PMID- 26935842
OWN - NLM
STAT- MEDLINE
DCOM- 20170615
LR  - 20181113
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 78
IP  - 6
DP  - 2016 Jul 1
TI  - Establishment of serological test to detect antibody against ferret coronavirus.
PG  - 1013-7
LID - 10.1292/jvms.16-0059 [doi]
AB  - Since there is no available serological methods to detect antibodies to ferret 
      coronavirus (FRCoV), an enzyme-linked immunosorbent assay (ELISA) using 
      recombinant partial nucleocapsid (N) proteins of the ferret coronavirus (FRCoV) 
      Yamaguchi-1 strain was developed to establish a serological method for detection 
      of FRCoV infection. Many serum samples collected from ferrets recognized both 
      a.a. 1-179 and a.a. 180-374 of the N protein, but two serum samples did not a.a. 
      180-374 of the N protein. This different reactivity was also confirmed by 
      immunoblot analysis using the serum from the ferret.Therefore, the a.a. 1-179 of 
      the N protein was used as an ELISA antigen. Serological test was carried out 
      using sera or plasma of ferrets in Japan. Surprisingly, 89% ferrets in Japan had 
      been infected with FRCoV. These results indicated that our established ELISA 
      using a.a. 1-179 of the N protein is useful for detection of antibody to FRCoV 
      for diagnosis and seroepidemiology of FRCoV infection.
FAU - Minami, Shohei
AU  - Minami S
AD  - Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, 
      Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan.
FAU - Terada, Yutaka
AU  - Terada Y
FAU - Shimoda, Hiroshi
AU  - Shimoda H
FAU - Takizawa, Masaki
AU  - Takizawa M
FAU - Onuma, Mamoru
AU  - Onuma M
FAU - Ota, Akihiko
AU  - Ota A
FAU - Ota, Yuichi
AU  - Ota Y
FAU - Akabane, Yoshihito
AU  - Akabane Y
FAU - Tamukai, Kenichi
AU  - Tamukai K
FAU - Watanabe, Keiichiro
AU  - Watanabe K
FAU - Naganuma, Yumiko
AU  - Naganuma Y
FAU - Kanagawa, Eiichi
AU  - Kanagawa E
FAU - Nakamura, Kaneichi
AU  - Nakamura K
FAU - Ohashi, Masanari
AU  - Ohashi M
FAU - Takami, Yoshinori
AU  - Takami Y
FAU - Miwa, Yasutsugu
AU  - Miwa Y
FAU - Tanoue, Tomoaki
AU  - Tanoue T
FAU - Ohwaki, Masao
AU  - Ohwaki M
FAU - Ohta, Jouji
AU  - Ohta J
FAU - Une, Yumi
AU  - Une Y
FAU - Maeda, Ken
AU  - Maeda K
LA  - eng
PT  - Journal Article
DEP - 20160303
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*immunology
MH  - Antigens, Viral/immunology
MH  - Coronavirus/*immunology
MH  - Coronavirus Infections/diagnosis/immunology/veterinary/virology
MH  - Enzyme-Linked Immunosorbent Assay/methods/veterinary
MH  - Female
MH  - Ferrets/*virology
MH  - Immunoblotting/veterinary
MH  - Male
PMC - PMC4937135
EDAT- 2016/03/05 06:00
MHDA- 2017/06/16 06:00
PMCR- 2016/06/01
CRDT- 2016/03/04 06:00
PHST- 2016/03/04 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2017/06/16 06:00 [medline]
PHST- 2016/06/01 00:00 [pmc-release]
AID - 16-0059 [pii]
AID - 10.1292/jvms.16-0059 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2016 Jul 1;78(6):1013-7. doi: 10.1292/jvms.16-0059. Epub 2016 Mar 
      3.