP-3 protein in vivo. These results suggest that 
      apigenin can regulate the gene expression, secretion, and activity of MMP-3, by 
      directly acting on articular chondrocytes.
FAU - Park, Jin Sung
AU  - Park JS
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of 
      Korea.
FAU - Kim, Dong Kyu
AU  - Kim DK
AD  - Department of Orthopedic Surgery, School of Medicine, Chungnam National 
      University, Daejeon 35015, Republic of Korea.
FAU - Shin, Hyun-Dae
AU  - Shin HD
AD  - Department of Orthopedic Surgery, School of Medicine, Chungnam National 
      University, Daejeon 35015, Republic of Korea.
FAU - Lee, Hyun Jae
AU  - Lee HJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 35015, Republic of Korea.
FAU - Jo, Ho Seung
AU  - Jo HS
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of 
      Korea.
FAU - Jeong, Jin Hoon
AU  - Jeong JH
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of 
      Korea.
FAU - Choi, Young Lac
AU  - Choi YL
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of 
      Korea.
FAU - Lee, Choong Jae
AU  - Lee CJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 35015, Republic of Korea.
FAU - Hwang, Sun-Chul
AU  - Hwang SC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Republic of 
      Korea.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - Biomol Ther (Seoul)
JT  - Biomolecules & therapeutics
JID - 101472832
PMC - PMC4774497
OTO - NOTNLM
OT  - Apigenin
OT  - Chondrocyte
OT  - Metalloproteinase
OT  - Osteoarthritis
EDAT- 2016/02/24 06:00
MHDA- 2016/02/24 06:01
PMCR- 2016/03/01
CRDT- 2016/02/24 06:00
PHST- 2015/12/30 00:00 [received]
PHST- 2016/01/17 00:00 [revised]
PHST- 2016/01/22 00:00 [accepted]
PHST- 2016/02/24 06:00 [entrez]
PHST- 2016/02/24 06:00 [pubmed]
PHST- 2016/02/24 06:01 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - biomolther.2015.217 [pii]
AID - bt-24-163 [pii]
AID - 10.4062/biomolther.2015.217 [doi]
PST - ppublish
SO  - Biomol Ther (Seoul). 2016 Mar 1;24(2):163-70. doi: 10.4062/biomolther.2015.217.