PMID- 26927991
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 2212-554X (Electronic)
IS  - 2212-5531 (Linking)
VI  - 5
IP  - 1
DP  - 2016 Mar
TI  - Molecular epidemiology of Mycobacterium avium subspecies paratuberculosis in 
      ruminants in different parts of India.
PG  - 59-65
LID - S2212-5531(15)00165-X [pii]
LID - 10.1016/j.ijmyco.2015.11.003 [doi]
AB  - OBJECTIVE/BACKGROUND: Paratuberculosis is an economically important, chronic, and 
      incurable disease in ruminants, caused by Mycobacterium avium subspecies 
      paratuberculosis (MAP). Understanding the genetic variability of MAP strains is 
      important in diagnosis, epidemiological investigation, and the formation of 
      strategies for prevention and control of the disease. METHODS: In the present 
      study, a total of 61 MAP isolates obtained from different parts and species of 
      India were typed using IS1311 polymerase chain reaction-restriction endonuclease 
      analysis (PCR-REA) to analyze the genetic difference(s), if any, between them and 
      the host adaptation. RESULTS: Based on PCR-REA results, bison B type was detected 
      in 54 (87%) MAP isolates obtained from cattle, sheep, and goats. Of these, 19 
      were from sheep of the Rajasthan (n=17) and Bareilly (n=2), North India regions, 
      28 were from cattle of Chennai, South India (n=3), Bareilly, North India (n=3), 
      and Nagpur, West India (n=22), and seven goat isolates from Bareilly, North India 
      region. The 'C' type strain was detected in only seven cattle isolates obtained 
      from the Bareilly region. CONCLUSION: The study revealed that in India, bison 
      B-type MAP strains were prevalent in most of the ruminant species. These results 
      have important epidemiological implications with regard to control and prevention 
      of paratuberculosis in India.
CI  - Copyright (c) 2015 Asian African Society for Mycobacteriology. Published by 
      Elsevier Ltd. All rights reserved.
FAU - Sonawane, Ganesh G
AU  - Sonawane GG
AD  - Division of Pathology, ICAR-IVRI, Izatnagar, Bareilly, India. Electronic address: 
      sganesh413@gmail.com.
FAU - Narnaware, Shirish D
AU  - Narnaware SD
AD  - Division of Pathology, ICAR-IVRI, Izatnagar, Bareilly, India.
FAU - Tripathi, Bhupendra N
AU  - Tripathi BN
AD  - Division of Pathology, ICAR-IVRI, Izatnagar, Bareilly, India.
LA  - eng
PT  - Journal Article
DEP - 20151228
PL  - India
TA  - Int J Mycobacteriol
JT  - International journal of mycobacteriology
JID - 101615660
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - Bison/microbiology
MH  - Cattle/microbiology
MH  - DNA, Bacterial/genetics
MH  - Goats/microbiology
MH  - India/epidemiology
MH  - Molecular Epidemiology
MH  - Mycobacterium avium subsp. paratuberculosis/*genetics
MH  - Paratuberculosis/*epidemiology/*microbiology
MH  - Polymerase Chain Reaction
MH  - Restriction Mapping
MH  - Ruminants/*microbiology
MH  - Sheep/microbiology
MH  - Sheep, Domestic/microbiology
OTO - NOTNLM
OT  - India
OT  - Molecular typing
OT  - Mycobacteium avium subspecies paratuberculosis
OT  - Ruminants
EDAT- 2016/03/02 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/03/02 06:00
PHST- 2015/10/07 00:00 [received]
PHST- 2015/11/24 00:00 [revised]
PHST- 2015/11/28 00:00 [accepted]
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S2212-5531(15)00165-X [pii]
AID - 10.1016/j.ijmyco.2015.11.003 [doi]
PST - ppublish
SO  - Int J Mycobacteriol. 2016 Mar;5(1):59-65. doi: 10.1016/j.ijmyco.2015.11.003. Epub 
      2015 Dec 28.