PMID- 26945849
OWN - NLM
STAT- MEDLINE
DCOM- 20171128
LR  - 20171218
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Linking)
VI  - 217
DP  - 2016 Jun 2
TI  - Experimental infection of European flat oyster Ostrea edulis with ostreid 
      herpesvirus 1 microvar (OsHV-1muvar): Mortality, viral load and detection of viral 
      transcripts by in situ hybridization.
PG  - 55-62
LID - S0168-1702(16)30073-9 [pii]
LID - 10.1016/j.virusres.2016.01.023 [doi]
AB  - Ostreid herpesvirus 1 (OsHV-1) infections have been reported in several bivalve 
      species. Mortality of Pacific oyster Crassostrea gigas spat has increased 
      considerably in Europe since 2008 linked to the spread of a variant of OsHV-1 
      called muvar. In the present study we demonstrated that O. edulis juveniles can be 
      infected by OsHV-1muvar when administered as an intramuscular injection. Mortality 
      in the oysters injected with OsHV-1muvar was first detected 4 days after injection 
      and reached 25% mortality at day 10. Moreover, the high viral load observed and 
      the detection of viral transcripts by in situ hybridization in several tissues of 
      dying oysters suggested that OsHV-1muvar was the cause of mortality in the O. 
      edulis juveniles. This is therefore the first study to provide evidence about the 
      pathogenicity of OsHV-1muvar in a species that does not belong to the Crassostrea 
      genus. Additionally, we present a novel method to detect OsHV-1 transcripts in 
      infected individuals' using in situ hybridization.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Lopez Sanmartin, Monserrat
AU  - Lopez Sanmartin M
AD  - Instituto de Investigacion y Formacion Agraria y Pesquera (IFAPA), Centro Agua 
      del Pino, Junta de Andalucia, Ctra. El Rompido-Punta Umbria, km 4, 21459 Cartaya, 
      Spain. Electronic address: monserratls@yahoo.es.
FAU - Power, Deborah M
AU  - Power DM
AD  - Centro de Ciencias do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 
      8005-139 Faro, Portugal.
FAU - de la Herran, Roberto
AU  - de la Herran R
AD  - Departamento de Genetica de la Universidad de Granada, Spain.
FAU - Navas, Jose I
AU  - Navas JI
AD  - Instituto de Investigacion y Formacion Agraria y Pesquera (IFAPA), Centro Agua 
      del Pino, Junta de Andalucia, Ctra. El Rompido-Punta Umbria, km 4, 21459 Cartaya, 
      Spain.
FAU - Batista, Frederico M
AU  - Batista FM
AD  - Centro de Ciencias do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 
      8005-139 Faro, Portugal; Instituto Portugues do Mar e da Atmosfera, Divisao de 
      Aquicultura e Valorizacao, Estacao Experimental de Moluscicultura de Tavira, Av. 
      5 de Outubro, 8700-305 Olhao, Portugal.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160303
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Viral
MH  - Herpesviridae/*pathogenicity/ultrastructure
MH  - In Situ Hybridization
MH  - Ostrea/*virology
MH  - RNA, Viral/analysis
MH  - Transcription, Genetic
MH  - Viral Load
OTO - NOTNLM
OT  - ISH
OT  - Microvar
OT  - OsHV-1
OT  - Ostrea edulis
OT  - Ostreid herpesvirus 1
OT  - RNA
EDAT- 2016/03/08 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/03/07 06:00
PHST- 2016/01/28 00:00 [received]
PHST- 2016/01/29 00:00 [accepted]
PHST- 2016/03/07 06:00 [entrez]
PHST- 2016/03/08 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - S0168-1702(16)30073-9 [pii]
AID - 10.1016/j.virusres.2016.01.023 [doi]
PST - ppublish
SO  - Virus Res. 2016 Jun 2;217:55-62. doi: 10.1016/j.virusres.2016.01.023. Epub 2016 
      Mar 3.