PMID- 26917440
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20180612
IS  - 1544-0591 (Electronic)
IS  - 0022-0345 (Linking)
VI  - 95
IP  - 6
DP  - 2016 Jun
TI  - Osteoprotective Effects of Estrogen in the Maxillary Bone Depend on ERalpha.
PG  - 689-96
LID - 10.1177/0022034516633154 [doi]
AB  - Estrogen deficiency results in disruption of maxillary alveolar bone 
      microarchitecture. Most of the actions of estrogen in long bones occur via 
      estrogen receptor alpha (ERalpha). However, the function of ERalpha in the maxillary bone has 
      not been defined. We aimed to investigate the role and underlying mechanisms of 
      ERalpha in the physiological and mechanically induced alveolar bone remodeling in 
      female and male mice. Wild-type (WT) and ERalpha(-/-) (ERKOalpha) mice were subjected to 
      mechanically stimulated bone remodeling by inducing orthodontic tooth movement 
      (OTM). The maxillary bone was analyzed using histomorphometric analysis, 
      micro-computed tomography, quantitative polymerase chain reaction, and 
      energy-dispersive spectroscopy. Bone marrow cells (BMCs) from WT and ERKOalpha mice 
      were tested for their capacity to differentiate into osteoblasts and osteoclasts. 
      Both male and female ERKOalpha mice exhibited marked reduction of alveolar bone mass 
      and increased OTM. This response was associated with an increased number of 
      osteoclasts and reduced number of apoptotic cells and osteoblasts in the 
      periodontium and alveolar bone. Consistently, ERKOalpha mice exhibited lower levels 
      of calcium in bone and increased expression of IL-33 (interleukin-33), TNF-alpha 
      (tumor necrosis factor alpha), and IL-1beta (interleukin-1beta) and decreased expression of 
      dentin matrix acidic phosphoprotein and alkaline phosphatase in periodontal 
      tissues. Moreover, the differentiation of osteoclasts and osteoblasts in vitro 
      was significantly higher in BMCs obtained from ERKOalpha. ERalpha is required to maintain 
      the microarchitecture of maxillary alveolar bone. This process is linked to bone 
      cell differentiation and apoptosis, as well as local production of inflammatory 
      molecules such as IL-33, TNF-alpha, and IL-1beta.
CI  - (c) International & American Associations for Dental Research 2016.
FAU - Macari, S
AU  - Macari S
AD  - Department of Oral Pathology and Surgery, Faculty of Dentistry, Federal 
      University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
FAU - Ajay Sharma, L
AU  - Ajay Sharma L
AD  - Department of Anatomy, University of Otago, Dunedin, New Zealand.
FAU - Wyatt, A
AU  - Wyatt A
AD  - Department of Anatomy, University of Otago, Dunedin, New Zealand.
FAU - Knowles, P
AU  - Knowles P
AD  - Department of Anatomy, University of Otago, Dunedin, New Zealand.
FAU - Szawka, R E
AU  - Szawka RE
AD  - Department of Physiology and Biophysics, Biological Science Institute, Federal 
      University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
FAU - Garlet, G P
AU  - Garlet GP
AD  - Department of Biological Sciences, School of Dentistry of Bauru, Sao Paulo 
      University, Bauru, SP, Brazil.
FAU - Grattan, D R
AU  - Grattan DR
AD  - Department of Anatomy, University of Otago, Dunedin, New Zealand.
FAU - Dias, G J
AU  - Dias GJ
AD  - Department of Anatomy, University of Otago, Dunedin, New Zealand.
FAU - Silva, T A
AU  - Silva TA
AD  - Department of Oral Pathology and Surgery, Faculty of Dentistry, Federal 
      University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil 
      tarcilia@ufmg.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160225
PL  - United States
TA  - J Dent Res
JT  - Journal of dental research
JID - 0354343
RN  - 0 (Dmp1 protein, mouse)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-33)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Alveolar Bone Loss/*prevention & control
MH  - Animals
MH  - Apoptosis
MH  - Bone Marrow Cells/physiology
MH  - Bone Remodeling
MH  - Calcium/metabolism
MH  - Cell Differentiation
MH  - Estrogen Receptor alpha/*physiology
MH  - Extracellular Matrix Proteins/metabolism
MH  - Female
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-33/metabolism
MH  - Male
MH  - Maxilla/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Osteoblasts/metabolism
MH  - Osteoclasts/metabolism
MH  - Ovariectomy
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Spectrometry, X-Ray Emission
MH  - Tooth Movement Techniques
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - alveolar bone loss
OT  - bone remodeling
OT  - estrogen receptor alpha
OT  - interleukin-33
OT  - maxilla
OT  - osteoporosis
EDAT- 2016/02/27 06:00
MHDA- 2017/05/23 06:00
CRDT- 2016/02/27 06:00
PHST- 2016/02/27 06:00 [entrez]
PHST- 2016/02/27 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
AID - 0022034516633154 [pii]
AID - 10.1177/0022034516633154 [doi]
PST - ppublish
SO  - J Dent Res. 2016 Jun;95(6):689-96. doi: 10.1177/0022034516633154. Epub 2016 Feb 
      25.