PMID- 26912366
OWN - NLM
STAT- MEDLINE
DCOM- 20160325
LR  - 20181113
IS  - 1095-9203 (Electronic)
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 351
IP  - 6277
DP  - 2016 Mar 4
TI  - Isolation of potent neutralizing antibodies from a survivor of the 2014 Ebola 
      virus outbreak.
PG  - 1078-83
LID - 10.1126/science.aad5788 [doi]
AB  - Antibodies targeting the Ebola virus surface glycoprotein (EBOV GP) are 
      implicated in protection against lethal disease, but the characteristics of the 
      human antibody response to EBOV GP remain poorly understood. We isolated and 
      characterized 349 GP-specific monoclonal antibodies (mAbs) from the peripheral B 
      cells of a convalescent donor who survived the 2014 EBOV Zaire outbreak. 
      Remarkably, 77% of the mAbs neutralize live EBOV, and several mAbs exhibit 
      unprecedented potency. Structures of selected mAbs in complex with GP reveal a 
      site of vulnerability located in the GP stalk region proximal to the viral 
      membrane. Neutralizing antibodies targeting this site show potent therapeutic 
      efficacy against lethal EBOV challenge in mice. The results provide a framework 
      for the design of new EBOV vaccine candidates and immunotherapies.
CI  - Copyright (c) 2016, American Association for the Advancement of Science.
FAU - Bornholdt, Zachary A
AU  - Bornholdt ZA
AD  - Department of Immunology and Microbial Science, The Scripps Research Institute, 
      La Jolla, CA 92037, USA.
FAU - Turner, Hannah L
AU  - Turner HL
AD  - Department of Integrative Structural and Computational Biology, The Scripps 
      Research Institute, La Jolla, CA 92037, USA.
FAU - Murin, Charles D
AU  - Murin CD
AD  - Department of Immunology and Microbial Science, The Scripps Research Institute, 
      La Jolla, CA 92037, USA. Department of Integrative Structural and Computational 
      Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
FAU - Li, Wen
AU  - Li W
AD  - Adimab, Lebanon, NH 03766, USA.
FAU - Sok, Devin
AU  - Sok D
AD  - Department of Immunology and Microbial Science, The Scripps Research Institute, 
      La Jolla, CA 92037, USA.
FAU - Souders, Colby A
AU  - Souders CA
AD  - MassBiologics, University of Massachusetts Medical School, Boston, MA 02126, USA.
FAU - Piper, Ashley E
AU  - Piper AE
AD  - U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, 
      USA.
FAU - Goff, Arthur
AU  - Goff A
AD  - U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, 
      USA.
FAU - Shamblin, Joshua D
AU  - Shamblin JD
AD  - U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, 
      USA.
FAU - Wollen, Suzanne E
AU  - Wollen SE
AD  - U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, 
      USA.
FAU - Sprague, Thomas R
AU  - Sprague TR
AD  - U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, 
      USA.
FAU - Fusco, Marnie L
AU  - Fusco ML
AD  - Department of Immunology and Microbial Science, The Scripps Research Institute, 
      La Jolla, CA 92037, USA.
FAU - Pommert, Kathleen B J
AU  - Pommert KB
AD  - Department of Immunology and Microbial Science, The Scripps Research Institute, 
      La Jolla, CA 92037, USA.
FAU - Cavacini, Lisa A
AU  - Cavacini LA
AD  - MassBiologics, University of Massachusetts Medical School, Boston, MA 02126, USA.
FAU - Smith, Heidi L
AU  - Smith HL
AD  - MassBiologics, University of Massachusetts Medical School, Boston, MA 02126, USA.
FAU - Klempner, Mark
AU  - Klempner M
AD  - MassBiologics, University of Massachusetts Medical School, Boston, MA 02126, USA.
FAU - Reimann, Keith A
AU  - Reimann KA
AD  - MassBiologics, University of Massachusetts Medical School, Boston, MA 02126, USA.
FAU - Krauland, Eric
AU  - Krauland E
AD  - Adimab, Lebanon, NH 03766, USA.
FAU - Gerngross, Tillman U
AU  - Gerngross TU
AD  - Adimab, Lebanon, NH 03766, USA.
FAU - Wittrup, Karl D
AU  - Wittrup KD
AD  - Adimab, Lebanon, NH 03766, USA.
FAU - Saphire, Erica Ollmann
AU  - Saphire EO
AD  - Department of Immunology and Microbial Science, The Scripps Research Institute, 
      La Jolla, CA 92037, USA.
FAU - Burton, Dennis R
AU  - Burton DR
AD  - Department of Immunology and Microbial Science, The Scripps Research Institute, 
      La Jolla, CA 92037, USA. Ragon Institute of Massachusetts General Hospital, 
      Massachusetts Institute of Technology, and Harvard University, Cambridge, MA 
      02142, USA.
FAU - Glass, Pamela J
AU  - Glass PJ
AD  - U.S. Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, 
      USA.
FAU - Ward, Andrew B
AU  - Ward AB
AD  - Department of Integrative Structural and Computational Biology, The Scripps 
      Research Institute, La Jolla, CA 92037, USA.
FAU - Walker, Laura M
AU  - Walker LM
AD  - Adimab, Lebanon, NH 03766, USA.
LA  - eng
GR  - UM1 AI100663/AI/NIAID NIH HHS/United States
GR  - U19AI109762/AI/NIAID NIH HHS/United States
GR  - R01 AI067927/AI/NIAID NIH HHS/United States
GR  - R01AI067927/AI/NIAID NIH HHS/United States
GR  - U19 AI109762/AI/NIAID NIH HHS/United States
GR  - T32 AI007244/AI/NIAID NIH HHS/United States
GR  - UM1AI100663/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160218
PL  - United States
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Ebola Vaccines)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (envelope glycoprotein, Ebola virus)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry/*isolation & purification/therapeutic use
MH  - Antibodies, Neutralizing/chemistry/*isolation & purification/therapeutic use
MH  - Antibodies, Viral/chemistry/*isolation & purification/therapeutic use
MH  - Antibody Formation
MH  - Antigen-Antibody Complex/chemistry
MH  - Democratic Republic of the Congo/epidemiology
MH  - Disease Outbreaks
MH  - Ebola Vaccines/immunology/therapeutic use
MH  - Ebolavirus/*immunology
MH  - Hemorrhagic Fever, Ebola/epidemiology/*immunology/therapy
MH  - Humans
MH  - Immunization, Passive
MH  - Mice
MH  - Survivors
MH  - Tissue Donors
MH  - Viral Envelope Proteins/chemistry/*immunology
MH  - Virion/immunology
PMC - PMC4900763
MID - NIHMS788347
EDAT- 2016/02/26 06:00
MHDA- 2016/03/26 06:00
PMCR- 2016/09/04
CRDT- 2016/02/26 06:00
PHST- 2015/10/07 00:00 [received]
PHST- 2016/02/08 00:00 [accepted]
PHST- 2016/02/26 06:00 [entrez]
PHST- 2016/02/26 06:00 [pubmed]
PHST- 2016/03/26 06:00 [medline]
PHST- 2016/09/04 00:00 [pmc-release]
AID - science.aad5788 [pii]
AID - 10.1126/science.aad5788 [doi]
PST - ppublish
SO  - Science. 2016 Mar 4;351(6277):1078-83. doi: 10.1126/science.aad5788. Epub 2016 
      Feb 18.