PMID- 26927518
OWN - NLM
STAT- MEDLINE
DCOM- 20160812
LR  - 20211203
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 583
IP  - 1
DP  - 2016 May 25
TI  - Expression analyses of splice variants of zebrafish cyclin-dependent kinase-like 
      5 and its substrate, amphiphysin 1.
PG  - 15-23
LID - S0378-1119(16)30119-6 [pii]
LID - 10.1016/j.gene.2016.02.036 [doi]
AB  - Mammalian cyclin-dependent kinase-like 5 (CDKL5) is a Ser/Thr protein kinase 
      mainly expressed in the central nervous system and believed to be involved in 
      neuronal functions. However, the functions of CDKL5 in fishes have not been 
      investigated. Therefore, in this study, we cloned and characterized zebrafish 
      CDKL5 (zCDKL5) and its substrate, amphiphysin 1 (zAmph1). Two alternative splice 
      variants of zCDKL5, zCDKL5-Long (zCDKL5-L) and zCDKL5-Short (zCDKL5-S), and three 
      splice variants of zAmph1, zAmph1a, zAmph1b and zAmph1c, were cloned from a 
      zebrafish cDNA library. Using zAmph1a point mutants, we identified Ser-285 and 
      Ser-293 as phosphorylation sites of zAmph1a by CDKL5. Transiently expressed 
      zCDKL5-L and zCDKL5-S colocalized with zAmph1a in the cytoplasm of 293T cells. 
      RT-PCR analysis revealed that zCDKL5-L was first observed 12hours 
      post-fertilization (hpf) and increased thereafter, while zCDKL5-S appeared just 
      after fertilization. zAmph1a was detected in all embryogenic stages and zAmph1b 
      appeared from 12hpf, but the expression of zAmph1c was not observed in our 
      experiments. In adult fish, zCDKL5-L was mainly expressed in the brain, but 
      zCDKL5-S showed ubiquitous expression. zAmph1a was observed most abundantly in 
      the eyes, whereas zAmph1b was predominantly expressed in the brain. zAmph1c was 
      scarcely detected. These results suggest that phosphorylation of Amph1 by CDKL5 
      may be a common feature throughout animal species.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Katayama, Syouichi
AU  - Katayama S
AD  - Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 
      761-0795, Japan.
FAU - Senga, Yukako
AU  - Senga Y
AD  - Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 
      761-0795, Japan; Biomedical Research Institute, National Institute of Advanced 
      Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan.
FAU - Oi, Ami
AU  - Oi A
AD  - Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 
      761-0795, Japan.
FAU - Miki, Yosuke
AU  - Miki Y
AD  - Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 
      761-0795, Japan.
FAU - Sugiyama, Yasunori
AU  - Sugiyama Y
AD  - Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 
      761-0795, Japan.
FAU - Sueyoshi, Noriyuki
AU  - Sueyoshi N
AD  - Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 
      761-0795, Japan.
FAU - Kameshita, Isamu
AU  - Kameshita I
AD  - Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa 
      761-0795, Japan. Electronic address: kamesita@ag.kagawa-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160227
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 147954-52-7 (amphiphysin)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/metabolism
MH  - Cloning, Molecular
MH  - Cytoplasm/metabolism
MH  - Embryo, Nonmammalian
MH  - Gene Expression Regulation
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*genetics/metabolism
MH  - Phosphorylation
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Zebrafish/embryology/*genetics
MH  - Zebrafish Proteins/*genetics/metabolism
OTO - NOTNLM
OT  - Amphiphysin 1
OT  - Cyclin-dependent kinase-like 5
OT  - Expression analysis
OT  - Protein kinase
OT  - Splice variants
OT  - Zebrafish
EDAT- 2016/03/02 06:00
MHDA- 2016/08/16 06:00
CRDT- 2016/03/02 06:00
PHST- 2016/01/22 00:00 [received]
PHST- 2016/02/20 00:00 [revised]
PHST- 2016/02/22 00:00 [accepted]
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
AID - S0378-1119(16)30119-6 [pii]
AID - 10.1016/j.gene.2016.02.036 [doi]
PST - ppublish
SO  - Gene. 2016 May 25;583(1):15-23. doi: 10.1016/j.gene.2016.02.036. Epub 2016 Feb 
      27.