PMID- 26924701
OWN - NLM
STAT- MEDLINE
DCOM- 20170227
LR  - 20190601
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 23
IP  - 11
DP  - 2016 Jun
TI  - The effects of CYP1A inhibition on alkyl-phenanthrene metabolism and 
      embryotoxicity in marine medaka (Oryzias melastigma).
PG  - 11289-11297
LID - 10.1007/s11356-016-6098-2 [doi]
AB  - Alkylated polycyclic aromatic hydrocarbons (alkyl-PAHs) are the predominant form 
      of PAHs in crude oils, of which, 3-5 ring alkyl-PAH may cause dioxin-like 
      toxicity to early life stages of fish. Retene (7-isopropyl-1-methylphenanthrene), 
      a typical alkyl-phenanthrene compound, can be more toxic than phenanthrene, and 
      the mechanism of retene toxicity is likely related to its rapid biotransformation 
      by cytochrome P450 (CYP) enzymes to metabolites with a wide array of structures 
      and potential toxicities. Here, we investigated how alpha-naphthoflavone (ANF), a 
      cytochrome P450 1A (CYP1A) inhibitor, affected the embryotoxicity of retene and 
      the role that CYP1A inhibition may play in the interactions. Marine medaka 
      (Oryzias melastigma) embryos were exposed, separately or together, to 200 mug/L 
      retene with 0, 5, 10, 100, and 200 mug/L ANF for 14 days. The results showed that 
      ANF significantly inhibited the induction of CYP1A activity by retene; however, 
      ANF interacted with retene to induce significant developmental toxicity and 
      genotoxicity at 10, 100, and 200 mug/L (p < 0.01). Tissue concentrations of retene 
      and its metabolites and lipid hydroperoxide (LPO) activity also increased, 
      whereas the inhibition of the glutathione S-transferase (GST) activity and the 
      alteration in metabolic profiles of retene were observed. The interactions of 
      retene with ANF indicate that CYP1A inhibition was possibly act through different 
      mechanisms to produce similar developmental effects and genotoxicity. Retene 
      metabolites and altered metabolic profile were likely responsible for retene 
      embryotoxicity to marine medaka. Therefore, elevated toxicity of 
      alkyl-phenanthrene under CYP1A inhibitor suggested that the ecotoxicity of PAHs 
      in coastal water may have underestimated the threat of PAHs to fish or ecosystem.
FAU - Mu, Jingli
AU  - Mu J
AD  - Key Laboratory for Ecological Environment in Coastal Areas (State Oceanic 
      Administration, SOA), National Marine Environmental Monitoring Center, Dalian, 
      116023, China.
FAU - Jin, Fei
AU  - Jin F
AD  - Key Laboratory for Ecological Environment in Coastal Areas (State Oceanic 
      Administration, SOA), National Marine Environmental Monitoring Center, Dalian, 
      116023, China.
FAU - Wang, Juying
AU  - Wang J
AD  - Key Laboratory for Ecological Environment in Coastal Areas (State Oceanic 
      Administration, SOA), National Marine Environmental Monitoring Center, Dalian, 
      116023, China. jywang@nmemc.org.cn.
FAU - Wang, Ying
AU  - Wang Y
AD  - Key Laboratory for Ecological Environment in Coastal Areas (State Oceanic 
      Administration, SOA), National Marine Environmental Monitoring Center, Dalian, 
      116023, China.
FAU - Cong, Yi
AU  - Cong Y
AD  - Key Laboratory for Ecological Environment in Coastal Areas (State Oceanic 
      Administration, SOA), National Marine Environmental Monitoring Center, Dalian, 
      116023, China.
LA  - eng
PT  - Journal Article
DEP - 20160229
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Benzoflavones)
RN  - 0 (Petroleum)
RN  - 0 (Phenanthrenes)
RN  - 0 (Water Pollutants, Chemical)
RN  - 0W2D2E1P9Q (retene)
RN  - 604-59-1 (alpha-naphthoflavone)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Animals
MH  - Benzoflavones/pharmacology
MH  - Cytochrome P-450 CYP1A1/*antagonists & inhibitors
MH  - Dose-Response Relationship, Drug
MH  - Embryo, Nonmammalian/abnormalities/*drug effects/enzymology
MH  - Glutathione Transferase/metabolism
MH  - Oryzias/embryology/*metabolism
MH  - Petroleum/analysis/metabolism
MH  - Phenanthrenes/analysis/pharmacokinetics/*toxicity
MH  - Water Pollutants, Chemical/analysis/pharmacokinetics/*toxicity
OTO - NOTNLM
OT  - Alkylated polycyclic aromatic hydrocarbons
OT  - CYP1A activity
OT  - Marine medaka
OT  - Metabolism
OT  - Synergistic toxicity
EDAT- 2016/03/01 06:00
MHDA- 2017/02/28 06:00
CRDT- 2016/03/01 06:00
PHST- 2015/10/29 00:00 [received]
PHST- 2016/01/11 00:00 [accepted]
PHST- 2016/03/01 06:00 [entrez]
PHST- 2016/03/01 06:00 [pubmed]
PHST- 2017/02/28 06:00 [medline]
AID - 10.1007/s11356-016-6098-2 [pii]
AID - 10.1007/s11356-016-6098-2 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2016 Jun;23(11):11289-11297. doi: 
      10.1007/s11356-016-6098-2. Epub 2016 Feb 29.