PMID- 26902496
OWN - NLM
STAT- MEDLINE
DCOM- 20170124
LR  - 20180513
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1637
DP  - 2016 Apr 15
TI  - CNQX facilitates inhibitory synaptic transmission in rat hypoglossal nucleus.
PG  - 71-80
LID - S0006-8993(16)30079-8 [pii]
LID - 10.1016/j.brainres.2016.02.020 [doi]
AB  - 6-cyano-7-nitroquinoxaline-2, 3-dione (CNQX) is a most commonly used antagonist 
      of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor in the 
      central nervous system. During the past two decades, studies had demonstrated 
      that CNQX could partially activate AMPA receptors that are located on the 
      hippocampal and cerebellar interneurons, thus subsequently leading to the 
      facilitation of inhibitory transmission. However, whether CNQX could enhance 
      inhibitory synaptic transmission in the hypoglossal nucleus remains elusive. 
      Here, using whole-cell patch-clamp recording in the brainstem slice, we showed 
      that CNQX greatly increased both frequency and amplitude of spontaneous 
      inhibitory postsynaptic currents in the hypoglossal motoneurons, whereas 
      D-(-)-2-Amino-5-phosphonopentanoic acid (D-AP5), N-methyl-D-aspartate (NMDA) 
      receptor antagonist, had no effect on inhibitory synaptic transmission. 
      Application of bicuculline and strychnine further identified that CNQX not only 
      increased GABAergic sIPSCs but also glycinergic one in these motoneurons. Similar 
      enhancement of inhibitory transmission was observed with application of 6, 
      7-dinitroquinoxaline-2, 3-dione (DNQX), a quinoxaline derivative of CNQX, but not 
      with application of GYKI 53655, a non-competitive antagonist of AMPA receptor. In 
      the presence of tetradotoxin, the effect of CNQX on sIPSCs was abolished, 
      suggesting that an increase in presynaptic interneuron spike firing rate induced 
      by CNQX was responsible for the facilitation of sIPSCs. Taken together, these 
      results demonstrated that the excitatory effect of CNQX on presynaptic 
      interneurons triggered enhancement of both GABAergic and glycinergic synaptic 
      transmission within the rat hypoglossal nucleus.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Han, Lei
AU  - Han L
AD  - School of Medicine, Shenzhen University, 518060 Shenzhen, PR China.
FAU - Mu, Shuhua
AU  - Mu S
AD  - School of Medicine, Shenzhen University, 518060 Shenzhen, PR China.
FAU - He, Zhendan
AU  - He Z
AD  - School of Medicine, Shenzhen University, 518060 Shenzhen, PR China.
FAU - Wang, Zhiwei
AU  - Wang Z
AD  - Department of Neurosurgery, Shenzhen Shekou People׳s Hospital, 518060 Shenzhen, 
      PR China.
FAU - Qu, Junle
AU  - Qu J
AD  - College of Optoelectronics Engineering, Shenzhen University, 518060 Shenzhen, PR 
      China.
FAU - Ye, Wencai
AU  - Ye W
AD  - Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New 
      Drugs Research, Jinan University, 510632 Guangzhou, PR China. Electronic address: 
      chywc@yahoo.com.cn.
FAU - Zhang, Jian
AU  - Zhang J
AD  - School of Medicine, Shenzhen University, 518060 Shenzhen, PR China. Electronic 
      address: szuneuroscience@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160218
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (GABA Modulators)
RN  - 0 (Receptors, AMPA)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 6OTE87SCCW (6-Cyano-7-nitroquinoxaline-2,3-dione)
RN  - 76726-92-6 (2-Amino-5-phosphonovalerate)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - 2-Amino-5-phosphonovalerate/pharmacology
MH  - 6-Cyano-7-nitroquinoxaline-2,3-dione/*pharmacology
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Bicuculline/pharmacology
MH  - Cerebellum/drug effects/metabolism
MH  - GABA Modulators
MH  - Hippocampus/drug effects/metabolism
MH  - Hypoglossal Nerve/*drug effects/physiology
MH  - Interneurons/drug effects/physiology
MH  - Membrane Potentials/drug effects
MH  - Motor Neurons/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, AMPA/antagonists & inhibitors/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
MH  - Synaptic Transmission/*drug effects/physiology
OTO - NOTNLM
OT  - AMPA
OT  - CNQX
OT  - Hypoglossal nucleus
OT  - Inhibitory transmission
OT  - Patch-clamp
EDAT- 2016/02/24 06:00
MHDA- 2017/01/25 06:00
CRDT- 2016/02/24 06:00
PHST- 2015/10/22 00:00 [received]
PHST- 2016/01/26 00:00 [revised]
PHST- 2016/02/11 00:00 [accepted]
PHST- 2016/02/24 06:00 [entrez]
PHST- 2016/02/24 06:00 [pubmed]
PHST- 2017/01/25 06:00 [medline]
AID - S0006-8993(16)30079-8 [pii]
AID - 10.1016/j.brainres.2016.02.020 [doi]
PST - ppublish
SO  - Brain Res. 2016 Apr 15;1637:71-80. doi: 10.1016/j.brainres.2016.02.020. Epub 2016 
      Feb 18.