PMID- 26912816
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Linking)
VI  - 30
IP  - 2
DP  - 2016 Mar-Apr
TI  - Implementation of a Functional Observation Battery for the Assessment of 
      Postoperative Well-being in Rats Subjected to Fimbria-Fornix Transection.
PG  - 77-82
AB  - The postoperative well-being of Wistar rats subjected to fimbria-fornix 
      transections was assessed using a functional observational battery (FOB), 
      including observations of relative body weight change, general condition, fur 
      quality, body posture and movement, appetite, and pica behavior. Fimbria-fornix 
      transected animals (FF), sham-operated animals (Sham), and two non-operated 
      control groups with and without administration of buprenorphine (+BUP and -BUP, 
      respectively) were observed twice daily for seven days after surgery. 
      Buprenorphine (0.4 mg/kg) mixed in a nut paste for voluntary ingestion was 
      supplied twice daily for 84 h to all groups except the -BUP control group 
      starting on the day of surgery. Body weight was slightly decreased 
      postoperatively in both surgical groups (FF and Sham) compared to control groups. 
      The +BUP control group lost weight starting at day four after discontinuation of 
      buprenorphine. Furthermore, the FF group exhibited significantly reduced general 
      condition one day after surgery, with significantly affected body posture and 
      movement for two days after surgery. In addition, mild pica behavior was observed 
      in the FF group during the first postsurgical day. In conclusion, the FOB 
      implemented in the present study appears to be a sensitive and accurate protocol 
      for assessing animal well-being in the experimental setup applied. It is apparent 
      that the FF transection is an invasive procedure that causes mildly adverse 
      postoperative effects on the rats' well-being. We therefore recommend that this 
      FOB is applied as a routine welfare monitoring protocol in experiments using 
      mechanical central nervous system injury models, such as FF transection.
CI  - Copyright (c) 2016 International Institute of Anticancer Research (Dr. John G. 
      Delinassios), All rights reserved.
FAU - Marschner, Linda
AU  - Marschner L
AD  - Department of Psychology, Unit of Cognitive Neuroscience, University of 
      Copenhagen, Copenhagen, Denmark.
FAU - Wogensen, Elise
AU  - Wogensen E
AD  - Department of Psychology, Unit of Cognitive Neuroscience, University of 
      Copenhagen, Copenhagen, Denmark.
FAU - Mogensen, Jesper
AU  - Mogensen J
AD  - Department of Psychology, Unit of Cognitive Neuroscience, University of 
      Copenhagen, Copenhagen, Denmark.
FAU - Abelson, Klas
AU  - Abelson K
AD  - Department of Experimental Medicine, Unit of Cognitive Neuroscience, University 
      of Copenhagen, Copenhagen, Denmark klasab@sund.ku.dk.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
SB  - IM
MH  - Animals
MH  - Behavior, Animal
MH  - Body Weight
MH  - Fornix, Brain/*injuries/surgery
MH  - Male
MH  - Motor Activity
MH  - Postoperative Period
MH  - Rats
MH  - *Recovery of Function
OTO - NOTNLM
OT  - Functional observational battery
OT  - Wister rats
OT  - animal well-being
OT  - fimbria-fornix transection
EDAT- 2016/02/26 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/02/26 06:00
PHST- 2016/02/26 06:00 [entrez]
PHST- 2016/02/26 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 30/2/77 [pii]
PST - ppublish
SO  - In Vivo. 2016 Mar-Apr;30(2):77-82.