PMID- 26921439
OWN - NLM
STAT- MEDLINE
DCOM- 20170630
LR  - 20200930
IS  - 1522-1539 (Electronic)
IS  - 0363-6135 (Linking)
VI  - 310
IP  - 9
DP  - 2016 May 1
TI  - Comparison of sympathetic nerve activity normalization procedures in conscious 
      rabbits.
PG  - H1222-32
LID - 10.1152/ajpheart.00866.2015 [doi]
AB  - One of the main constraints associated with recording sympathetic nerve activity 
      (SNA) in both humans and experimental animals is that microvolt values reflect 
      characteristics of the recording conditions and limit comparisons between 
      different experimental groups. The nasopharyngeal response has been validated for 
      normalizing renal SNA (RSNA) in conscious rabbits, and in humans muscle SNA is 
      normalized to the maximum burst in the resting period. We compared these two 
      methods of normalization to determine whether either could detect elevated RSNA 
      in hypertensive rabbits compared with normotensive controls. We also tested 
      whether either method eliminated differences based only on different recording 
      conditions by separating RSNA of control (sham) rabbits into two groups with low 
      or high microvolts. Hypertension was induced by 5 wk of renal clipping (2K1C), 3 
      wk of high-fat diet (HFD), or 3 mo infusion of a low dose of angiotensin (ANG 
      II). Normalization to the nasopharyngeal response revealed RSNA that was 88, 51, 
      and 34% greater in 2K1C, HFD, and ANG II rabbits, respectively, than shams (P < 
      0.05), but normalization to the maximum burst showed no differences. The RSNA 
      baroreflex followed a similar pattern whether RSNA was expressed in microvolts or 
      normalized. Both methods abolished the difference between low and high microvolt 
      RSNA. These results suggest that maximum burst amplitude is a useful technique 
      for minimizing differences between recording conditions but is unable to detect 
      real differences between groups. We conclude that the nasopharyngeal reflex is 
      the superior method for normalizing sympathetic recordings in conscious rabbits.
CI  - Copyright (c) 2016 the American Physiological Society.
FAU - Burke, Sandra L
AU  - Burke SL
AD  - Neuropharmacology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, 
      Victoria, Australia; and.
FAU - Lim, Kyungjoon
AU  - Lim K
AD  - Neuropharmacology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, 
      Victoria, Australia; and.
FAU - Moretti, John-Luis
AU  - Moretti JL
AD  - Neuropharmacology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, 
      Victoria, Australia; and.
FAU - Head, Geoffrey A
AU  - Head GA
AD  - Neuropharmacology Laboratory, Baker IDI Heart and Diabetes Institute, Melbourne, 
      Victoria, Australia; and Department of Pharmacology, Monash University, Clayton, 
      Victoria, Australia geoff.head@baker.edu.au.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160226
PL  - United States
TA  - Am J Physiol Heart Circ Physiol
JT  - American journal of physiology. Heart and circulatory physiology
JID - 100901228
RN  - 11128-99-7 (Angiotensin II)
SB  - IM
MH  - Action Potentials
MH  - Angiotensin II
MH  - Animals
MH  - Arterial Pressure
MH  - *Baroreflex
MH  - Blood Pressure Determination/methods
MH  - Calibration
MH  - Consciousness
MH  - Constriction
MH  - Diet, High-Fat
MH  - Disease Models, Animal
MH  - Electrodiagnosis/*methods/standards
MH  - Heart Rate
MH  - Hypertension/etiology/*physiopathology
MH  - Kidney/*innervation
MH  - Male
MH  - Models, Animal
MH  - Muscle, Skeletal/*innervation
MH  - Nasopharynx/*innervation
MH  - Rabbits
MH  - Renal Artery/physiopathology/surgery
MH  - Reproducibility of Results
MH  - Signal Processing, Computer-Assisted
MH  - Sympathetic Nervous System/*physiopathology
MH  - Telemetry/methods
MH  - Time Factors
OTO - NOTNLM
OT  - blood pressure
OT  - hypertension
OT  - nasopharyngeal stimulation
OT  - normalization method
OT  - rabbit
EDAT- 2016/02/28 06:00
MHDA- 2017/07/01 06:00
CRDT- 2016/02/28 06:00
PHST- 2015/11/06 00:00 [received]
PHST- 2016/02/25 00:00 [accepted]
PHST- 2016/02/28 06:00 [entrez]
PHST- 2016/02/28 06:00 [pubmed]
PHST- 2017/07/01 06:00 [medline]
AID - ajpheart.00866.2015 [pii]
AID - 10.1152/ajpheart.00866.2015 [doi]
PST - ppublish
SO  - Am J Physiol Heart Circ Physiol. 2016 May 1;310(9):H1222-32. doi: 
      10.1152/ajpheart.00866.2015. Epub 2016 Feb 26.