PMID- 26907321
OWN - NLM
STAT- MEDLINE
DCOM- 20161017
LR  - 20181202
IS  - 1660-4601 (Electronic)
IS  - 1661-7827 (Print)
IS  - 1660-4601 (Linking)
VI  - 13
IP  - 3
DP  - 2016 Feb 23
TI  - Effects of in Utero Exposure to Dicyclohexyl Phthalate on Rat Fetal Leydig Cells.
LID - 10.3390/ijerph13030246 [doi]
LID - 246
AB  - Dicyclohexyl phthalate (DCHP) is one of the phthalate plasticizers. The objective 
      of the present study was to investigate the effects of DCHP on fetal Leydig cell 
      distribution and function as well as testis development. Female pregnant Sprague 
      Dawley dams orally received vehicle (corn oil, control) or DCHP (10, 100, and 500 
      mg/kg/day) from gestational day (GD) 12 to GD 21. At GD 21.5, testicular 
      testosterone production, fetal Leydig cell number and distribution, testicular 
      gene and protein expression levels were examined. DCHP administration produced a 
      dose-dependent increase of the incidence of multinucleated gonocytes at >/= 100 
      mg/kg. DCHP dose-dependently increased abnormal fetal Leydig cell aggregation and 
      decreased fetal Leydig cell size, cytoplasmic size, and nuclear size at >/= 10 
      mg/kg. DCHP reduced the expression levels of steroidogenesis-related genes 
      (including Star, Hsd3b1, and Hsd17b3) and testis-descent related gene Insl3 as 
      well as protein levels of 3beta-hydroxysteroid dehydrogenase 1 (HSD3B1) and 
      insulin-like 3 (INSL3) at >/= 10 mg/kg. DCHP significantly inhibited testicular 
      testosterone levels at >/= 100 mg/kg. The results indicate that in utero exposure 
      to DCHP affects the expression levels of fetal Leydig cell steroidogenic genes 
      and results in the occurrence of multinucleated gonocytes and Leydig cell 
      aggregation.
FAU - Li, Xiaoheng
AU  - Li X
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. yaoyao_0111@126.com.
FAU - Chen, Xiaomin
AU  - Chen X
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. xminch@163.com.
FAU - Hu, Guoxin
AU  - Hu G
AD  - Department of Pharmacology, School of Pharmaceutical Sciences, Wenzhou Medical 
      University, Wenzhou 325000, China. hgx@wzmc.edu.cn.
FAU - Li, Linxi
AU  - Li L
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. lilinxi1234@163.com.
FAU - Su, Huina
AU  - Su H
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. suhuina1988@163.com.
FAU - Wang, Yiyan
AU  - Wang Y
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. wyy19880119@163.com.
FAU - Chen, Dongxin
AU  - Chen D
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. gerenshan2@163.com.
FAU - Zhu, Qiqi
AU  - Zhu Q
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. flower3377@163.com.
FAU - Li, Chao
AU  - Li C
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. dishboy@163.com.
FAU - Li, Junwei
AU  - Li J
AD  - Department of Pharmacology, School of Pharmaceutical Sciences, Wenzhou Medical 
      University, Wenzhou 325000, China. gravity172@gmail.com.
FAU - Wang, Mingcang
AU  - Wang M
AD  - Taizhou Hospital of Zhejiang Province, Taizhou 317000, China. wangmc@enzemed.com.
FAU - Lian, Qingquan
AU  - Lian Q
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. lianqingquanmz@163.com.
FAU - Ge, Ren-Shan
AU  - Ge RS
AD  - The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou 
      Medical University, Wenzhou 325027, China. r_ge@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160223
PL  - Switzerland
TA  - Int J Environ Res Public Health
JT  - International journal of environmental research and public health
JID - 101238455
RN  - 0 (Phthalic Acids)
RN  - 0 (Plasticizers)
RN  - 3XMK78S47O (Testosterone)
RN  - 6O7F7IX66E (phthalic acid)
SB  - IM
CIN - Int J Environ Res Public Health. 2016 May 25;13(6):. PMID: 27231928
CIN - Int J Environ Res Public Health. 2016 May 25;13(6):. PMID: 27231929
MH  - Animals
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Fetus/*drug effects
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - Leydig Cells/*drug effects
MH  - Male
MH  - Phthalic Acids/*adverse effects
MH  - Plasticizers/adverse effects
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testis/*drug effects/*growth & development
MH  - Testosterone/*metabolism
PMC - PMC4808909
OTO - NOTNLM
OT  - Leydig cell aggregation
OT  - dicyclohexyl phthalate
OT  - fetal Leydig cell
OT  - testosterone
EDAT- 2016/02/26 06:00
MHDA- 2016/10/19 06:00
PMCR- 2016/03/01
CRDT- 2016/02/25 06:00
PHST- 2016/01/12 00:00 [received]
PHST- 2016/02/15 00:00 [revised]
PHST- 2016/02/16 00:00 [accepted]
PHST- 2016/02/25 06:00 [entrez]
PHST- 2016/02/26 06:00 [pubmed]
PHST- 2016/10/19 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - ijerph13030246 [pii]
AID - ijerph-13-00246 [pii]
AID - 10.3390/ijerph13030246 [doi]
PST - epublish
SO  - Int J Environ Res Public Health. 2016 Feb 23;13(3):246. doi: 
      10.3390/ijerph13030246.