PMID- 26914121
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20181202
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 20
IP  - 3
DP  - 2016
TI  - Laryngeal squamous cell carcinoma progression is associated with the NFkappaB 
      signaling pathway regulated by IkappaB kinase beta.
PG  - 467-75
LID - 10281 [pii]
AB  - OBJECTIVE: We aimed to analyze the association of NFkappaB signaling pathway with the 
      carcinogenesis of laryngeal squamous cell carcinoma (LSCC). PATIENTS AND METHODS: 
      Protein array was used to identify the differentially expressed proteins involved 
      in NFkappaB signaling pathway between LSCC cells and normal throat mucosa. 
      Correlation analysis between significantly expressed proteins and clinical 
      characteristics (differentiation, clinical stage, and node metastasis) was 
      performed. The expression of IkappaB kinase-beta (IKK-beta) in Hep-2 cells transfected with 
      IKK-beta-siRNA or pcDNA3.1-IKK-beta was detected both by real-time polymerase chain 
      reaction and western blot. Besides, a tetrazolium-based colorimetric assay (MTT), 
      flow cytometer, and transwell assay were used to examine the proliferation, 
      apoptosis, and migration rate of Hep-2 cells, respectively. RESULTS: Three 
      differentially expressed proteins were identified. Among them, tumor necrosis 
      factor receptor 1 (TNFR1) and IKK-beta were significantly up-regulated (p < 0.01) 
      and Fas-associated via death domain (FADD) was significantly down-regulated (p < 
      0.01). The correlation analysis showed that IKK-beta expression had a significant 
      association with differentiation, clinical stage, and node metastasis (p < 0.05). 
      Besides, high expressed IKK-beta resulted in significantly increased proliferation 
      and migration rate (p < 0.05). Reversely, Hep-2 cells transfected with 
      IKK-beta-siRNA showed significantly lower proliferation and migration rate (p < 
      0.05) and significantly higher apoptosis rate (p < 0.05) than normal cells. 
      CONCLUSIONS: The NFkappaB signaling pathway involved TNFR1, IKK-beta, and FADD is 
      significantly associated with the development of LSCC. Over-expressed IKK-beta 
      efficiently inhibits cell apoptosis and has positive effects on cell 
      proliferation and migration.
FAU - Li, X-T
AU  - Li XT
AD  - Department of Otolaryngology-Head and Neck Surgery, The First Hospital of China 
      Medical University, Shenyang, China. xiaotianlidoc@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - EC 2.7.11.10 (IKBKB protein, human)
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/*biosynthesis/genetics
MH  - Carcinoma, Squamous Cell/diagnosis/genetics/*metabolism
MH  - *Disease Progression
MH  - Female
MH  - Hep G2 Cells
MH  - Humans
MH  - I-kappa B Kinase/*physiology
MH  - Laryngeal Neoplasms/diagnosis/genetics/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - NF-kappa B/*biosynthesis
MH  - RNA, Small Interfering/genetics
MH  - Signal Transduction/physiology
EDAT- 2016/02/26 06:00
MHDA- 2016/10/14 06:00
CRDT- 2016/02/26 06:00
PHST- 2016/02/26 06:00 [entrez]
PHST- 2016/02/26 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
AID - 10281 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2016;20(3):467-75.