PMID- 26949105
OWN - NLM
STAT- MEDLINE
DCOM- 20170614
LR  - 20191210
IS  - 1879-1220 (Electronic)
IS  - 0960-0760 (Linking)
VI  - 164
DP  - 2016 Nov
TI  - Comparison of the biological effects of exogenous and endogenous 
      1,25-dihydroxyvitamin D(3) on the mature osteoblast cell line MLO-A5.
PG  - 374-378
LID - S0960-0760(16)30050-4 [pii]
LID - 10.1016/j.jsbmb.2016.03.004 [doi]
AB  - Clinical and animal data indicate that serum 25-hydroxyvitamin D(3) (25D) exerts 
      an anabolic effect on bone while serum 1alpha,25-dihydroxyvitamin D(3) (1,25D) 
      stimulates bone mineral loss, although the mechanism responsible for these 
      divergent actions is unknown. Biological effects of 25D on bone cells are 
      dependent on the local conversion to 1,25D by the 25-hydroxyvitamin 
      D-1alpha-hydroxylase enzyme, CYP27B1. Therefore, identification of possible 
      differential activities of locally produced and exogenously supplied 1,25D in 
      bone is likely to be informative for guiding optimal administration of vitamin D 
      supplements for bone health. The mature osteoblastic cell line MLO-A5 expresses 
      both the vitamin D receptor (Vdr) and Cyp27b1, and therefore is a suitable model 
      for comparing the activities of 1,25D arising from these sources. Biologically, 
      exogenous and endogenous sources of 1,25D have similar effects on proliferation, 
      mineralisation and induction of a range of genes by MLO-A5 osteoblasts under 
      osteogenic conditions although endogenous 1,25D levels are markedly lower than 
      exogenous levels. Significant differences of pharmacokinetics and 
      pharmacodynamics of 1,25D are evident between these two sources particularly in 
      terms of modulating gene expression for Cyp24a1 and other genes largely expressed 
      by embedded osteoblasts/osteocytes suggesting that endogenously synthesised 1,25D 
      is more efficiently utilised by the differentiating osteoblast.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Yang, Dongqing
AU  - Yang D
AD  - Bone Cell Biology Group, Centre for Orthopaedic and Trauma Research, Discipline 
      of Orthopaedics and Trauma, University of Adelaide, Adelaide, SA 5005, Australia; 
      Discipline of Medicine, University of Adelaide, Adelaide, SA 5005, Australia. 
      Electronic address: dongqing.yang@adelaide.edu.au.
FAU - Anderson, Paul H
AU  - Anderson PH
AD  - Discipline of Medicine, University of Adelaide, Adelaide, SA 5005, Australia; 
      Musculoskeletal Biology Research, School of Pharmacy and Medical Sciences, 
      University of South Australia, Adelaide, SA 5000, Australia.
FAU - Turner, Andrew G
AU  - Turner AG
AD  - Discipline of Medicine, University of Adelaide, Adelaide, SA 5005, Australia; 
      Musculoskeletal Biology Research, School of Pharmacy and Medical Sciences, 
      University of South Australia, Adelaide, SA 5000, Australia.
FAU - Morris, Howard A
AU  - Morris HA
AD  - Discipline of Medicine, University of Adelaide, Adelaide, SA 5005, Australia; 
      Endocrine Bone Research, Chemical Pathology, SA Pathology, Adelaide, SA 5000, 
      Australia; Musculoskeletal Biology Research, School of Pharmacy and Medical 
      Sciences, University of South Australia, Adelaide, SA 5000, Australia.
FAU - Atkins, Gerald J
AU  - Atkins GJ
AD  - Bone Cell Biology Group, Centre for Orthopaedic and Trauma Research, Discipline 
      of Orthopaedics and Trauma, University of Adelaide, Adelaide, SA 5005, Australia.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20160304
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (Connexin 43)
RN  - 0 (GJA1 protein, mouse)
RN  - 0 (Receptors, Calcitriol)
RN  - 1406-16-2 (Vitamin D)
RN  - A288AR3C9H (25-hydroxyvitamin D)
RN  - EC 1.14.15.16 (Cyp24a1 protein, mouse)
RN  - EC 1.14.15.16 (Vitamin D3 24-Hydroxylase)
RN  - EC 1.14.15.18 (25-Hydroxyvitamin D3 1-alpha-Hydroxylase)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.- (Mmp13 protein, mouse)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - 25-Hydroxyvitamin D3 1-alpha-Hydroxylase/*genetics/metabolism
MH  - Animals
MH  - Biotransformation
MH  - Calcitriol/metabolism/*pharmacology
MH  - Carbonic Anhydrases/genetics/metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Connexin 43/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Matrix Metalloproteinase 13/genetics/metabolism
MH  - Mice
MH  - Osteoblasts/cytology/*drug effects/metabolism
MH  - Osteocytes/cytology/*drug effects/metabolism
MH  - Receptors, Calcitriol/genetics/metabolism
MH  - Signal Transduction
MH  - Vitamin D/*analogs & derivatives/metabolism/pharmacology
MH  - Vitamin D3 24-Hydroxylase/genetics/metabolism
OTO - NOTNLM
OT  - 25-hydroxyvitamin D(3)
OT  - Cyp27b1
OT  - Endogenous/exogenous 1,25(OH)(2)-vitamin-D3
OT  - MLO-A5
OT  - Mineralisation
OT  - Osteogenic differentiation
EDAT- 2016/03/08 06:00
MHDA- 2017/06/15 06:00
CRDT- 2016/03/08 06:00
PHST- 2015/06/15 00:00 [received]
PHST- 2016/02/29 00:00 [revised]
PHST- 2016/03/01 00:00 [accepted]
PHST- 2016/03/08 06:00 [pubmed]
PHST- 2017/06/15 06:00 [medline]
PHST- 2016/03/08 06:00 [entrez]
AID - S0960-0760(16)30050-4 [pii]
AID - 10.1016/j.jsbmb.2016.03.004 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 2016 Nov;164:374-378. doi: 
      10.1016/j.jsbmb.2016.03.004. Epub 2016 Mar 4.