PMID- 26932855
OWN - NLM
STAT- MEDLINE
DCOM- 20170317
LR  - 20181202
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 96
IP  - 8
DP  - 2016 Mar 1
TI  - [GDF11 level in patients with myelodysplastic syndrome and its clinical 
      significance].
PG  - 620-4
LID - 10.3760/cma.j.issn.0376-2491.2016.08.008 [doi]
AB  - OBJECTIVE: To detect the expression level of growth differentiation factor 11 
      (GDF11) in patients with myelodysplastic syndrome (MDS), and to evaluate the 
      relationship between GDF11 level and erythropoiesis functions. METHODS: A total 
      of 44 MDS patients (18 low-risk group patients and 26 high-risk group patients) 
      in Department of Hematology in Tianjin Medical University General Hospital and 10 
      normal controls were selected from September 2014 to June 2015. The concentration 
      of GDF11 in peripheral blood was detected using enzyme-linked immunosorbent assay 
      (ELISA). GDF11 mRNA expression in bone marrow mononuclear cells (BMMNC) was 
      detected using RT-PCR method. The percentage of erythroid cells (CD235a) in bone 
      marrow was detected by flow cytometry. The correlation between these indexes and 
      erythropoiesis functions (including red blood cell count (RBC), hemoglobin level 
      (Hb), hematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular 
      hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), reticulocyte 
      percentage (Ret%), and proportion of nucleated eryhrocyte in bone marrow) were 
      evaluated. RESULTS: (1) The GDF11 level in peripheral blood was significantly 
      higher in high-risk group ((128.67+/-47.62) mug/L) than in low-risk group 
      ((65.96+/-36.55) mug/L, P<0.01) and in normal controls ((29.76+/-10.10) mug/L, P<0.01), 
      also significantly higher in low-risk group than in normal controls (P<0.05). The 
      concentration of GDF11 in severe/moderate anemic MDS patients ((80.97+/-9.94) mug/L) 
      was higher than that in normal controls/ mild anemic MDS patients((66.82+/-19.52) 
      mug/L), but with no statistically significant (P>0.05). (2) The percentages of 
      CD235a(+) cells in high-risk and low-risk groups were 38.49%+/-5.42% and 
      42.64%+/-7.36%, respectively, showing no statistically significant difference 
      (P>0.05). (3) In high-risk group, the GDF11 level in peripheral blood was 
      negatively correlated with Hb, RBC and Hct in peripheral blood (r=-0.437, -0.430, 
      -0.306, all P<0.05), and positively correlated with nucleated eryhrocyte, Ret%, 
      MCV and CD235a(+) cells in bone marrow (r=0.465, 0.392, 0.505, 0.387, all 
      P<0.05), but not correlated with MCH and MCHC (both P>0.05). In low-risk group, 
      the GDF11 level in peripheral blood was positively correlated with CD235a(+) 
      cells in bone marrow (r=0.429, P<0.05), and not correlated with Hb, RBC, Ret%, 
      MCHC, MCV, MCH, Hct and nucleated eryhrocyte (all P>0.05). (4) The mRNA 
      expression of GDF11 in MDS patients (39.82+/-14.55) was higher than that in the 
      controls (1.84+/-0.64, P<0.01). CONCLUSIONS: GDF11 level in peripheral blood is 
      higher in patients with MDS and increases with the disease risk. The more severe 
      the anemia, the higher the GDF11 level. It may be closely correlated with 
      erythropoiesis indicators in MDS.
FAU - Han, Yu
AU  - Han Y
AD  - Department of Hematology, Tianjin Medical University General Hospital, Tianjin 
      300052, China.
FAU - Wang, Huaquan
AU  - Wang H
FAU - Fu, Rong
AU  - Fu R
FAU - Qu, Wen
AU  - Qu W
FAU - Ruan, Erbao
AU  - Ruan E
FAU - Wang, Xiaoming
AU  - Wang X
FAU - Wang, Guojin
AU  - Wang G
FAU - Wu, Yuhong
AU  - Wu Y
FAU - Liu, Hong
AU  - Liu H
FAU - Song, Jia
AU  - Song J
FAU - Guan, Jing
AU  - Guan J
FAU - Xing, Limin
AU  - Xing L
FAU - Li, Lijuan
AU  - Li L
FAU - Jiang, Huijuan
AU  - Jiang H
FAU - Liu, Hui
AU  - Liu H
FAU - Wang, Yihao
AU  - Wang Y
FAU - Liu, Chunyan
AU  - Liu C
FAU - Zhang, Wei
AU  - Zhang W
FAU - Shao, Zonghong
AU  - Shao Z
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (GDF11 protein, human)
RN  - 0 (Growth Differentiation Factors)
SB  - IM
MH  - Anemia
MH  - Bone Marrow
MH  - Bone Marrow Cells
MH  - Bone Morphogenetic Proteins
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Erythrocyte Indices
MH  - Flow Cytometry
MH  - Growth Differentiation Factors
MH  - Humans
MH  - *Myelodysplastic Syndromes
EDAT- 2016/03/05 06:00
MHDA- 2017/03/18 06:00
CRDT- 2016/03/03 06:00
PHST- 2016/03/03 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2017/03/18 06:00 [medline]
AID - 10.3760/cma.j.issn.0376-2491.2016.08.008 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2016 Mar 1;96(8):620-4. doi: 
      10.3760/cma.j.issn.0376-2491.2016.08.008.