PMID- 26907258 OWN - NLM STAT- MEDLINE DCOM- 20161213 LR - 20181113 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 17 IP - 2 DP - 2016 Feb 19 TI - Assessment of Radiation Induced Therapeutic Effect and Cytotoxicity in Cancer Patients Based on Transcriptomic Profiling. PG - 250 LID - 10.3390/ijms17020250 [doi] LID - 250 AB - Toxicity induced by radiation therapy is a curse for cancer patients undergoing treatment. It is imperative to understand and define an ideal condition where the positive effects notably outweigh the negative. We used a microarray meta-analysis approach to measure global gene-expression before and after radiation exposure. Bioinformatic tools were used for pathways, network, gene ontology and toxicity related studies. We found 429 differentially expressed genes at fold change >2 and p-value <0.05. The most significantly upregulated genes were synuclein alpha (SNCA), carbonic anhydrase I (CA1), X-linked Kx blood group (XK), glycophorin A and B (GYPA and GYPB), and hemogen (HEMGN), while downregulated ones were membrane-spanning 4-domains, subfamily A member 1 (MS4A1), immunoglobulin heavy constant mu (IGHM), chemokine (C-C motif) receptor 7 (CCR7), BTB and CNC homology 1 transcription factor 2 (BACH2), and B-cell CLL/lymphoma 11B (BCL11B). Pathway analysis revealed calcium-induced T lymphocyte apoptosis and the role of nuclear factor of activated T-cells (NFAT) in regulation of the immune response as the most inhibited pathways, while apoptosis signaling was significantly activated. Most of the normal biofunctions were significantly decreased while cell death and survival process were activated. Gene ontology enrichment analysis revealed the immune system process as the most overrepresented group under the biological process category. Toxicity function analysis identified liver, kidney and heart to be the most affected organs during and after radiation therapy. The identified biomarkers and alterations in molecular pathways induced by radiation therapy should be further investigated to reduce the cytotoxicity and development of fatigue. FAU - Karim, Sajjad AU - Karim S AD - Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia. skarim1@kau.edu.sa. FAU - Mirza, Zeenat AU - Mirza Z AD - King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia. zmirza1@kau.edu.sa. FAU - Chaudhary, Adeel G AU - Chaudhary AG AD - Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia. adeel.gc@gmail.com. FAU - Abuzenadah, Adel M AU - Abuzenadah AM AD - Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia. adel_abuzenadah@hotmail.com. AD - King Abdullah City for Science and Technology Innovation Center for Personalized Medicine, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia. adel_abuzenadah@hotmail.com. FAU - Gari, Mamdooh AU - Gari M AD - Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia. mgari@kau.edu.sa. FAU - Al-Qahtani, Mohammed H AU - Al-Qahtani MH AD - Center of Excellence in Genomic Medicine Research, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia. mhalqahtani@kau.edu.sa. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160219 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Animals MH - Apoptosis MH - Cell Survival/radiation effects MH - Computational Biology/methods MH - Gene Expression Profiling/*methods MH - Gene Expression Regulation, Neoplastic/radiation effects MH - Gene Ontology MH - Gene Regulatory Networks/*radiation effects MH - Heart/radiation effects MH - Humans MH - Kidney/radiation effects MH - Liver/radiation effects MH - Neoplasms/*genetics/*radiotherapy MH - Oligonucleotide Array Sequence Analysis/*methods PMC - PMC4783980 OTO - NOTNLM OT - cancer OT - microarray OT - pathway analysis OT - radiation therapy OT - toxicity EDAT- 2016/02/26 06:00 MHDA- 2016/12/15 06:00 PMCR- 2016/02/01 CRDT- 2016/02/25 06:00 PHST- 2016/01/17 00:00 [received] PHST- 2016/01/31 00:00 [revised] PHST- 2016/02/03 00:00 [accepted] PHST- 2016/02/25 06:00 [entrez] PHST- 2016/02/26 06:00 [pubmed] PHST- 2016/12/15 06:00 [medline] PHST- 2016/02/01 00:00 [pmc-release] AID - ijms17020250 [pii] AID - ijms-17-00250 [pii] AID - 10.3390/ijms17020250 [doi] PST - epublish SO - Int J Mol Sci. 2016 Feb 19;17(2):250. doi: 10.3390/ijms17020250.