PMID- 26928722
OWN - NLM
STAT- MEDLINE
DCOM- 20161107
LR  - 20240324
IS  - 1756-0500 (Electronic)
IS  - 1756-0500 (Linking)
VI  - 9
DP  - 2016 Feb 29
TI  - Unbiased profiling of volatile organic compounds in the headspace of Allium 
      plants using an in-tube extraction device.
PG  - 133
LID - 10.1186/s13104-016-1942-5 [doi]
LID - 133
AB  - BACKGROUND: Plants produce and emit important volatile organic compounds (VOCs), 
      which have an essential role in biotic and abiotic stress responses and in 
      plant-plant and plant-insect interactions. In order to study the bouquets from 
      plants qualitatively and quantitatively, a comprehensive, analytical method 
      yielding reproducible results is required. RESULTS: We applied in-tube extraction 
      (ITEX) and solid-phase microextraction (SPME) for studying the emissions of 
      Allium plants. The collected HS samples were analyzed by gas 
      chromatography-time-of-flight-mass spectrometry (GC-TOF-MS), and the results were 
      subjected to multivariate analysis. In case of ITEX-method Allium cultivars 
      released more than 300 VOCs, out of which we provisionally identified 50 
      volatiles. We also used the VOC profiles of Allium samples to discriminate among 
      groups of A. fistulosum, A. chinense (rakkyo), and A. tuberosum (Oriental 
      garlic). As we found 12 metabolite peaks including dipropyl disulphide with 
      significant changes in A. chinense and A. tuberosum when compared to the control 
      cultivar, these metabolite peaks can be used for chemotaxonomic classification of 
      A. chinense, tuberosum, and A. fistulosum. CONCLUSIONS: Compared to SPME-method 
      our ITEX-based VOC profiling technique contributes to automatic and reproducible 
      analyses. Hence, it can be applied to high-throughput analyses such as metabolite 
      profiling.
FAU - Kusano, Miyako
AU  - Kusano M
AD  - Graduate School of Life and Environmental Sciences, University of Tsukuba, 
      Tennodai, Tsukuba, Ibaraki, Japan. kusano.miyako.fp@u.tsukuba.ac.jp.
AD  - RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. 
      kusano.miyako.fp@u.tsukuba.ac.jp.
FAU - Kobayashi, Makoto
AU  - Kobayashi M
AD  - RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. 
      kobamako@riken.jp.
FAU - Iizuka, Yumiko
AU  - Iizuka Y
AD  - RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. 
      yiizuka5@gmail.com.
AD  - Department of Genome System Science, Graduate School of Nanobioscience, Yokohama 
      City University, Yokohama, Kanagawa, Japan. yiizuka5@gmail.com.
FAU - Fukushima, Atsushi
AU  - Fukushima A
AD  - RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. 
      atsushi.fukushima@riken.jp.
FAU - Saito, Kazuki
AU  - Saito K
AD  - RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan. 
      kazuki.saito@riken.jp.
AD  - Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Chiba, 
      Japan. kazuki.saito@riken.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160229
PL  - England
TA  - BMC Res Notes
JT  - BMC research notes
JID - 101462768
RN  - 0 (Plant Extracts)
RN  - 0 (Volatile Organic Compounds)
SB  - IM
MH  - Allium/chemistry/*classification/*metabolism
MH  - Gas Chromatography-Mass Spectrometry
MH  - Metabolome/*physiology
MH  - Plant Components, Aerial/chemistry
MH  - Plant Extracts/chemistry
MH  - Solid Phase Microextraction/*instrumentation/methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Volatile Organic Compounds/classification/*isolation & purification
PMC - PMC4772445
EDAT- 2016/03/02 06:00
MHDA- 2016/11/08 06:00
PMCR- 2016/02/29
CRDT- 2016/03/02 06:00
PHST- 2015/08/23 00:00 [received]
PHST- 2016/02/17 00:00 [accepted]
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2016/11/08 06:00 [medline]
PHST- 2016/02/29 00:00 [pmc-release]
AID - 10.1186/s13104-016-1942-5 [pii]
AID - 1942 [pii]
AID - 10.1186/s13104-016-1942-5 [doi]
PST - epublish
SO  - BMC Res Notes. 2016 Feb 29;9:133. doi: 10.1186/s13104-016-1942-5.