PMID- 26945667
OWN - NLM
STAT- MEDLINE
DCOM- 20170525
LR  - 20181202
IS  - 1365-294X (Electronic)
IS  - 0962-1083 (Print)
IS  - 0962-1083 (Linking)
VI  - 25
IP  - 10
DP  - 2016 May
TI  - Transcriptomic differences between euryhaline and stenohaline malaria vector 
      sibling species in response to salinity stress.
PG  - 2210-25
LID - 10.1111/mec.13609 [doi]
AB  - Evolution of osmoregulatory systems is a key factor in the transition of species 
      between fresh- and saltwater habitats. Anopheles coluzzii and Anopheles merus are 
      stenohaline and euryhaline malaria vector mosquitoes belonging to a larger group 
      of sibling species, the Anopheles gambiae complex, which radiated in Africa 
      within the last 2 million years. Comparative ecological genomics of these vector 
      species can provide insight into the mechanisms that permitted the rapid 
      radiation of this species complex into habitats of contrasting salinity. Here, we 
      use RNA-Seq to investigate gene expression differences between An. coluzzii and 
      An. merus after briefly exposing both young and old larval instars of each 
      species to either saltwater (SW) or freshwater (FW). Our study aims to identify 
      candidate genes and pathways responsible for the greater SW tolerance of 
      An. merus. Our results are congruent with the ability of gene induction to 
      mediate salinity tolerance, with both species showing increasing amounts of 
      differential gene expression between SW and FW as salt concentrations increase. 
      Besides ion transporters such as AgAE2 that may serve as effectors for 
      osmoregulation, we also find mitogen-activated protein kinases that may serve in 
      a phosphorylation signalling pathway responding to salinity, and report potential 
      cross-talk between the mosquito immune response and osmoregulation. This study 
      provides a key step towards applying the growing molecular knowledge of these 
      malaria vectors to improve understanding of their ecological tolerances and 
      habitat occupancy.
CI  - (c) 2016 John Wiley & Sons Ltd.
FAU - Uyhelji, Hilary A
AU  - Uyhelji HA
AD  - Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 
      46556, USA.
AD  - Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, 46556, 
      USA.
AD  - Functional Genomics Team, Civil Aerospace Medical Institute, Federal Aviation 
      Administration, Oklahoma City, OK, 73169, USA.
FAU - Cheng, Changde
AU  - Cheng C
AD  - Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 
      46556, USA.
AD  - Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, 46556, 
      USA.
FAU - Besansky, Nora J
AU  - Besansky NJ
AD  - Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, 
      46556, USA.
AD  - Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, 46556, 
      USA.
LA  - eng
GR  - R21 AI101459/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20160331
PL  - England
TA  - Mol Ecol
JT  - Molecular ecology
JID - 9214478
SB  - IM
MH  - Africa
MH  - Animals
MH  - Anopheles/*genetics/physiology
MH  - Ecosystem
MH  - Fresh Water
MH  - Insect Vectors/genetics/physiology
MH  - Larva/genetics/physiology
MH  - *Salinity
MH  - *Salt Tolerance
MH  - *Transcriptome
PMC - PMC4877251
MID - NIHMS766799
OTO - NOTNLM
OT  - Anopheles merus
OT  - c-Jun N-terminal kinase
OT  - gene expression reaction norm
OT  - osmoregulation
OT  - saltwater tolerance
OT  - transporters
EDAT- 2016/03/08 06:00
MHDA- 2017/05/26 06:00
PMCR- 2017/05/01
CRDT- 2016/03/07 06:00
PHST- 2014/07/07 00:00 [received]
PHST- 2016/01/09 00:00 [revised]
PHST- 2016/02/22 00:00 [accepted]
PHST- 2016/03/07 06:00 [entrez]
PHST- 2016/03/08 06:00 [pubmed]
PHST- 2017/05/26 06:00 [medline]
PHST- 2017/05/01 00:00 [pmc-release]
AID - 10.1111/mec.13609 [doi]
PST - ppublish
SO  - Mol Ecol. 2016 May;25(10):2210-25. doi: 10.1111/mec.13609. Epub 2016 Mar 31.