PMID- 26927318
OWN - NLM
STAT- MEDLINE
DCOM- 20180118
LR  - 20180213
IS  - 1460-2423 (Electronic)
IS  - 0959-6658 (Linking)
VI  - 26
IP  - 7
DP  - 2016 Jul
TI  - Bacterial synthesis of polysialic acid lactosides in recombinant Escherichia coli 
      K-12.
PG  - 723-731
LID - 10.1093/glycob/cww027 [doi]
AB  - Bacterial polysialyltransferases (PSTs) are processive enzymes involved in the 
      synthesis of polysialic capsular polysaccharides. They can also synthesize 
      polysialic acid in vitro from disialylated and trisialylated lactoside acceptors, 
      which are the carbohydrate moieties of GD3 and GT3 gangliosides, respectively. 
      Here, we engineered a non-pathogenic Escherichia coli strain that overexpresses 
      recombinant sialyltransferases and sialic acid synthesis genes and can convert an 
      exogenous lactoside into polysialyl lactosides. Several PSTs were assayed for 
      their ability to synthesize polysialyl lactosides in the recombinant strains. 
      Fed-batch cultures produced alpha-2,8 polysialic acid or alternate alpha-2,8-2,9 
      polysialic acid in quantities reaching several grams per liter. Bacterial culture 
      in the presence of propargyl-beta-lactoside as the exogenous acceptor led to the 
      production of conjugatable polysaccharides by means of copper-assisted click 
      chemistry.
CI  - (c) The Author 2016. Published by Oxford University Press. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Richard, Emeline
AU  - Richard E
AD  - Univ. Grenoble Alpes, CERMAV, Grenoble F-38000, France.
AD  - CNRS, CERMAV, Grenoble F-38000, France.
FAU - Buon, Laurine
AU  - Buon L
AD  - Univ. Grenoble Alpes, CERMAV, Grenoble F-38000, France.
AD  - CNRS, CERMAV, Grenoble F-38000, France.
FAU - Drouillard, Sophie
AU  - Drouillard S
AD  - Univ. Grenoble Alpes, CERMAV, Grenoble F-38000, France.
AD  - CNRS, CERMAV, Grenoble F-38000, France.
FAU - Fort, Sebastien
AU  - Fort S
AD  - Univ. Grenoble Alpes, CERMAV, Grenoble F-38000, France.
AD  - CNRS, CERMAV, Grenoble F-38000, France.
FAU - Priem, Bernard
AU  - Priem B
AD  - Univ. Grenoble Alpes, CERMAV, Grenoble F-38000, France.
AD  - CNRS, CERMAV, Grenoble F-38000, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160229
PL  - England
TA  - Glycobiology
JT  - Glycobiology
JID - 9104124
RN  - 0 (Gangliosides)
RN  - 0 (Glycosides)
RN  - 0 (Lactosylceramides)
RN  - 0 (Polysaccharides, Bacterial)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sialic Acids)
RN  - 0 (lactosides)
RN  - 0 (polysialic acid)
RN  - 73904-48-0 (trisialosyllactosylceramide)
RN  - EC 2.4.99.- (Sialyltransferases)
SB  - IM
MH  - Escherichia coli K12/genetics
MH  - Gangliosides
MH  - Gene Expression Regulation, Enzymologic/genetics
MH  - Glycosides/*biosynthesis/genetics
MH  - Glycosylation
MH  - Lactosylceramides
MH  - Polysaccharides, Bacterial/biosynthesis/genetics
MH  - Recombinant Proteins/genetics
MH  - Sialic Acids/*biosynthesis/genetics
MH  - Sialyltransferases/*genetics
OTO - NOTNLM
OT  - bacterial glycosylation
OT  - click chemistry
OT  - glyco-engineering
OT  - polysialic acids
EDAT- 2016/03/02 06:00
MHDA- 2018/01/19 06:00
CRDT- 2016/03/02 06:00
PHST- 2015/10/05 00:00 [received]
PHST- 2016/02/24 00:00 [accepted]
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2018/01/19 06:00 [medline]
AID - cww027 [pii]
AID - 10.1093/glycob/cww027 [doi]
PST - ppublish
SO  - Glycobiology. 2016 Jul;26(7):723-731. doi: 10.1093/glycob/cww027. Epub 2016 Feb 
      29.