PMID- 26927555
OWN - NLM
STAT- MEDLINE
DCOM- 20160719
LR  - 20171116
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 32
IP  - 3
DP  - 2016 Mar
TI  - [miR-126 knockdown enhances the activity of murine CD4+ T cells in vivo and 
      promotes their differentiation into Th1 cells].
PG  - 347-51
AB  - OBJECTIVE: To investigate the change of CD4(+) T cell activity in microRNA-126 
      (miR-126) knockdown (KD) mice and explore its significance. METHODS: The 
      expression level of mature miR-126 in CD4(+) CD62L(+) T cells purified by 
      magnetic-activated cell sorting (MACS) was analyzed by real-time PCR using 
      specific probe. Furthermore, the expression levels of CD69, CD62L and CD44 
      molecules, as well as intracellular proliferating nuclear antigen Ki-67, in 
      CD4(+) T cells in miR-126 KD mice were detected by fluorescence-activated cell 
      sorting (FACS). Moreover, the apoptosis of CD4(+) T cells was analyzed by annexin 
      V/PI staining assay combined with flow cytometry. Finally, the relative 
      expressions of function-related cytokines including interleukine 4 (IL-4), IL-10, 
      IL-12, transforming growth factor (TGF-beta), interferon (IFN-gamma) and tumor necrosis 
      factor (TNF-alpha) in CD4(+) T cells were determined by real-time PCR. RESULTS: 
      Compared with wild-type (WT) mice, the expression level of mature miR-126 in 
      CD4(+) T cells in miR-126 KD mice was dramatically reduced. Furthermore, the 
      proportion of CD62L(+) in CD4(+) T cells also decreased significantly, while the 
      proportions of CD69(+), CD44(+) and Ki-67(+) cells were remarkably elevated. 
      Meanwhile, the apoptosis proportion of CD4(+) T cells in vivo dropped 
      dramatically in miR-126 KD mice. Finally, the mRNA expressions of IL-4 and IL-10 
      in CD4(+)T cells were significantly downregulated, but IL-12, TGF-beta, TNF-alpha and 
      IFN-gamma mRNAs were obviously up-regulated. CONCLUSION: miR-126 knockdown could 
      significantly enhance the functional activity of CD4(+) T cells in vivo and 
      promote cell differentiation into Th1 cells.
FAU - Cui, Panpan
AU  - Cui P
AD  - Department of Immunology, Zunyi Medical College, Zunyi 563000, China.
FAU - Hu, Yan
AU  - Hu Y
AD  - Department of Immunology, Zunyi Medical College, Zunyi 563000, China.
FAU - Tao, Yijing
AU  - Tao Y
AD  - Department of Immunology, Zunyi Medical College, Zunyi 563000, China.
FAU - Chen, Chao
AU  - Chen C
AD  - Department of Immunology, Zunyi Medical College, Zunyi 563000, China.
FAU - Zhao, Juanjuan
AU  - Zhao J
AD  - Department of Immunology, Zunyi Medical College, Zunyi 563000, China.
FAU - Guo, Mengmeng
AU  - Guo M
AD  - Department of Immunology, Zunyi Medical College, Zunyi 563000, China.
FAU - Zhou, Ya
AU  - Zhou Y
AD  - Department of Medical Physics, Zunyi Medical College, Zunyi 563000, China.
FAU - Xu, Lin
AU  - Xu L
AD  - Department of Immunology, Zunyi Medical College, Zunyi 563000, China. 
      *Corresponding author, E-mail: xulinzhouya@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD69 antigen)
RN  - 0 (Cytokines)
RN  - 0 (Hyaluronan Receptors)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Lectins, C-Type)
RN  - 0 (MIRN126 microRNA, mouse)
RN  - 0 (MicroRNAs)
RN  - 126880-86-2 (L-Selectin)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, T-Lymphocyte/metabolism
MH  - CD4-Positive T-Lymphocytes/cytology/immunology/*metabolism
MH  - Cell Differentiation/*genetics/immunology
MH  - Cytokines/genetics
MH  - Flow Cytometry
MH  - Gene Expression
MH  - Gene Knockdown Techniques/*methods
MH  - Hyaluronan Receptors/metabolism
MH  - Immunomagnetic Separation
MH  - Ki-67 Antigen/metabolism
MH  - L-Selectin/metabolism
MH  - Lectins, C-Type/metabolism
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Th1 Cells/cytology/immunology/*metabolism
EDAT- 2016/03/02 06:00
MHDA- 2016/07/20 06:00
CRDT- 2016/03/02 06:00
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2016/07/20 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Mar;32(3):347-51.