PMID- 26946533
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20220321
IS  - 1535-3699 (Electronic)
IS  - 1535-3702 (Print)
IS  - 1535-3699 (Linking)
VI  - 241
IP  - 8
DP  - 2016 Apr
TI  - Resveratrol attenuated TNF-alpha-induced MMP-3 expression in human nucleus pulposus 
      cells by activating autophagy via AMPK/SIRT1 signaling pathway.
PG  - 848-53
LID - 10.1177/1535370216637940 [doi]
AB  - Resveratrol (RSV) is known to play a role of anti-TNF-alpha in a number of cell 
      types. However, whether RSV modulates the effects of TNF-alpha on human nucleus 
      pulposus (NP) cells is unknown. The purpose of this study is to investigate 
      whether RSV regulates TNF-alpha-induced matrix metalloproteinase-3 (MMP-3) 
      expression. Via quantitative real-time polymerase chain reaction (qRT-PCR) 
      analysis, we found that MMP-3 expression induced by TNF-alpha was inhibited by RSV 
      treatment. Depending on Western blot and qRT-PCR assay, we found that RSV induced 
      autophagy in human NP cells, whereas inhibition of autophagy remarkably abolished 
      the restraining role of RSV in the TNF-alpha-mediated up-regulation of MMP-3. 
      Furthermore, RSV increased SIRT1 expression and SIRT1 knockdown significantly 
      suppressed RSV-induced autophagy in NP cells. RSV also activated AMP-activated 
      protein kinase (AMPK), while inhibition of AMPK notably abolished RSV-induced 
      SIRT1 expression. Our data showed that RSV attenuated TNF-alpha-induced MMP-3 
      expression in human NP cells by activating autophagy via AMPK/SIRT1 signaling 
      pathway. This new finding suggested that RSV might act as a novel preventive and 
      therapeutic role in intervertebral disc degeneration.
CI  - (c) 2016 by the Society for Experimental Biology and Medicine.
FAU - Wang, Xiao-Hu
AU  - Wang XH
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China.
FAU - Zhu, Lei
AU  - Zhu L
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China.
FAU - Hong, Xin
AU  - Hong X
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China.
FAU - Wang, Yun-Tao
AU  - Wang YT
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China.
FAU - Wang, Feng
AU  - Wang F
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China.
FAU - Bao, Jun-Ping
AU  - Bao JP
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China.
FAU - Xie, Xin-Hui
AU  - Xie XH
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China.
FAU - Liu, Lei
AU  - Liu L
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China.
FAU - Wu, Xiao-Tao
AU  - Wu XT
AD  - Spine Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing 
      210009, Jiangsu, China wuxiaotao@medmail.com.cn.
LA  - eng
PT  - Journal Article
DEP - 20160304
PL  - Switzerland
TA  - Exp Biol Med (Maywood)
JT  - Experimental biology and medicine (Maywood, N.J.)
JID - 100973463
RN  - 0 (Stilbenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.5.1.- (SIRT1 protein, human)
RN  - EC 3.5.1.- (Sirtuin 1)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - AMP-Activated Protein Kinases/antagonists & inhibitors/*physiology
MH  - Autophagy/*drug effects/physiology
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Humans
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Nucleus Pulposus/drug effects/*metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Resveratrol
MH  - Signal Transduction/drug effects/*physiology
MH  - Sirtuin 1/antagonists & inhibitors/*physiology
MH  - Stilbenes/*pharmacology
MH  - Tumor Necrosis Factor-alpha/antagonists & inhibitors/*pharmacology
PMC - PMC4950401
OTO - NOTNLM
OT  - AMPK
OT  - MMP-3
OT  - Resveratrol
OT  - SIRT1
OT  - TNF-alpha
OT  - autophagy
OT  - nucleus pulposus cell
EDAT- 2016/03/08 06:00
MHDA- 2017/06/06 06:00
PMCR- 2016/10/01
CRDT- 2016/03/07 06:00
PHST- 2015/12/06 00:00 [received]
PHST- 2016/02/05 00:00 [accepted]
PHST- 2016/03/07 06:00 [entrez]
PHST- 2016/03/08 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
PHST- 2016/10/01 00:00 [pmc-release]
AID - 1535370216637940 [pii]
AID - 10.1177_1535370216637940 [pii]
AID - 10.1177/1535370216637940 [doi]
PST - ppublish
SO  - Exp Biol Med (Maywood). 2016 Apr;241(8):848-53. doi: 10.1177/1535370216637940. 
      Epub 2016 Mar 4.