PMID- 26924643
OWN - NLM
STAT- MEDLINE
DCOM- 20171201
LR  - 20181113
IS  - 1440-1711 (Electronic)
IS  - 0818-9641 (Linking)
VI  - 94
IP  - 7
DP  - 2016 Aug
TI  - Artificial antigen-presenting cells expressing HLA class II molecules as an 
      effective tool for amplifying human specific memory CD4(+) T cells.
PG  - 662-72
LID - 10.1038/icb.2016.25 [doi]
AB  - Owing to their multiple immune functions, CD4(+) T cells are of major interest 
      for immunotherapy in chronic viral infections and cancer, as well as for severe 
      autoimmune diseases and transplantation. Therefore, standardized methods allowing 
      rapid generation of a large number of CD4(+) T cells for adoptive immunotherapy 
      are still awaited. We constructed stable artificial antigen-presenting cells 
      (AAPCs) derived from mouse fibroblasts. They were genetically modified to express 
      human leukocyte antigen (HLA)-DR molecules and the human accessory molecules 
      B7.1, Intercellular adhesion molecule-1 (ICAM-1) and lymphocyte 
      function-associated antigen-3 (LFA-3). AAPCs expressing HLA-DR1, HLA-DR15 or 
      HLA-DR51 molecules and loaded with peptides derived from influenza hemagglutinin 
      (HA), myelin basic protein (MBP) or factor VIII, respectively, activated specific 
      CD4(+) T-cell clones more effectively than Epstein-Barr virus (EBV)-transformed B 
      cells. We also showed that AAPCs were able to take up and process whole Ag 
      proteins, and present epitopes to specific T cells. In primary cultures, AAPCs 
      loaded with HA peptide allowed generation of specific Th1 lymphocytes from 
      healthy donors as demonstrated by tetramer and intracellular cytokine staining. 
      Although AAPCs were less effective than autologous peripheral blood mononuclear 
      cells (PBMCs) to stimulate CD4(+) T cells in primary culture, AAPCs were more 
      potent to reactivate and expand memory Th1 cells in a strictly Ag-dependent 
      manner. As the availability of autologous APCs is limited, the AAPC system 
      represents a stable and reliable tool to achieve clinically relevant numbers of 
      CD4(+) T cells for adoptive immunotherapy. For fundamental research in 
      immunology, AAPCs are also useful to decipher mechanisms involved in the 
      development of human CD4 T-cell responses.
FAU - Garnier, Anthony
AU  - Garnier A
AD  - Inserm U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, 
      Caen, France.
AD  - Universite de Caen Basse-Normandie, UFR Medecine, Caen, France.
FAU - Hamieh, Mohamad
AU  - Hamieh M
AD  - Inserm U1079, Institute for Research and Innovation in Biomedicine (IRIB), Rouen 
      University, Rouen, France.
FAU - Drouet, Aurelie
AU  - Drouet A
AD  - Inserm U1079, Institute for Research and Innovation in Biomedicine (IRIB), Rouen 
      University, Rouen, France.
FAU - Leprince, Jerome
AU  - Leprince J
AD  - Inserm U982, Institute for Research and Innovation in Biomedicine (IRIB), Rouen 
      University, Rouen, France.
FAU - Vivien, Denis
AU  - Vivien D
AD  - Inserm U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, 
      Caen, France.
AD  - Universite de Caen Basse-Normandie, UFR Medecine, Caen, France.
FAU - Frebourg, Thierry
AU  - Frebourg T
AD  - Inserm U1079, Institute for Research and Innovation in Biomedicine (IRIB), Rouen 
      University, Rouen, France.
AD  - Department of Genetics, Rouen University Hospital, Rouen, France.
FAU - Le Mauff, Brigitte
AU  - Le Mauff B
AD  - Inserm U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, 
      Caen, France.
AD  - Universite de Caen Basse-Normandie, UFR Medecine, Caen, France.
AD  - CHU Caen, Department of Immunology and Immunopathology, Caen, France.
AD  - Etablissement Francais du Sang, Normandie, Caen, France.
FAU - Latouche, Jean-Baptiste
AU  - Latouche JB
AD  - Inserm U1079, Institute for Research and Innovation in Biomedicine (IRIB), Rouen 
      University, Rouen, France.
AD  - Department of Genetics, Rouen University Hospital, Rouen, France.
FAU - Toutirais, Olivier
AU  - Toutirais O
AD  - Inserm U919, Serine Proteases and Pathophysiology of the Neurovascular Unit, 
      Caen, France.
AD  - Universite de Caen Basse-Normandie, UFR Medecine, Caen, France.
AD  - CHU Caen, Department of Immunology and Immunopathology, Caen, France.
AD  - Etablissement Francais du Sang, Normandie, Caen, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160229
PL  - United States
TA  - Immunol Cell Biol
JT  - Immunology and cell biology
JID - 8706300
RN  - 0 (Epitopes)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Antigen-Presenting Cells/*immunology
MH  - Artificial Cells/*immunology
MH  - CD4-Positive T-Lymphocytes/cytology/*immunology
MH  - Cell Proliferation
MH  - Epitopes/immunology
MH  - Histocompatibility Antigens Class II/*metabolism
MH  - Humans
MH  - *Immunologic Memory
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Peptides/metabolism
MH  - Phenotype
MH  - Tissue Donors
EDAT- 2016/03/01 06:00
MHDA- 2017/12/02 06:00
CRDT- 2016/03/01 06:00
PHST- 2015/10/13 00:00 [received]
PHST- 2016/02/21 00:00 [revised]
PHST- 2016/02/22 00:00 [accepted]
PHST- 2016/03/01 06:00 [entrez]
PHST- 2016/03/01 06:00 [pubmed]
PHST- 2017/12/02 06:00 [medline]
AID - icb201625 [pii]
AID - 10.1038/icb.2016.25 [doi]
PST - ppublish
SO  - Immunol Cell Biol. 2016 Aug;94(7):662-72. doi: 10.1038/icb.2016.25. Epub 2016 Feb 
      29.