PMID- 26935918
OWN - NLM
STAT- MEDLINE
DCOM- 20170130
LR  - 20200325
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 161
IP  - 6
DP  - 2016 Jun
TI  - Analysis of the entry mechanism of Crimean-Congo hemorrhagic fever virus, using a 
      vesicular stomatitis virus pseudotyping system.
PG  - 1447-54
LID - 10.1007/s00705-016-2803-1 [doi]
AB  - Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease causing severe 
      hemorrhagic symptoms with a nearly 30 % case-fatality rate in humans. The 
      experimental use of CCHF virus (CCHFV), which causes CCHF, requires 
      high-biosafety-level (BSL) containment. In contrast, pseudotyping of various 
      viral glycoproteins (GPs) onto vesicular stomatitis virus (VSV) can be used in 
      facilities with lower BSL containment, and this has facilitated studies on the 
      viral entry mechanism and the measurement of neutralizing activity, especially 
      for highly pathogenic viruses. In the present study, we generated high titers of 
      pseudotyped VSV bearing the CCHFV envelope GP and analyzed the mechanisms 
      involved in CCHFV infection. A partial deletion of the CCHFV GP cytoplasmic 
      domain increased the titer of the pseudotyped VSV, the entry mechanism of which 
      was dependent on the CCHFV envelope GP. Using the pseudotype virus, DC-SIGN (a 
      calcium-dependent [C-type] lectin cell-surface molecule) was revealed to enhance 
      viral infection and act as an entry factor for CCHFV.
FAU - Suda, Yuto
AU  - Suda Y
AD  - Department of Veterinary Microbiology, Graduate School of Agricultural and Life 
      Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, 
      Japan.
AD  - Special Pathogens Laboratory, Department of Virology I, National Institute of 
      Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo, 208-0011, Japan.
FAU - Fukushi, Shuetsu
AU  - Fukushi S
AD  - Special Pathogens Laboratory, Department of Virology I, National Institute of 
      Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo, 208-0011, Japan.
FAU - Tani, Hideki
AU  - Tani H
AD  - Special Pathogens Laboratory, Department of Virology I, National Institute of 
      Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo, 208-0011, Japan.
FAU - Murakami, Shin
AU  - Murakami S
AD  - Department of Veterinary Microbiology, Graduate School of Agricultural and Life 
      Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, 
      Japan.
FAU - Saijo, Masayuki
AU  - Saijo M
AD  - Special Pathogens Laboratory, Department of Virology I, National Institute of 
      Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo, 208-0011, Japan.
FAU - Horimoto, Taisuke
AU  - Horimoto T
AD  - Department of Veterinary Microbiology, Graduate School of Agricultural and Life 
      Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, 
      Japan.
FAU - Shimojima, Masayuki
AU  - Shimojima M
AD  - Special Pathogens Laboratory, Department of Virology I, National Institute of 
      Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo, 208-0011, Japan. 
      shimoji-@nih.go.jp.
LA  - eng
PT  - Journal Article
DEP - 20160303
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Cell Adhesion Molecules/physiology
MH  - Chlorocebus aethiops
MH  - HEK293 Cells
MH  - Hemorrhagic Fever Virus, Crimean-Congo/genetics/pathogenicity/*physiology
MH  - Hemorrhagic Fever, Crimean/etiology/virology
MH  - Humans
MH  - Jurkat Cells
MH  - Lectins, C-Type/physiology
MH  - Receptors, Cell Surface/physiology
MH  - Vero Cells
MH  - Vesicular stomatitis Indiana virus/genetics/physiology
MH  - Viral Envelope Proteins/genetics/physiology
MH  - *Virus Internalization
PMC - PMC7087235
EDAT- 2016/03/05 06:00
MHDA- 2017/01/31 06:00
PMCR- 2020/03/23
CRDT- 2016/03/04 06:00
PHST- 2015/09/24 00:00 [received]
PHST- 2016/02/21 00:00 [accepted]
PHST- 2016/03/04 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2017/01/31 06:00 [medline]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 10.1007/s00705-016-2803-1 [pii]
AID - 2803 [pii]
AID - 10.1007/s00705-016-2803-1 [doi]
PST - ppublish
SO  - Arch Virol. 2016 Jun;161(6):1447-54. doi: 10.1007/s00705-016-2803-1. Epub 2016 
      Mar 3.