PMID- 26906155
OWN - NLM
STAT- MEDLINE
DCOM- 20160614
LR  - 20181113
IS  - 1552-5783 (Electronic)
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 57
IP  - 2
DP  - 2016 Feb
TI  - Neuroanatomy and Neurochemistry of Mouse Cornea.
PG  - 664-74
LID - 10.1167/iovs.15-18019 [doi]
AB  - PURPOSE: To investigate the entire nerve architecture and content of the two main 
      sensory neuropeptides in mouse cornea to determine if it is a good model with 
      similarities to human corneal innervation. METHODS: Mice aged 1 to 24 weeks were 
      used. The corneas were stained with neuronal-class betaIII-tubulin, calcitonin 
      gene-related peptide (CGRP), and substance P (SP) antibodies; whole-mount images 
      were acquired to build an entire view of corneal innervation. To test the origin 
      of CGRP and SP, trigeminal ganglia (TG) were processed for immunofluorescence. 
      Relative corneal nerve fiber densities or neuron numbers were assessed by 
      computer-assisted analysis. RESULTS: Between 1 and 3 weeks after birth, mouse 
      cornea was mainly composed of a stromal nerve network. At 4 weeks, a whorl-like 
      structure (or vortex) appeared that gradually became more defined. By 8 weeks, 
      anatomy of corneal nerves had reached maturity. Epithelial bundles converged into 
      the central area to form the vortex. The number and pattern of whorl-like 
      structures were different. Subbasal nerve density and nerve terminals were 
      greater in the center than the periphery. Nerve fibers and terminals that were 
      CGRP-positive were more abundant than SP-positive nerves and terminals. In 
      trigeminal ganglia, the number of CGRP-positive neurons significantly outnumbered 
      those positive for SP. CONCLUSIONS: This is the first study to show a complete 
      map of the entire corneal nerves and CGRP and SP sensory neuropeptide 
      distribution in the mouse cornea. This finding shows mouse corneal innervation 
      has many similarities to human cornea and makes the mouse an appropriate model to 
      study pathologies involving corneal nerves.
FAU - He, Jiucheng
AU  - He J
AD  - Louisiana State University Health School of Medicine, New Orleans, Louisiana, 
      United States 2Neuroscience Center of Excellence, Louisiana State University 
      Health, New Orleans, Louisiana, United States 3Department of Ophthalmology, 
      Louisiana State Universi.
FAU - Bazan, Haydee E P
AU  - Bazan HE
AD  - Louisiana State University Health School of Medicine, New Orleans, Louisiana, 
      United States 2Neuroscience Center of Excellence, Louisiana State University 
      Health, New Orleans, Louisiana, United States 3Department of Ophthalmology, 
      Louisiana State Universi.
LA  - eng
GR  - P30 GM103340/GM/NIGMS NIH HHS/United States
GR  - R01 EY019465/EY/NEI NIH HHS/United States
GR  - P30GM103340/GM/NIGMS NIH HHS/United States
GR  - R01 EY19465/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Neuropeptides)
SB  - IM
MH  - Animals
MH  - Cornea/*chemistry/*innervation
MH  - Female
MH  - Male
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Nerve Fibers/*metabolism
MH  - Neuropeptides/*metabolism
MH  - Trigeminal Ganglion/*anatomy & histology/physiology
PMC - PMC4771196
EDAT- 2016/02/26 06:00
MHDA- 2016/06/15 06:00
PMCR- 2016/08/01
CRDT- 2016/02/25 06:00
PHST- 2016/02/25 06:00 [entrez]
PHST- 2016/02/26 06:00 [pubmed]
PHST- 2016/06/15 06:00 [medline]
PHST- 2016/08/01 00:00 [pmc-release]
AID - 2498315 [pii]
AID - IOVS-15-18019 [pii]
AID - 10.1167/iovs.15-18019 [doi]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2016 Feb;57(2):664-74. doi: 10.1167/iovs.15-18019.