PMID- 26910045
OWN - NLM
STAT- MEDLINE
DCOM- 20160714
LR  - 20220318
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 2
DP  - 2016
TI  - 1alpha,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL 
      Unresponsiveness.
PG  - e0150134
LID - 10.1371/journal.pone.0150134 [doi]
LID - e0150134
AB  - 1,25-Dihydroxyvitamin D3 (Vitamin D) is a naturally synthesized fat soluble 
      vitamin shown to have immunomodulatory, anti-inflammatory and cancer prevention 
      properties in human and murine models. Here, we studied the effects of Vitamin D 
      on the functional abilities of avian T lymphocytes using chicken Interferon 
      (IFN)-gamma ELISPOT assay, BrdU proliferation assay, Annexin V apoptosis assay and 
      PhosFlow for detecting phosphorylated signalling molecules. The results 
      demonstrate that Vitamin D significantly inhibited the abilities of T lymphocytes 
      to produce IFN-gamma and proliferate in vitro (P</=0.05), but retained their ability to 
      undergo degranulation, which is a maker for cytotoxicity of these cells. 
      Similarly, Vitamin D did not inhibit Extracellular signal-Regulated Kinase (ERK) 
      1/2 phosphorylation, a key mediator in T cell signalling, in the stimulated T 
      lymphocytes population, while reduced ERK1/2 phosphorylation levels in the 
      unstimulated cells. Our data provide evidence that Vitamin D has 
      immuno-modulatory properties on chicken T lymphocytes without inducing 
      unresponsiveness and by limiting immuno-pathology can promote protective immunity 
      against infectious diseases of poultry.
FAU - Boodhoo, Nitish
AU  - Boodhoo N
AD  - The Pirbright Institute, Ash Road, Woking, United Kingdom.
FAU - Sharif, Shayan
AU  - Sharif S
AD  - Department of Pathobiology, Ontario Veterinary College, University of Guelph, 
      Guelph, Ontario, Canada.
FAU - Behboudi, Shahriar
AU  - Behboudi S
AD  - The Pirbright Institute, Ash Road, Woking, United Kingdom.
LA  - eng
GR  - BBS/E/I/00001825/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160224
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Animals
MH  - Calcitriol/*pharmacology
MH  - Cell Proliferation/*drug effects
MH  - Chickens/*immunology
MH  - Female
MH  - Humans
MH  - Interferon-gamma/*immunology
MH  - MAP Kinase Signaling System/*drug effects/immunology
MH  - Male
MH  - Mitogen-Activated Protein Kinase 3/immunology
MH  - Phosphorylation/drug effects/immunology
MH  - T-Lymphocytes/*immunology
PMC - PMC4765962
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/02/26 06:00
MHDA- 2016/07/15 06:00
PMCR- 2016/02/24
CRDT- 2016/02/25 06:00
PHST- 2015/08/04 00:00 [received]
PHST- 2016/02/09 00:00 [accepted]
PHST- 2016/02/25 06:00 [entrez]
PHST- 2016/02/26 06:00 [pubmed]
PHST- 2016/07/15 06:00 [medline]
PHST- 2016/02/24 00:00 [pmc-release]
AID - PONE-D-15-34221 [pii]
AID - 10.1371/journal.pone.0150134 [doi]
PST - epublish
SO  - PLoS One. 2016 Feb 24;11(2):e0150134. doi: 10.1371/journal.pone.0150134. 
      eCollection 2016.