PMID- 26937219
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160303
LR  - 20200928
IS  - 1226-4512 (Print)
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Linking)
VI  - 20
IP  - 2
DP  - 2016 Mar
TI  - Effects of prunetin on the proteolytic activity, secretion and gene expression of 
      MMP-3 in vitro and production of MMP-3 in vivo.
PG  - 221-8
LID - 10.4196/kjpp.2016.20.2.221 [doi]
AB  - We investigated whether prunetin affects the proteolytic activity, secretion, and 
      gene expression of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit 
      articular chondrocytes, as well as in vivo production of MMP-3 in the rat knee 
      joint to evaluate the potential chondroprotective eff ect of prunetin. Rabbit 
      articular chondrocytes were cultured in a monolayer, and reverse 
      transcription-polymerase chain reaction (RT-PCR) was used to measure 
      interleukin-1beta (IL-1beta)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin 
      and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and ADAMTS-5. In 
      rabbit articular chondrocytes, the effects of prunetin on IL-1beta-induced secretion 
      and proteolytic activity of MMP-3 were investigated using western blot analysis 
      and casein zymography, respectively. The eff ect of prunetin on MMP-3 protein 
      production was also examined in vivo. The results were as follows: (1) prunetin 
      inhibited the gene expression of MMP-3, MMP-1, MMP-13, ADAMTS-4, and ADAMTS-5; 
      (2) prunetin inhibited the secretion and proteolytic activity of MMP-3; (3) 
      prunetin suppressed the production of MMP-3 protein in vivo. These results 
      suggest that prunetin can regulate the gene expression, secretion, and 
      proteolytic activity of MMP-3, by directly acting on articular chondrocytes.
FAU - Nam, Dae Cheol
AU  - Nam DC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Kim, Bo Kun
AU  - Kim BK
AD  - Department of Orthopedic Surgery, School of Medicine, Chungnam National 
      University, Daejeon 35015, Korea.
FAU - Lee, Hyun Jae
AU  - Lee HJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 35015, Korea.
FAU - Shin, Hyun-Dae
AU  - Shin HD
AD  - Department of Orthopedic Surgery, School of Medicine, Chungnam National 
      University, Daejeon 35015, Korea.
FAU - Lee, Choong Jae
AU  - Lee CJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 35015, Korea.
FAU - Hwang, Sun-Chul
AU  - Hwang SC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
LA  - eng
PT  - Journal Article
DEP - 20160223
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
PMC - PMC4770113
OTO - NOTNLM
OT  - Chondrocyte
OT  - Metalloproteinase
OT  - Osteoarthritis
OT  - Prunetin
COIS- CONFLICTS OF INTEREST: The authors have no conflicts of interest to declare.
EDAT- 2016/03/05 06:00
MHDA- 2016/03/05 06:01
PMCR- 2016/03/01
CRDT- 2016/03/04 06:00
PHST- 2016/01/15 00:00 [received]
PHST- 2016/01/28 00:00 [revised]
PHST- 2016/02/04 00:00 [accepted]
PHST- 2016/03/04 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2016/03/05 06:01 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - 10.4196/kjpp.2016.20.2.221 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2016 Mar;20(2):221-8. doi: 
      10.4196/kjpp.2016.20.2.221. Epub 2016 Feb 23.