PMID- 26929401
OWN - NLM
STAT- MEDLINE
DCOM- 20170608
LR  - 20201209
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 473
IP  - 9
DP  - 2016 May 1
TI  - Identification of ferrichrome- and ferrioxamine B-mediated iron uptake by 
      Aspergillus fumigatus.
PG  - 1203-13
LID - 10.1042/BCJ20160066 [doi]
AB  - Aspergillus fumigatus is an opportunistic fungal pathogen for immunocompromised 
      patients, and genes involved in siderophore metabolism have been identified as 
      virulence factors. Recently, we identified the membrane transporters sit1 and 
      sit2, which are putative virulence factors of A. fumigatus; sit1 and sit2 are 
      homologous to yeast Sit1, and sit1 and sit2 gene expression was up-regulated 
      after iron depletion. When expressed heterologously in Saccharomyces cerevisiae, 
      sit1 and sit2 were localized to the plasma membrane; sit1 efficiently 
      complemented ferrichrome (FC) and ferrioxamine B (FOB) uptake in yeast cells, 
      whereas sit2 complemented only FC uptake. Deletion of sit1 resulted in a decrease 
      in FOB and FC uptake, and deletion of sit2 resulted in a decrease in FC uptake in 
      A. fumigatus It is of interest that a sit1 and sit2 double-deletion mutant 
      resulted in a synergistic decrease in FC uptake activity. Both sit1 and sit2 were 
      localized to the plasma membrane in A. fumigatus The expression levels of the 
      sit1 and sit2 genes were dependent on hapX under low-but not high-iron 
      conditions. Furthermore, mirB, and sidA gene expression was up-regulated and sreA 
      expression down-regulated when sit1 and sit2 were deleted. Although sit1 and sit2 
      failed to affect mouse survival rate, these genes affected conidial killing 
      activity. Taken together, our results suggest that sit1 and sit2 are siderophore 
      transporters and putative virulence factors localized to the plasma membrane.
CI  - (c) 2016 The Author(s). published by Portland Press Limited on behalf of the 
      Biochemical Society.
FAU - Park, Yong-Sung
AU  - Park YS
AD  - School of Life Sciences and Biotechnology, Korea University Anam-dong, 
      Sungbuk-gu, Seoul, Republic of Korea.
FAU - Kim, Ju-Yeon
AU  - Kim JY
AD  - School of Life Sciences and Biotechnology, Korea University Anam-dong, 
      Sungbuk-gu, Seoul, Republic of Korea.
FAU - Yun, Cheol-Won
AU  - Yun CW
AD  - School of Life Sciences and Biotechnology, Korea University Anam-dong, 
      Sungbuk-gu, Seoul, Republic of Korea cheolwony@korea.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20160229
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Ferric Compounds)
RN  - 0 (Fungal Proteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Virulence Factors)
RN  - 15630-64-5 (Ferrichrome)
RN  - 4A0UG9NR9K (ferrioxamine B)
RN  - E1UOL152H7 (Iron)
RN  - J06Y7MXW4D (Deferoxamine)
SB  - IM
MH  - Animals
MH  - Aspergillus fumigatus/genetics/*metabolism/pathogenicity
MH  - Cell Membrane/genetics/*metabolism
MH  - Deferoxamine/*metabolism
MH  - Ferric Compounds/*metabolism
MH  - Ferrichrome/*metabolism
MH  - Fungal Proteins/genetics/metabolism
MH  - Iron/*metabolism
MH  - Membrane Transport Proteins/genetics/metabolism
MH  - Mice
MH  - Virulence Factors/genetics/*metabolism
OTO - NOTNLM
OT  - Aspergillus fumigatus
OT  - Saccharomyces cerevisiae
OT  - iron
OT  - siderophore
OT  - sit1
OT  - sit2
EDAT- 2016/03/02 06:00
MHDA- 2017/06/09 06:00
CRDT- 2016/03/02 06:00
PHST- 2015/12/04 00:00 [received]
PHST- 2016/02/26 00:00 [accepted]
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2017/06/09 06:00 [medline]
AID - BCJ20160066 [pii]
AID - 10.1042/BCJ20160066 [doi]
PST - ppublish
SO  - Biochem J. 2016 May 1;473(9):1203-13. doi: 10.1042/BCJ20160066. Epub 2016 Feb 29.