PMID- 26918636
OWN - NLM
STAT- MEDLINE
DCOM- 20160720
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 2
DP  - 2016
TI  - Spouse-to-Spouse Transmission and Evolution of Hypervariable Region 1 and 5' 
      Untranslated Region of Hepatitis C Virus Analyzed by Next-Generation Sequencing.
PG  - e0150311
LID - 10.1371/journal.pone.0150311 [doi]
LID - e0150311
AB  - Hepatitis C virus (HCV) transmission between spouses remains poorly 
      characterized, largely due to the limited availability of samples from the early 
      stage of infection, as well as methodological constraints. A fifty-eight year-old 
      male developed acute hepatitis C infection and his 53-year old spouse has been 
      HCV-positive for over 10 years. Serum samples were collected from both at the 
      time of acute hepatitis C diagnosis in male (baseline) and then at 9 and 13 
      months. Hypervariable region 1 (HVR1) and 5' untranslated region (5'UTR) 
      sequences were amplified and subjected to next generation sequencing (NGS) using 
      a pyrosequencing platform. Genetic variants were inferred by Shorah 
      reconstruction method and compared by phylogenetic and sequence diversity 
      analysis. As the sequencing error of the procedure was previously determined to 
      be </= 1.5%, the analysis was conducted with and without the 1.5% cut-off with 
      regard to the frequency of variants. No identical HVR1 variants were identified 
      in spouses at baseline and follow-up samples regardless whether the cut-off was 
      applied or not. However, there was high similarity (98.3%) between a minor 
      baseline donor variant (1.7% frequency) and the most abundant baseline recipient 
      variant (62.5% frequency). Furthermore, donor and recipient strains clustered 
      together when compared to 10 control subjects from the same area and infected 
      with the same HCV subtype. There was an increase in HVR1 complexity (number of 
      genetic variants) over time in both spouses. In contrast, the 5'UTR region was 
      stable and of low complexity throughout the study. In conclusion, intrafamilial 
      HCV transmission may be established by a very minor variant and investigation of 
      this phenomenon requires high-sensitivity assays, such as NGS.
FAU - Caraballo Cortes, Kamila
AU  - Caraballo Cortes K
AD  - Department of Immunopathology of Infectious and Parasitic Diseases, Medical 
      University of Warsaw, Warsaw, Poland.
FAU - Zagordi, Osvaldo
AU  - Zagordi O
AD  - Institute of Medical Virology, University of Zurich, Zurich, Switzerland.
FAU - Jablonska, Joanna
AU  - Jablonska J
AD  - Clinics of Infectious, Tropical Diseases and Hepatology, Medical University of 
      Warsaw, Warsaw, Poland.
FAU - Pawelczyk, Agnieszka
AU  - Pawelczyk A
AD  - Department of Immunopathology of Infectious and Parasitic Diseases, Medical 
      University of Warsaw, Warsaw, Poland.
FAU - Kubisa, Natalia
AU  - Kubisa N
AD  - Department of Immunopathology of Infectious and Parasitic Diseases, Medical 
      University of Warsaw, Warsaw, Poland.
FAU - Perlejewski, Karol
AU  - Perlejewski K
AD  - Department of Immunopathology of Infectious and Parasitic Diseases, Medical 
      University of Warsaw, Warsaw, Poland.
FAU - Bukowska-Osko, Iwona
AU  - Bukowska-Osko I
AD  - Department of Immunopathology of Infectious and Parasitic Diseases, Medical 
      University of Warsaw, Warsaw, Poland.
FAU - Ploski, Rafal
AU  - Ploski R
AD  - Department of Medical Genetics, Medical University of Warsaw, Warsaw, Poland.
FAU - Radkowski, Marek
AU  - Radkowski M
AD  - Department of Immunopathology of Infectious and Parasitic Diseases, Medical 
      University of Warsaw, Warsaw, Poland.
FAU - Laskus, Tomasz
AU  - Laskus T
AD  - Department of Immunopathology of Infectious and Parasitic Diseases, Medical 
      University of Warsaw, Warsaw, Poland.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160226
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (5' Untranslated Regions)
RN  - 0 (DNA, Complementary)
RN  - 0 (Epitopes)
RN  - 0 (HVR1 protein, Hepatitis C virus)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - 5' Untranslated Regions/*genetics
MH  - Amino Acid Sequence
MH  - DNA, Complementary/genetics
MH  - Epitopes/genetics/immunology
MH  - Evolution, Molecular
MH  - Female
MH  - Follow-Up Studies
MH  - Hepacivirus/*genetics/immunology
MH  - Hepatitis C/*transmission/virology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Viral/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - *Spouses
MH  - Viral Proteins/*genetics
PMC - PMC4769329
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2016/02/27 06:00
MHDA- 2016/07/21 06:00
PMCR- 2016/02/26
CRDT- 2016/02/27 06:00
PHST- 2015/07/30 00:00 [received]
PHST- 2016/02/11 00:00 [accepted]
PHST- 2016/02/27 06:00 [entrez]
PHST- 2016/02/27 06:00 [pubmed]
PHST- 2016/07/21 06:00 [medline]
PHST- 2016/02/26 00:00 [pmc-release]
AID - PONE-D-15-33285 [pii]
AID - 10.1371/journal.pone.0150311 [doi]
PST - epublish
SO  - PLoS One. 2016 Feb 26;11(2):e0150311. doi: 10.1371/journal.pone.0150311. 
      eCollection 2016.