PMID- 26929585
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160301
LR  - 20200929
IS  - 0973-1296 (Print)
IS  - 0976-4062 (Electronic)
IS  - 0973-1296 (Linking)
VI  - 11
IP  - Suppl 3
DP  - 2015 Oct
TI  - Selective Cytotoxicity and Pro-apoptotic Activity of Stem Bark of Wrightia 
      tinctoria (Roxb.) R. Br. in Cancerous Cells.
PG  - S481-7
LID - 10.4103/0973-1296.168976 [doi]
AB  - BACKGROUND: Wrightia tinctoria (Roxb.) R. Br. is a widely available shrub in 
      India used traditionally in various ailments, including cancer. However, the 
      anticancer activity of the bioactive fractions has not been validated 
      scientifically. OBJECTIVE: To investigate the anticancer potential of stem bark 
      of W. tinctoria and establish its phytochemical basis. MATERIALS AND METHODS: The 
      ethanol extract and subsequent fractions, petroleum ether, ethyl acetate, 
      n-butanol, and aqueous were prepared by standard methods. In vitro cytotoxicity 
      was determined in MCF-7 (breast) and HeLa (cervical) adenocarcinoma cells, and 
      V79 (nontumor fibroblast) cells and apoptogenic activity in MCF-7 cells by 
      acridine orange (AO)/ethidium bromide (EB) staining. Additionally, the 
      antioxidant potential was evaluated using suitable methods. High-performance thin 
      layer chromatography (HPTLC) analysis was performed for identification of active 
      phytoconstituents. RESULTS: Petroleum ether and ethyl acetate fractions were most 
      potent with IC50 values of 37.78 and 29.69 mug/ml in HeLa and 31.56 and 32.63 
      mug/ml in MCF-7 cells respectively in the sulforhodamine B assay. Comparable 
      results were obtained in HeLa cells in 
      3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyl tetrazolium bromide assay and 
      interestingly, the fractions were found to be safe to noncancerous fibroblast 
      cells. Both fractions induced significant (P < 0.05) apoptotic morphological 
      changes observed by AO/EB staining. Moreover, extract/fractions exhibited 
      excellent inhibition of lipid peroxidation with the ethyl acetate fraction being 
      most active (IC50:23.40 mug/ml). HPTLC confirmed the presence of two anti-cancer 
      triterpenoids, lupeol, and beta-sitosterol in active fractions. CONCLUSION: 
      Extract/fractions of W. tinctoria exhibit selective cytotoxicity against 
      cancerous cells that is mediated by apoptosis. Fractions are less toxic to 
      noncancerous cells; hence, they can be developed as safer chemopreventive agents. 
      SUMMARY: Petroleum ether and ethyl acetate fractions were most active and 
      exhibited dose-dependent cytotoxicity in HeLa and MCF-7 cells.Fractions were 
      relatively less toxic to non-tumor fibroblast cells demonstrating its selectivity 
      to cancer cells.Fractions exhibited pro-apoptotic activity in MCF-7 cells in 
      AO/EB staining.Lupeol and beta-sitosterol were identified as anticancer constituents 
      by HPTLC.
FAU - Chaudhary, Shilpee
AU  - Chaudhary S
AD  - Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal 
      University, Manipal, Karnataka, India.
FAU - Devkar, Raviraj Anand
AU  - Devkar RA
AD  - Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 
      University, Manipal, Karnataka, India.
FAU - Bhere, Deepak
AU  - Bhere D
AD  - Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal 
      University, Manipal, Karnataka, India; Department of Radiology, Massachusetts 
      General Hospital, Harvard Medical School, Boston, MA 02114, USA.
FAU - Setty, Manganahalli Manjunath
AU  - Setty MM
AD  - Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 
      University, Manipal, Karnataka, India.
FAU - Pai, Karkala Sreedhara Ranganath
AU  - Pai KS
AD  - Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal 
      University, Manipal, Karnataka, India.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pharmacogn Mag
JT  - Pharmacognosy magazine
JID - 101300403
PMC - PMC4745221
OTO - NOTNLM
OT  - anticancer
OT  - chemoprevention
OT  - high-performance thin layer chromatography
OT  - lupeol
OT  - beta-sitosterol
EDAT- 2016/03/02 06:00
MHDA- 2016/03/02 06:01
PMCR- 2015/10/01
CRDT- 2016/03/02 06:00
PHST- 2016/03/02 06:00 [entrez]
PHST- 2016/03/02 06:00 [pubmed]
PHST- 2016/03/02 06:01 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - PM-11-481 [pii]
AID - 10.4103/0973-1296.168976 [doi]
PST - ppublish
SO  - Pharmacogn Mag. 2015 Oct;11(Suppl 3):S481-7. doi: 10.4103/0973-1296.168976.