PMID- 26927552
OWN - NLM
STAT- MEDLINE
DCOM- 20160719
LR  - 20211203
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 32
IP  - 3
DP  - 2016 Mar
TI  - [Adipose-derived stem cells promote the polarization from M1 macrophages to M2 
      macrophages].
PG  - 332-8
AB  - OBJECTIVE: To investigate the effects of adipose-derived stem cells (ADSCs) on 
      M1/M2 macrophages and whether ADSCs are able to promote the polarization from M1 
      macrophages to M2 macrophages. METHODS: M1 macrophages were induced from J774.1 
      macrophages by 24-hour stimulation of lipopolysaccharide (LPS) and interferon gamma 
      (IFN-gamma), and M2 macrophages were induced from J774.1 macrophages by interleukin 4 
      (IL-4) for another 24 hours. Then M1/M2 macrophages were separately cultured in 
      the presence of ADSCs for 24 hours. The M1/M2 macrophages and their corresponding 
      supernatants were collected for further analysis. The expressions of IL-6, tumor 
      necrosis factor alpha (TNF-alpha), inducible nitric oxide synthase (iNOS), CC chemokine 
      ligand 2 (CCL2), CD86, arginase 1 (Arg1), mannose receptors/CD206 (MR/CD206), 
      IL-10, found in inflammatory zone 1 (FIZZ1), chitinase 3-like 3 (Ym-1) were 
      detected by real-time PCR and ELISA. RESULTS: ADSCs significantly decreased the 
      levels of IL-6, TNF-alpha, iNOS, CCL2 and CD86, and increased the levels of Arg1, 
      CD206 and IL-10 in M1 macrophages. In the supernatant of M1 macrophages, the 
      expressions of IL-6 and TNF-alpha were reduced, while those of CD206 were enhanced. 
      In M2 macrophages, ADSCs resulted in down-regulation of IL-6, TNF-alpha, iNOS, CD86 
      and up-regulation of Arg1, CD206, FIZZ-1, Ym-1 and IL-10. In the supernatant of 
      M2 macrophages, the expression levels of IL-6 and TNF-alpha were down-regulated and 
      those of CD206 were up-regulated. CONCLUSION: ADSCs can inhibit the gene 
      expression of M1 macrophages and promote the gene expression of M2 macrophages, 
      as well as mediate the polarization from M1 macrophages to M2 macrophages.
FAU - Yin, Xuehong
AU  - Yin X
AD  - First Affiliated Hospital, Baotou Medical College, Baotou 014040, China.
FAU - Pang, Chunyan
AU  - Pang C
AD  - First Affiliated Hospital, Baotou Medical College, Baotou 014040, China.
FAU - Bai, Li
AU  - Bai L
AD  - First Affiliated Hospital, Baotou Medical College, Baotou 014040, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - First Affiliated Hospital, Baotou Medical College, Baotou 014040, China.
FAU - Geng, Lixia
AU  - Geng L
AD  - First Affiliated Hospital, Baotou Medical College, Baotou 014040, China. 
      *Corresponding author, E-mail: genglixia8252@sina.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (B7-2 Antigen)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Lectins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Mannose Receptor)
RN  - 0 (Mannose-Binding Lectins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Retnla protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.2.1.52 (Chil3 protein, mouse)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - EC 3.5.3.1 (Arg1 protein, mouse)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Arginase/genetics/metabolism
MH  - B7-2 Antigen/genetics/metabolism
MH  - Cell Line
MH  - Chemokine CCL2/genetics/metabolism
MH  - Coculture Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-10/genetics/metabolism
MH  - Interleukin-4/pharmacology
MH  - Interleukin-6/genetics/metabolism
MH  - Lectins/genetics/metabolism
MH  - Lectins, C-Type/genetics/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - *Macrophage Activation
MH  - Macrophages/*cytology/drug effects/metabolism
MH  - Mannose Receptor
MH  - Mannose-Binding Lectins/genetics/metabolism
MH  - Mice, Inbred C57BL
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cells/*cytology
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - beta-N-Acetylhexosaminidases/genetics/metabolism
EDAT- 2016/03/02 06:00
MHDA- 2016/07/20 06:00
CRDT- 2016/03/02 06:00
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2016/07/20 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Mar;32(3):332-8.