PMID- 26937233
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160303
LR  - 20200929
IS  - 1598-2629 (Print)
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Linking)
VI  - 16
IP  - 1
DP  - 2016 Feb
TI  - TCF4-Targeting miR-124 is Differentially Expressed amongst Dendritic Cell 
      Subsets.
PG  - 61-74
LID - 10.4110/in.2016.16.1.61 [doi]
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that sample their 
      environment and present antigens to naive T lymphocytes for the subsequent 
      antigen-specific immune responses. DCs exist in a range of distinct 
      subpopulations including plasmacytoid DCs (pDCs) and classical DCs (cDCs), with 
      the latter consisting of the cDC1 and cDC2 lineages. Although the roles of 
      DC-specific transcription factors across the DC subsets have become understood, 
      the posttranscriptional mechanisms that regulate DC development are yet to be 
      elucidated. MicroRNAs (miRNAs) are pivotal posttranscriptional regulators of gene 
      expression in a myriad of biological processes, but their contribution to the 
      immune system is just beginning to surface. In this study, our in-house probe 
      collection was screened to identify miRNAs possibly involved in DC development 
      and function by targeting the transcripts of relevant mouse transcription 
      factors. Examination of DC subsets from the culture of mouse bone marrow with 
      Flt3 ligand identified high expression of miR-124 which was able to target the 
      transcript of TCF4, a transcription factor critical for the development and 
      homeostasis of pDCs. Further expression profiling of mouse DC subsets isolated 
      from in vitro culture as well as via ex vivo purification demonstrated that 
      miR-124 was outstandingly expressed in CD24(+) cDC1 cells compared to in pDCs and 
      CD172alpha(+) cDC2 cells. These results imply that miR-124 is likely involved in the 
      processes of DC subset development by posttranscriptional regulation of a 
      transcription factor(s).
FAU - Han, Sun Murray
AU  - Han SM
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul 03722, Korea.; Brain Korea 21 PLUS Project 
      for Medical Science, Severance Biomedical Science Institute, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
FAU - Na, Hye Young
AU  - Na HY
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul 03722, Korea.
FAU - Ham, Onju
AU  - Ham O
AD  - Brain Korea 21 PLUS Project for Medical Science, Severance Biomedical Science 
      Institute, Yonsei University College of Medicine, Seoul 03722, Korea.; Institute 
      for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, 
      Gangneung 25601, Korea.
FAU - Choi, Wanho
AU  - Choi W
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul 03722, Korea.; Brain Korea 21 PLUS Project 
      for Medical Science, Severance Biomedical Science Institute, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
FAU - Sohn, Moah
AU  - Sohn M
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul 03722, Korea.; Brain Korea 21 PLUS Project 
      for Medical Science, Severance Biomedical Science Institute, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
FAU - Ryu, Seul Hye
AU  - Ryu SH
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul 03722, Korea.; Brain Korea 21 PLUS Project 
      for Medical Science, Severance Biomedical Science Institute, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
FAU - In, Hyunju
AU  - In H
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul 03722, Korea.; Brain Korea 21 PLUS Project 
      for Medical Science, Severance Biomedical Science Institute, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
FAU - Hwang, Ki-Chul
AU  - Hwang KC
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung 25601, Korea.
FAU - Park, Chae Gyu
AU  - Park CG
AD  - Laboratory of Immunology, Severance Biomedical Science Institute, Yonsei 
      University College of Medicine, Seoul 03722, Korea.; Brain Korea 21 PLUS Project 
      for Medical Science, Severance Biomedical Science Institute, Yonsei University 
      College of Medicine, Seoul 03722, Korea.
LA  - eng
PT  - Journal Article
DEP - 20160225
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC4770101
OTO - NOTNLM
OT  - Dendritic cells
OT  - MicroRNAs
OT  - Posttranscriptional Gene Silencing
OT  - TCF4
COIS- CONFLICTS OF INTEREST: The authors have no financial conflict of interest.
EDAT- 2016/03/05 06:00
MHDA- 2016/03/05 06:01
PMCR- 2016/02/01
CRDT- 2016/03/04 06:00
PHST- 2015/11/10 00:00 [received]
PHST- 2016/01/08 00:00 [revised]
PHST- 2016/01/15 00:00 [accepted]
PHST- 2016/03/04 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2016/03/05 06:01 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - 10.4110/in.2016.16.1.61 [doi]
PST - ppublish
SO  - Immune Netw. 2016 Feb;16(1):61-74. doi: 10.4110/in.2016.16.1.61. Epub 2016 Feb 
      25.