PMID- 26908883
OWN - NLM
STAT- MEDLINE
DCOM- 20160607
LR  - 20200220
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 95
IP  - 4
DP  - 2016 Apr
TI  - Transcriptional regulation of cathelicidin genes in chicken bone marrow cells.
PG  - 912-9
LID - 10.3382/ps/pev361 [doi]
AB  - Cathelicidins form a family of vertebrate-specific immune molecules with an 
      evolutionarily conserved gene structure. We analyzed the expression patterns of 
      cathelicidin genes (CAMP, CATH3, and CATHB1) in chicken bone marrow cells (BMCs) 
      and chicken embryonic fibroblasts (CEFs). We found that CAMP and CATHB1 were 
      significantly up-regulated in BMCs, whereas the expression of CATH3 did not 
      differ significantly between BMCs and CEFs. To study the mechanism underlying the 
      up-regulation of cathelicidin genes in BMCs, we predicted the transcription 
      factors (TFs) that bind to the 5'-flanking regions of cathelicidin genes. CEBPA, 
      EBF1, HES1, MSX1, and ZIC3 were up-regulated in BMCs compared to CEFs. 
      Subsequently, when a siRNA-mediated knockdown assay was performed for MSX1, the 
      expression of CAMP and CATHB1 was decreased in BMCs. We also showed that the 
      transcriptional activity of the CAMP promoter was decreased by mutation of the 
      MSX1-binding sites present within the 5'-flanking region of CAMP. These results 
      increase our understanding of the regulatory mechanisms controlling cathelicidin 
      genes in BMCs.
CI  - (c) 2016 Poultry Science Association Inc.
FAU - Lee, Sang In
AU  - Lee SI
AD  - Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, 
      Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea 
      Department of Animal Resource and Science, Dankook University, Cheonan, 
      Choongnam, 330-714, Republic of Korea.
FAU - Jang, Hyun June
AU  - Jang HJ
AD  - College of Pharmacy, Dankook University, 119 Dandae-ro, Cheonan, Chungnam 
      330-714, Republic of Korea.
FAU - Jeon, Mi-hyang
AU  - Jeon MH
AD  - Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, 
      Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of 
      Korea.
FAU - Lee, Mi Ock
AU  - Lee MO
AD  - Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 
      77843-4467.
FAU - Kim, Jeom Sun
AU  - Kim JS
AD  - Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, 
      Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of 
      Korea.
FAU - Jeon, Ik-Soo
AU  - Jeon IS
AD  - Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, 
      Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of 
      Korea.
FAU - Byun, Sung June
AU  - Byun SJ
AD  - Animal Biotechnology Division, National Institute of Animal Science, RDA, 1500, 
      Kongjwipatjwi-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 565-851, Republic of Korea 
      pcs1778@korea.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160215
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Avian Proteins)
RN  - 0 (CMAP27 protein, chicken)
RN  - 0 (Cathelicidins)
SB  - IM
MH  - Animals
MH  - Antimicrobial Cationic Peptides/genetics/metabolism
MH  - Avian Proteins/*genetics/metabolism
MH  - Bone Marrow Cells/metabolism
MH  - Cathelicidins/*genetics/metabolism
MH  - Chick Embryo
MH  - Chickens/*genetics/metabolism
MH  - Fibroblasts/metabolism
MH  - *Gene Expression Regulation
OTO - NOTNLM
OT  - CAMP
OT  - cathelicidin
OT  - chicken
OT  - promoter
OT  - transcription
EDAT- 2016/02/26 06:00
MHDA- 2016/06/09 06:00
CRDT- 2016/02/25 06:00
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/08/06 00:00 [received]
PHST- 2016/02/25 06:00 [entrez]
PHST- 2016/02/26 06:00 [pubmed]
PHST- 2016/06/09 06:00 [medline]
AID - S0032-5791(19)32020-6 [pii]
AID - 10.3382/ps/pev361 [doi]
PST - ppublish
SO  - Poult Sci. 2016 Apr;95(4):912-9. doi: 10.3382/ps/pev361. Epub 2016 Feb 15.