PMID- 26907800
OWN - NLM
STAT- MEDLINE
DCOM- 20170403
LR  - 20170403
IS  - 1525-6049 (Electronic)
IS  - 0886-022X (Linking)
VI  - 38
IP  - 5
DP  - 2016 Jun
TI  - Ozone therapy ameliorates tubulointerstitial inflammation by regulating TLR4 in 
      adenine-induced CKD rats.
PG  - 822-30
LID - 10.3109/0886022X.2016.1143757 [doi]
AB  - Tubulointerstitium inflammation is a common pathway aggravating chronic kidney 
      disease (CKD) progression and the mechanism is partly associated with excessive 
      activation of toll-like receptor 4 (TLR4) in tubulointerstitium. Ozone therapy is 
      demonstrated to alleviate inflammation in some experiments. The aim of this study 
      is to examine whether ozone therapy could ameliorate chronic tubulointerstitium 
      inflammation by suppressing TLR4 in adenine-induced CKD rats. Sprague-Dawley rats 
      were fed with 0.75% adenine-containing diet to induce CKD and tubulointerstitium 
      inflammation injury. Ozone therapy (1.1 mg/kg) was simultaneously administrated 
      by rectal insufflations (i.r.). After 4 weeks, serum and kidney samples were 
      collected for detection. Renal function and systemic electrolyte were detected. 
      Renal pathological changes were assessed by hematoxylin-eosin (H&E) staining and 
      Masson trichrome (MT) staining. Immunohistochemistry, Western blot and Real-time 
      PCR were applied to evaluate tubulointerstitium inflammation as well as the 
      expression of TLR4 and phosphorylated nuclear factor kappa B P65 (p-NF-kappaB P65) in 
      rats. The results showed ozone therapy improved serious renal insufficiency, 
      systemic electrolyte disorder and tubulointerstitium morphology damages in 
      adenine-induced CKD rats. In addition, ozone therapy suppressed excessive 
      activation of TLR4 and p-NF-kappaB P65 in the tubulointerstitium of adenine-induced 
      CKD rats, accompanied by the reduction of inflammation-related cytokines 
      including monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha 
      (TNF-alpha), interleukin-1beta (IL-1beta) and interleukin-6 (IL-6). The protein expression 
      of TLR4 was positively correlated with the protein expression levels of MCP-1 
      (r = 0.7863, p < 0.01) and TNF-alpha (r = 0.7547, p < 0.01) in CKD rats. These 
      findings indicated ozone therapy could attenuate tubulointerstitium inflammation 
      injury in adenine-induced CKD rats and the mechanism might associate with the 
      mediation of TLR4.
FAU - Chen, Zhiyuan
AU  - Chen Z
AD  - a Department of Urology , Renmin Hospital of Wuhan University , Wuhan, Hubei 
      Province , P.R. China.
FAU - Liu, Xiuheng
AU  - Liu X
AD  - a Department of Urology , Renmin Hospital of Wuhan University , Wuhan, Hubei 
      Province , P.R. China.
FAU - Yu, Gang
AU  - Yu G
AD  - a Department of Urology , Renmin Hospital of Wuhan University , Wuhan, Hubei 
      Province , P.R. China.
FAU - Chen, Hui
AU  - Chen H
AD  - a Department of Urology , Renmin Hospital of Wuhan University , Wuhan, Hubei 
      Province , P.R. China.
FAU - Wang, Lei
AU  - Wang L
AD  - a Department of Urology , Renmin Hospital of Wuhan University , Wuhan, Hubei 
      Province , P.R. China.
FAU - Wang, Zhishun
AU  - Wang Z
AD  - a Department of Urology , Renmin Hospital of Wuhan University , Wuhan, Hubei 
      Province , P.R. China.
FAU - Qiu, Tao
AU  - Qiu T
AD  - a Department of Urology , Renmin Hospital of Wuhan University , Wuhan, Hubei 
      Province , P.R. China.
FAU - Weng, Xiaodong
AU  - Weng X
AD  - a Department of Urology , Renmin Hospital of Wuhan University , Wuhan, Hubei 
      Province , P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160224
PL  - England
TA  - Ren Fail
JT  - Renal failure
JID - 8701128
RN  - 0 (NF-kappa B)
RN  - 0 (Oxidants, Photochemical)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - 66H7ZZK23N (Ozone)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Immunohistochemistry
MH  - Inflammation/metabolism
MH  - Kidney/metabolism/pathology
MH  - Kidney Function Tests/methods
MH  - NF-kappa B/metabolism
MH  - Oxidants, Photochemical/pharmacology
MH  - Ozone/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Renal Insufficiency, Chronic/diagnosis/metabolism/therapy
MH  - Toll-Like Receptor 4/*metabolism
MH  - Treatment Outcome
OTO - NOTNLM
OT  - Adenine
OT  - ozone therapy
OT  - rat
OT  - toll-like receptor 4
OT  - tubule-interstitial inflammation
EDAT- 2016/02/26 06:00
MHDA- 2017/04/04 06:00
CRDT- 2016/02/25 06:00
PHST- 2016/02/25 06:00 [entrez]
PHST- 2016/02/26 06:00 [pubmed]
PHST- 2017/04/04 06:00 [medline]
AID - 10.3109/0886022X.2016.1143757 [doi]
PST - ppublish
SO  - Ren Fail. 2016 Jun;38(5):822-30. doi: 10.3109/0886022X.2016.1143757. Epub 2016 
      Feb 24.