PMID- 26930960
OWN - NLM
STAT- MEDLINE
DCOM- 20160426
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 10
DP  - 2015 Oct
TI  - [Determination of fructo-oligosaccharides in milk powder by high performance 
      liquid chromatography-quadrupole/electrostatic field orbitrap high resolution 
      mass spectrometry].
PG  - 1040-5
AB  - A method of high performance liquid chromatography-quadrupole/electrostatic field 
      Orbitrap high resolution mass spectrometry (HPLC-Q/Orbitrap MS) was developed to 
      determine fructo-oligosaccharides in milk powder. The milk powder samples were 
      dissolved in deionized water. Subsequently, an aqueous solution of zinc acetate 
      was used to precipitate protein. After centrifugation, the final aqueous solution 
      was filtered by a polytetrafluoroethylene (PTFE) membrane with pore size of 0.22 
      mum. The analytes were separated on a Carbohydrate column (100 mm x 2.1 mm, 2.6 
      mum) through gradient elution with the combination of acetonitrile and 0.1% formic 
      acid aqueous solution. The target-MS/MS templates were performed at isolation 
      window of m/z 4.0 and collision energy of 30 eV in positive mode to extract the 
      accurate product ion mass of analytes. Under the optimal condition, 1-kestose 
      (GF2), nystose (GF3) and 1-F-beta-fructofuranosyl nystose (GF4) were well separated 
      and the accuracy of extracted mass routinely detected was below 5 x 10(-6) (5 
      ppm). The whole analysis time is only ten minutes. The detection limits for GF2 
      and GF3 were 100 mug/kg, and the detection limit for GF4 was 55 mug/kg. Good 
      linearities were obtained in their respective linear ranges with correlation 
      coefficients higher than 0.998. The average recoveries at three spiked levels (5, 
      10 and 20 mg/kg) were in the range of 75.8%-107.3% and the relative standard 
      deviations (RSDs) were in the range of 1.6% - 8.3%. The proposed method is 
      simple, sensitive, fast and only in need of precipitation of proteins. The 
      interference of matrix can be eliminated through the selection of product ion. 
      The results were convenient and reliable and thus can be used in the large batch 
      determination of any milk powder.
FAU - Liu, Yun
AU  - Liu Y
FAU - Ding, Tao
AU  - Ding T
FAU - Xu, Suli
AU  - Xu S
FAU - Wu, Bin
AU  - Wu B
FAU - Shen, Chongyu
AU  - Shen C
FAU - Zhang, Rui
AU  - Zhang R
FAU - Wang, Yan
AU  - Wang Y
FAU - Fei, Xiaoqing
AU  - Fei X
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Oligosaccharides)
RN  - 0 (fructooligosaccharide)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Milk/*chemistry
MH  - Oligosaccharides/*analysis
MH  - Tandem Mass Spectrometry
EDAT- 2016/03/05 06:00
MHDA- 2016/04/27 06:00
CRDT- 2016/03/03 06:00
PHST- 2016/03/03 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2016/04/27 06:00 [medline]
AID - 10.3724/sp.j.1123.2015.06010 [doi]
PST - ppublish
SO  - Se Pu. 2015 Oct;33(10):1040-5. doi: 10.3724/sp.j.1123.2015.06010.