PMID- 26948147
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181202
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 88
IP  - 7
DP  - 2016 Apr 5
TI  - General Bioluminescence Resonance Energy Transfer Homogeneous Immunoassay for 
      Small Molecules Based on Quantum Dots.
PG  - 3512-20
LID - 10.1021/acs.analchem.5b03581 [doi]
AB  - Here, we describe a general bioluminescence resonance energy transfer (BRET) 
      homogeneous immunoassay based on quantum dots (QDs) as the acceptor and Renilla 
      luciferase (Rluc) as the donor (QD-BRET) for the determination of small 
      molecules. The ratio of the donor-acceptor that could produce energy transfer 
      varied in the presence of different concentrations of free enrofloxacin (ENR), an 
      important small molecule in food safety. The calculated Forster distance (R0) was 
      7.86 nm. Under optimized conditions, the half-maximal inhibitory concentration 
      (IC50) for ENR was less than 1 ng/mL and the linear range covered 4 orders of 
      magnitude (0.023 to 25.60 ng/mL). The cross-reactivities (CRs) of seven 
      representative fluoroquinolones (FQs) were similar to the data obtained by an 
      enzyme-linked immunosorbent assay (ELISA). The average intra- and interassay 
      recoveries from spiked milk of were 79.8-118.0%, and the relative standard 
      deviations (RSDs) were less than 10%, meeting the requirement of residue 
      detection, which was a satisfactory result. Furthermore, we compared the 
      influence of different luciferase substrates on the performance of the assay. 
      Considering sensitivity and stability, coelenterazine-h was the most appropriate 
      substrate. The results from this study will enable better-informed decisions on 
      the choice of Rluc substrate for QD-BRET systems. For the future, the QD-BRET 
      immunosensor could easily be extended to other small molecules and thus 
      represents a versatile strategy in food safety, the environment, clinical 
      diagnosis, and other fields.
FAU - Yu, Xuezhi
AU  - Yu X
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
FAU - Wen, Kai
AU  - Wen K
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
FAU - Wang, Zhanhui
AU  - Wang Z
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
FAU - Zhang, Xiya
AU  - Zhang X
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
FAU - Li, Chenglong
AU  - Li C
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
FAU - Zhang, Suxia
AU  - Zhang S
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
AD  - Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of 
      Detection Technology for Animal-Derived Food Safety , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
FAU - Shen, Jianzhong
AU  - Shen J
AD  - Beijing Advanced Innovation Center for Food Nutrition and Human Health, College 
      of Veterinary Medicine, China Agricultural University , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
AD  - Beijing Laboratory for Food Quality and Safety and Beijing Key Laboratory of 
      Detection Technology for Animal-Derived Food Safety , No. 2 Yuanmingyuan West 
      Road, Beijing 100193, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160316
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Fluoroquinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - EC 1.13.12.5 (Luciferases, Renilla)
SB  - IM
MH  - *Bioluminescence Resonance Energy Transfer Techniques
MH  - Enrofloxacin
MH  - Fluoroquinolones/*analysis/metabolism
MH  - *Immunoassay
MH  - Luciferases, Renilla/chemistry/metabolism
MH  - *Quantum Dots
EDAT- 2016/03/08 06:00
MHDA- 2016/12/15 06:00
CRDT- 2016/03/08 06:00
PHST- 2016/03/08 06:00 [entrez]
PHST- 2016/03/08 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1021/acs.analchem.5b03581 [doi]
PST - ppublish
SO  - Anal Chem. 2016 Apr 5;88(7):3512-20. doi: 10.1021/acs.analchem.5b03581. Epub 2016 
      Mar 16.