PMID- 26940016
OWN - NLM
STAT- MEDLINE
DCOM- 20161220
LR  - 20181113
IS  - 1574-4647 (Electronic)
IS  - 0161-9152 (Print)
IS  - 0161-9152 (Linking)
VI  - 38
IP  - 2
DP  - 2016 Apr
TI  - Effects of aerobic training on markers of autophagy in the elderly.
PG  - 33
LID - 10.1007/s11357-016-9897-y [doi]
LID - 33
AB  - Autophagy is a molecular process essential for the maintenance of cellular 
      homeostasis, which appears to (i) decline with age and (ii) respond to physical 
      exercise. In addition, recent evidence suggests a crosstalk between autophagy and 
      toll-like receptor (TLR)-associated inflammatory responses. This study assessed 
      the effects of aerobic exercise training on autophagy and TLR signaling in older 
      subjects. Twenty-nine healthy women and men (age, 69.7 +/- 1.0 year) were 
      randomized to a training (TG) or a control (CG) group. TG performed an 8-week 
      aerobic training program, while CG followed their daily routines. Peripheral 
      blood mononuclear cells were isolated from blood samples obtained before and 
      after the intervention, and protein levels of protein 1 light chain 3 (LC3), 
      sequestosome 1 (p62/SQSTM1), beclin-1, phosphorylated unc-51-like kinase (ULK-1), 
      ubiquitin-like autophagy-related (Atg)12, Atg16, and lysosome-associated membrane 
      protein (LAMP)-2 were measured. TLR2 and TLR4 signaling pathways were also 
      analyzed. Peak oxygen uptake increased in TG after the intervention. Protein 
      expression of beclin-1, Atg12, Atg16, and the LC3II/I ratio increased following 
      the training program (p < 0.05), while expression of p62/SQSTM1 and 
      phosphorylation of ULK-1 at Ser(757) were lower (p < 0.05). Protein content of 
      TLR2, TLR4, myeloid differentiation primary response gen 88 (MyD88), and TIR 
      domain-containing adaptor-inducing interferon (TRIF) were not significantly 
      modified by exercise. The current data indicate that aerobic exercise training 
      induces alterations in multiple markers of autophagy, which seem to be unrelated 
      to changes in TLR2 and TLR4 signaling pathways. These results expand knowledge on 
      exercise-induced autophagy adaptations in humans and suggest that the exercise 
      type employed may be a key factor explaining the potential relationship between 
      autophagy and TLR pathways.
FAU - Mejias-Pena, Yubisay
AU  - Mejias-Pena Y
AD  - Institute of Biomedicine (IBIOMED), University of Leon, Campus Vegazana s/n, 
      24071, Leon, Spain.
FAU - Rodriguez-Miguelez, Paula
AU  - Rodriguez-Miguelez P
AD  - Divisions of Clinical and Translational Sciences, Georgia Prevention Institute, 
      Department of Pediatrics, Augusta University, Augusta, USA.
FAU - Fernandez-Gonzalo, Rodrigo
AU  - Fernandez-Gonzalo R
AD  - Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, 
      Sweden.
FAU - Martinez-Florez, Susana
AU  - Martinez-Florez S
AD  - Institute of Biomedicine (IBIOMED), University of Leon, Campus Vegazana s/n, 
      24071, Leon, Spain.
FAU - Almar, Mar
AU  - Almar M
AD  - Institute of Biomedicine (IBIOMED), University of Leon, Campus Vegazana s/n, 
      24071, Leon, Spain.
FAU - de Paz, Jose A
AU  - de Paz JA
AD  - Institute of Biomedicine (IBIOMED), University of Leon, Campus Vegazana s/n, 
      24071, Leon, Spain.
FAU - Cuevas, Maria J
AU  - Cuevas MJ
AD  - Institute of Biomedicine (IBIOMED), University of Leon, Campus Vegazana s/n, 
      24071, Leon, Spain.
FAU - Gonzalez-Gallego, Javier
AU  - Gonzalez-Gallego J
AD  - Institute of Biomedicine (IBIOMED), University of Leon, Campus Vegazana s/n, 
      24071, Leon, Spain. jgonga@unileon.es.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20160303
PL  - Netherlands
TA  - Age (Dordr)
JT  - Age (Dordrecht, Netherlands)
JID - 101250497
RN  - 0 (Biomarkers)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aging/*metabolism
MH  - Autophagy/*physiology
MH  - Biomarkers/*blood
MH  - Blotting, Western
MH  - Female
MH  - Humans
MH  - Leukocytes, Mononuclear/metabolism
MH  - Male
MH  - Middle Aged
MH  - Physical Fitness/*physiology
MH  - Signal Transduction
MH  - Toll-Like Receptor 4/blood
MH  - Young Adult
PMC - PMC5005904
OTO - NOTNLM
OT  - Autophagy
OT  - Elderly
OT  - High-intensity interval training
OT  - TLR
EDAT- 2016/03/05 06:00
MHDA- 2016/12/21 06:00
PMCR- 2017/04/01
CRDT- 2016/03/05 06:00
PHST- 2015/10/20 00:00 [received]
PHST- 2016/02/23 00:00 [accepted]
PHST- 2016/03/05 06:00 [entrez]
PHST- 2016/03/05 06:00 [pubmed]
PHST- 2016/12/21 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - 10.1007/s11357-016-9897-y [pii]
AID - 9897 [pii]
AID - 10.1007/s11357-016-9897-y [doi]
PST - ppublish
SO  - Age (Dordr). 2016 Apr;38(2):33. doi: 10.1007/s11357-016-9897-y. Epub 2016 Mar 3.