PMID- 26948660
OWN - NLM
STAT- MEDLINE
DCOM- 20171207
LR  - 20181113
IS  - 1438-2199 (Electronic)
IS  - 0939-4451 (Print)
IS  - 0939-4451 (Linking)
VI  - 48
IP  - 6
DP  - 2016 Jun
TI  - Proteomic profiling identifies the inorganic pyrophosphatase (PPA1) protein as a 
      potential biomarker of metastasis in laryngeal squamous cell carcinoma.
PG  - 1469-76
LID - 10.1007/s00726-016-2201-8 [doi]
AB  - Relapse and metastasis are the main causes of unfavorable outcome in head and 
      neck cancers. Whereas, understanding of the molecular background of these 
      processes is far from being complete. Therefore, in this study we aimed to 
      identify potential biomarker candidates of relapse and metastasis in laryngeal 
      squamous cell carcinoma (LSCC) by combining the 2D electrophoresis based protein 
      screen and immunohistochemical analysis of candidate proteins. We screened three 
      groups of LSCC cell lines derived from primary tumors, recurrent tumors and 
      metastases and identified seven proteins that differed significantly in relative 
      abundance between the analyzed groups. Among the identified proteins were the 
      heat shock proteins HSP60 and HSP70 that were significantly downregulated both in 
      recurrences- and metastases-derived cell lines but not in primary tumor-derived 
      cell lines. Moreover, we identified significant upregulation of the annexin V, 
      calreticulin and the inorganic pyrophosphatase (PPA1) exclusively in the 
      metastases-derived cell lines. As these upregulated proteins could potentially 
      become novel biomarkers of metastasis, we have compared their abundance in 
      primary tumor LSCC N(0) cases, primary tumor LSCC N(+) cases as well as in LSCC 
      metastases N(+). Our results show an intense increase of cytoplasmic PPA1 
      abundance in the N(+) (p = 0.000042) compared to the N(0) group. In summary, we 
      show a group of proteins deregulated in recurrences and metastases of LSCC. 
      Moreover, we suggest the PPA1 protein as a potential new biomarker for metastasis 
      in this cancer.
FAU - Bodnar, Magdalena
AU  - Bodnar M
AD  - Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus 
      Copernicus University in Torun, Bydgoszcz, Poland.
FAU - Luczak, Magdalena
AU  - Luczak M
AD  - Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
AD  - Institute of Chemical Technology and Engineering, Poznan University of 
      Technology, Poznan, Poland.
FAU - Bednarek, Kinga
AU  - Bednarek K
AD  - Department of Cancer Genetics, Institute of Human Genetics, Polish Academy of 
      Sciences, Poznan, Poland.
FAU - Szylberg, Lukasz
AU  - Szylberg L
AD  - Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus 
      Copernicus University in Torun, Bydgoszcz, Poland.
FAU - Marszalek, Andrzej
AU  - Marszalek A
AD  - Department of Clinical Pathomorphology, Collegium Medicum in Bydgoszcz, Nicolaus 
      Copernicus University in Torun, Bydgoszcz, Poland.
AD  - Chair of Oncologic Pathology and Epidemiology, Poznan University of Medical 
      Sciences and Greater Poland Cancer Center, Poznan, Poland.
FAU - Grenman, Reidar
AU  - Grenman R
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, Turku University 
      Hospital and University of Turku, Turku, Finland.
AD  - Department of Medical Biochemistry, Turku University Hospital and University of 
      Turku, Turku, Finland.
FAU - Szyfter, Krzysztof
AU  - Szyfter K
AD  - Department of Cancer Genetics, Institute of Human Genetics, Polish Academy of 
      Sciences, Poznan, Poland.
AD  - Department of Audiology and Phoniatrics, Poznan University of Medical Sciences, 
      Poznan, Poland.
FAU - Jarmuz-Szymczak, Malgorzata
AU  - Jarmuz-Szymczak M
AD  - Department of Cancer Genetics, Institute of Human Genetics, Polish Academy of 
      Sciences, Poznan, Poland.
AD  - Department of Hematology and Bone Marrow Transplantation, Poznan University of 
      Medical Sciences, Poznan, Poland.
FAU - Giefing, Maciej
AU  - Giefing M
AD  - Department of Cancer Genetics, Institute of Human Genetics, Polish Academy of 
      Sciences, Poznan, Poland. giefingm@man.poznan.pl.
AD  - Department of Otolaryngology and Laryngeal Oncology, Poznan University of Medical 
      Sciences, Poznan, Poland. giefingm@man.poznan.pl.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160307
PL  - Austria
TA  - Amino Acids
JT  - Amino acids
JID - 9200312
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Neoplasm Proteins)
RN  - EC 3.6.1.1 (Inorganic Pyrophosphatase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*metabolism
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - Humans
MH  - Inorganic Pyrophosphatase/*metabolism
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/*metabolism
MH  - *Proteomics
PMC - PMC4875942
OTO - NOTNLM
OT  - Biomarker
OT  - LSCC
OT  - Laryngeal cancer
OT  - Metastasis
OT  - PPA1
EDAT- 2016/03/08 06:00
MHDA- 2017/12/08 06:00
PMCR- 2016/03/07
CRDT- 2016/03/08 06:00
PHST- 2015/09/18 00:00 [received]
PHST- 2016/02/12 00:00 [accepted]
PHST- 2016/03/08 06:00 [entrez]
PHST- 2016/03/08 06:00 [pubmed]
PHST- 2017/12/08 06:00 [medline]
PHST- 2016/03/07 00:00 [pmc-release]
AID - 10.1007/s00726-016-2201-8 [pii]
AID - 2201 [pii]
AID - 10.1007/s00726-016-2201-8 [doi]
PST - ppublish
SO  - Amino Acids. 2016 Jun;48(6):1469-76. doi: 10.1007/s00726-016-2201-8. Epub 2016 
      Mar 7.