PMID- 26927370
OWN - NLM
STAT- MEDLINE
DCOM- 20160719
LR  - 20160301
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 32
IP  - 2
DP  - 2016 Feb
TI  - [The recombinant monocyte chemotactic protein 1 expressed in HEK293T cells 
      promotes migration of macrophages].
PG  - 149-52
AB  - OBJECTIVE: To construct the eukaryotic expression vector of monocyte chemokine 
      protein 1 (MCP-1) driven by the cytomegalovirus (CMV) promoter, transfect the 
      vector into HEK293T cells, and detect its chemotaxis to macrophages. METHODS: 
      MCP-1 promoter was obtained from mouse genome by PCR, and inserted into the 
      vector named pFLAG-CMV1. We validated it by double-enzymes digestion and 
      sequencing. Then HEK293T cells were transfected with pFLAG-CMV1-MCP-1. The 
      expression of MCP-1 was detected by Western blotting. Finally, we identified the 
      chemotaxis of the recombinant vector to macrophage by Transwell(TM) assay. 
      RESULTS: The recombinant vector could generate target fragment by double enzyme 
      digestion. HEK293T cells expressed MCP-1 after they were transfected with the 
      recombinant vector, which increased the migration of macrophages. CONCLUSION: 
      MCP-1 expressed by HEK293T cells could apparently increase the migration of 
      macrophages.
FAU - Wang, Yuanyuan
AU  - Wang Y
AD  - Department of Nephrology, Xijing Hospital, Department of Medical Genetics and 
      Developmental Biology, School of Basic Medicine, Fourth Military Medical 
      University, Xi'an 710032, China.
FAU - Zhao, Junlong
AU  - Zhao J
AD  - Department of Medical Genetics and Developmental Biology, School of Basic 
      Medicine, Fourth Military Medical University, Xi'an 710032, China.
FAU - Liu, Juan
AU  - Liu J
AD  - Department of Medical Genetics and Developmental Biology, School of Basic 
      Medicine, Fourth Military Medical University, Xi'an 710032, China.
FAU - Jiang, Yali
AU  - Jiang Y
AD  - Department of Nephrology, Xijing Hospital, Department of Medical Genetics and 
      Developmental Biology, School of Basic Medicine, Fourth Military Medical 
      University, Xi'an 710032, China.
FAU - Han, Hua
AU  - Han H
AD  - Department of Medical Genetics and Developmental Biology, School of Basic 
      Medicine, Fourth Military Medical University, Xi'an 710032, China.
FAU - Qin, Hongyan
AU  - Qin H
AD  - Department of Medical Genetics and Developmental Biology, School of Basic 
      Medicine, Fourth Military Medical University, Xi'an 710032, China.
FAU - Liu, Xiaowei
AU  - Liu X
AD  - Department of Nephrology, Xijing Hospital, School of Basic Medicine, Fourth 
      Military Medical University, Xi'an 710032, China. *Corresponding author, E-mail: 
      liuxiaow@fmmu.edu.cn.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Chemokine CCL2)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Movement/*immunology
MH  - Chemokine CCL2/genetics/*immunology/metabolism
MH  - Cytomegalovirus/genetics
MH  - HEK293 Cells
MH  - Humans
MH  - Macrophages/cytology/*immunology
MH  - Mice
MH  - Promoter Regions, Genetic/genetics
MH  - Recombinant Fusion Proteins/genetics/*immunology/metabolism
MH  - Transfection
EDAT- 2016/03/02 06:00
MHDA- 2016/07/20 06:00
CRDT- 2016/03/02 06:00
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2016/07/20 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Feb;32(2):149-52.