PMID- 26947613 OWN - NLM STAT- MEDLINE DCOM- 20171221 LR - 20220331 IS - 1532-3102 (Electronic) IS - 0143-4004 (Print) IS - 0143-4004 (Linking) VI - 43 DP - 2016 Jul TI - A longitudinal study of placental perfusion using dynamic contrast enhanced magnetic resonance imaging in murine pregnancy. PG - 90-7 LID - S0143-4004(15)30115-6 [pii] LID - 10.1016/j.placenta.2015.12.019 [doi] AB - INTRODUCTION: To evaluate changes in placental perfusion with advancing gestation in normal murine pregnancy using dynamic contrast enhanced magnetic resonance imaging (DCE-MRI). METHODS: Seven timed-pregnant CD-1 mice underwent DCE-MRI scanning longitudinally on gestational days (GD) 13, 15 and 17. Placentas were segmented into high (HPZ) and low perfusion zones (LPZ) using tissue similarity mapping. Blood perfusion of the respective regions and the whole placenta was quantified using the steepest slope method. The diameter of the maternal central canal (CC) was also measured. RESULTS: An increase in perfusion was observed between GD13 and GD17 in the overall placenta (p = 0.04) and in the HPZ (p = 0.02). Although perfusion in the LPZ showed a slight increasing trend, it was not significant (p = 0.07). Perfusion, in units of ml/min/100 ml, in the overall placenta and the HPZ was respectively 61.2 +/- 31.2 and 106.2 +/- 56.3 at GD13 (n = 19 placentas); 90.3 +/- 43.7 and 139 +/- 55.4 at GD15 (n = 20); and 104.9 +/- 76.1 and 172.2 +/- 85.6 at GD17 (n = 14). The size of the CC increased with advancing gestation (p < 0.05). DISCUSSION: Using longitudinal DCE-MRI, the gestational age-dependent perfusion change in the normal murine placenta and in its regional compartments was quantified. In mid and late gestations, placental constituent regions differ significantly in their perfusion rates. The CC diameter also showed increase with advancing gestation, which may be playing an important role toward the gestational age-dependent increase in placental perfusion. CI - Copyright (c) 2016. Published by Elsevier Ltd. FAU - Yadav, Brijesh Kumar AU - Yadav BK AD - Department of Radiology, Wayne State University School of Medicine, Detroit, MI, USA; Department of Biomedical Engineering, Wayne State University College of Engineering, Detroit, MI, USA. FAU - Neelavalli, Jaladhar AU - Neelavalli J AD - Department of Radiology, Wayne State University School of Medicine, Detroit, MI, USA; Department of Biomedical Engineering, Wayne State University College of Engineering, Detroit, MI, USA. Electronic address: jneelava@med.wayne.edu. FAU - Krishnamurthy, Uday AU - Krishnamurthy U AD - Department of Radiology, Wayne State University School of Medicine, Detroit, MI, USA; Department of Biomedical Engineering, Wayne State University College of Engineering, Detroit, MI, USA. FAU - Szalai, Gabor AU - Szalai G AD - Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA. FAU - Shen, Yimin AU - Shen Y AD - Department of Radiology, Wayne State University School of Medicine, Detroit, MI, USA. FAU - Nayak, Nihar R AU - Nayak NR AD - Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. FAU - Chaiworapongsa, Tinnakorn AU - Chaiworapongsa T AD - Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. FAU - Hernandez-Andrade, Edgar AU - Hernandez-Andrade E AD - Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA. FAU - Than, Nandor Gabor AU - Than NG AD - Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA; Lendulet Reproduction Research Group, Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary. FAU - Haacke, E Mark AU - Haacke EM AD - Department of Radiology, Wayne State University School of Medicine, Detroit, MI, USA; Department of Biomedical Engineering, Wayne State University College of Engineering, Detroit, MI, USA. FAU - Romero, Roberto AU - Romero R AD - Perinatology Research Branch, NICHD/NIH/DHHS, Bethesda, MD, and Detroit, MI, USA; Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA; Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, MI, USA; Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI, USA. LA - eng GR - HHSN275201300006C/HD/NICHD NIH HHS/United States GR - N01 HD023342/HD/NICHD NIH HHS/United States GR - R42 HL112580/HL/NHLBI NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, N.I.H., Intramural PT - Research Support, Non-U.S. Gov't DEP - 20160104 PL - Netherlands TA - Placenta JT - Placenta JID - 8006349 SB - IM MH - Animals MH - Female MH - *Magnetic Resonance Imaging MH - Mice MH - Placenta/blood supply/*diagnostic imaging MH - Placental Circulation/*physiology MH - Pregnancy PMC - PMC5704953 MID - NIHMS911051 OTO - NOTNLM OT - Central canal OT - Contrast enhanced OT - Junctional zone OT - Labyrinth zone OT - Mice EDAT- 2016/03/08 06:00 MHDA- 2017/12/22 06:00 PMCR- 2017/11/28 CRDT- 2016/03/08 06:00 PHST- 2015/06/25 00:00 [received] PHST- 2015/12/18 00:00 [revised] PHST- 2015/12/31 00:00 [accepted] PHST- 2016/03/08 06:00 [entrez] PHST- 2016/03/08 06:00 [pubmed] PHST- 2017/12/22 06:00 [medline] PHST- 2017/11/28 00:00 [pmc-release] AID - S0143-4004(15)30115-6 [pii] AID - 10.1016/j.placenta.2015.12.019 [doi] PST - ppublish SO - Placenta. 2016 Jul;43:90-7. doi: 10.1016/j.placenta.2015.12.019. Epub 2016 Jan 4.