PMID- 26939370
OWN - NLM
STAT- MEDLINE
DCOM- 20160504
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 11
DP  - 2015 Nov
TI  - [Determination of docosahexaenoic acid in milk powder by gas chromatography using 
      acid hydrolysis].
PG  - 1214-8
AB  - A method to determine docosahexenoic acid (DHA) in milk powder by gas 
      chromatography was established. The milk powder samples were hydrolyzed with 
      hydrochloric acid, extracted to get total fatty acids by Soxhlet extractor, then 
      esterified with potassium hydroxide methanol solution to form methyl esters, and 
      treated with sodium hydrogen sulfate. The optimal experiment conditions were 
      obtained from orthogonal experiment L9(3(3)) which performed with three factors 
      and three levels, and it requires the reaction performed with 1 mol/L potassium 
      hydroxide solution at 25 degrees C for 5 min. The derivative treated with sodium 
      hydrogen sulfate was separated on a column of SP-2560 (100 m x 0.25 mm x 0.20 
      mum), and determined in 55 min by temperature programming-gas chromatography. Good 
      linearity was obtained in the range 5.0-300 mg/L with the correlation coefficient 
      of 0.999 9. The relative standard deviations (RSDs) were 3.4%, 1.2% and 1.1% for 
      the seven repeated experiments of 10, 50 and 100 mg/L of DHA, respectively. The 
      limit of detection was 2 mg/kg, and the recoveries of DHA were in the range of 
      90.4%-93.5%. The results are satisfactory through the tests of practical samples.
FAU - Shao, Shiping
AU  - Shao S
FAU - Xiang, Dapeng
AU  - Xiang D
FAU - Li, Shuang
AU  - Li S
FAU - Xi, Xinglin
AU  - Xi X
FAU - Chen, Wenrui
AU  - Chen W
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Powders)
RN  - 25167-62-8 (Docosahexaenoic Acids)
SB  - IM
MH  - Animals
MH  - Docosahexaenoic Acids/*analysis
MH  - Milk/*chemistry
MH  - Powders/analysis
EDAT- 2016/03/05 06:00
MHDA- 2016/05/05 06:00
CRDT- 2016/03/05 06:00
PHST- 2016/03/05 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2016/05/05 06:00 [medline]
AID - 10.3724/sp.j.1123.2015.06022 [doi]
PST - ppublish
SO  - Se Pu. 2015 Nov;33(11):1214-8. doi: 10.3724/sp.j.1123.2015.06022.