PMID- 26938744
OWN - NLM
STAT- MEDLINE
DCOM- 20180529
LR  - 20181202
IS  - 1520-5827 (Electronic)
IS  - 0743-7463 (Linking)
VI  - 32
IP  - 19
DP  - 2016 May 17
TI  - Microfluidic Directed Synthesis of Alginate Nanogels with Tunable Pore Size for 
      Efficient Protein Delivery.
PG  - 4996-5003
LID - 10.1021/acs.langmuir.5b04645 [doi]
AB  - Alginate is a biopolymer with favorable pH-sensitive properties for oral delivery 
      of peptides and proteins. However, conventional alginate nanogels have 
      limitations such as low encapsulation efficiency because of drug leaching during 
      bead preparation and burst release in high pH values. These shortcomings 
      originate from large pore size of the nanogels. In this work, we proposed an 
      on-chip hydrodynamic flow focusing approach for synthesis of alginate nanogels 
      with adjustable pore size to achieve fine-tunable release profile of the 
      encapsulated bioactive agents. It is demonstrated that the microstructure of 
      nanogels can be controlled through adjusting flow ratio and mixing time directed 
      on microfluidic platforms consisting of cross-junction microchannels. In this 
      study, the average pore size of alginate nanogels (i.e., average molecular weight 
      between cross-links, Mc) was related to synthesis parameters. Mc was calculated 
      from equations based on equilibrium swelling theory and proposed methods to 
      modify the theory for pH-sensitive nanogels. In the equations we derived, size 
      and compactness of nanogels are key factors, which can be adjusted by controlling 
      the flow ratio. It was found that increase in flow ratio increases the size of 
      nanogels and decreases their compactness. The size of on-chip generated nanogels 
      for flow ratio of 0.02-0.2 was measured to be in the range of 68-138 nm. 
      Moreover, a method based on the Mie theory was implemented to estimate the 
      aggregation number (Nagg) of polymer chains inside the nanogels as an indicator 
      of compactness. According to the size and compactness results along with 
      equations of modified swelling theory, Mc obtained to be in the range of 0.5-0.8 
      kDa. The proposed method could be considered as a promising approach for 
      efficient polypeptides encapsulation and their sustained release.
FAU - Bazban-Shotorbani, Salime
AU  - Bazban-Shotorbani S
AD  - Department of Biomedical Engineering, Amirkabir University of Technology , 
      Tehran, Iran.
FAU - Dashtimoghadam, Erfan
AU  - Dashtimoghadam E
AD  - Department of Developmental Sciences, Marquette University School of Dentistry , 
      Milwaukee, Wisconsin 53201, United States.
FAU - Karkhaneh, Akbar
AU  - Karkhaneh A
AD  - Department of Biomedical Engineering, Amirkabir University of Technology , 
      Tehran, Iran.
FAU - Hasani-Sadrabadi, Mohammad Mahdi
AU  - Hasani-Sadrabadi MM
AD  - Department of Biomedical Engineering, Amirkabir University of Technology , 
      Tehran, Iran.
FAU - Jacob, Karl I
AU  - Jacob KI
LA  - eng
PT  - Journal Article
DEP - 20160502
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Alginates)
RN  - 0 (Drug Carriers)
RN  - 0 (Gels)
RN  - 0 (Hexuronic Acids)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 9002-98-6 (Polyethyleneimine)
SB  - IM
MH  - Alginates/*chemistry
MH  - Animals
MH  - Cattle
MH  - Chemistry Techniques, Synthetic/*instrumentation
MH  - Drug Carriers/*chemical synthesis/*chemistry
MH  - Drug Liberation
MH  - Gels
MH  - Glucuronic Acid/chemistry
MH  - Hexuronic Acids/chemistry
MH  - Hydrodynamics
MH  - Hydrogen-Ion Concentration
MH  - *Lab-On-A-Chip Devices
MH  - Molecular Weight
MH  - Nanostructures/*chemistry
MH  - Polyethyleneimine/chemistry
MH  - Serum Albumin, Bovine/*chemistry
EDAT- 2016/03/05 06:00
MHDA- 2018/05/31 06:00
CRDT- 2016/03/04 06:00
PHST- 2016/03/04 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2018/05/31 06:00 [medline]
AID - 10.1021/acs.langmuir.5b04645 [doi]
PST - ppublish
SO  - Langmuir. 2016 May 17;32(19):4996-5003. doi: 10.1021/acs.langmuir.5b04645. Epub 
      2016 May 2.