PMID- 26950120
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 17
IP  - 3
DP  - 2016 Mar 3
TI  - Biomimetic Membranes for Multi-Redox Center Proteins.
PG  - 330
LID - 10.3390/ijms17030330 [doi]
LID - 330
AB  - His-tag technology was applied for biosensing purposes involving multi-redox 
      center proteins (MRPs). An overview is presented on various surfaces ranging from 
      flat to spherical and modified with linker molecules with nitrile-tri-acetic acid 
      (NTA) terminal groups to bind his-tagged proteins in a strict orientation. The 
      bound proteins are submitted to in situ dialysis in the presence of lipid 
      micelles to form a so-called protein-tethered bilayer lipid membrane (ptBLM). 
      MRPs, such as the cytochrome c oxidase (CcO) from R. sphaeroides and P. 
      denitrificans, as well as photosynthetic reactions centers (RCs) from R. 
      sphaeroides, were thus investigated. Electrochemical and surface-sensitive 
      optical techniques, such as surface plasmon resonance, surface plasmon-enhanced 
      fluorescence, surface-enhanced infrared absorption spectroscopy (SEIRAS) and 
      surface-enhanced resonance Raman spectroscopy (SERRS), were employed in the case 
      of the ptBLM structure on flat surfaces. Spherical particles ranging from microm size 
      agarose gel beads to nm size nanoparticles modified in a similar fashion were 
      called proteo-lipobeads (PLBs). The particles were investigated by laser-scanning 
      confocal fluorescence microscopy (LSM) and UV/Vis spectroscopy. Electron and 
      proton transfer through the proteins were demonstrated to take place, which was 
      strongly affected by the membrane potential. MRPs can thus be used for biosensing 
      purposes under quasi-physiological conditions.
FAU - Naumann, Renate L C
AU  - Naumann RL
AD  - Austrian Institute of Technology GmbH, AIT, Donau-City-Str. 1, 1220 Vienna, 
      Austria. kontakt@rlc-naumann.eu.
FAU - Geiss, Andreas F
AU  - Geiss AF
AD  - Austrian Institute of Technology GmbH, AIT, Donau-City-Str. 1, 1220 Vienna, 
      Austria. andreas.geiss.fl@ait.ac.at.
FAU - Steininger, Christoph
AU  - Steininger C
AD  - Austrian Institute of Technology GmbH, AIT, Donau-City-Str. 1, 1220 Vienna, 
      Austria. christoph.steininger.fl@ait.ac.at.
FAU - Knoll, Wolfgang
AU  - Knoll W
AD  - Austrian Institute of Technology GmbH, AIT, Donau-City-Str. 1, 1220 Vienna, 
      Austria. Wolfgang.Knoll@ait.ac.at.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20160303
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Bacterial Proteins)
RN  - 0 (Immobilized Proteins)
RN  - 0 (Lipid Bilayers)
RN  - 0 (Photosynthetic Reaction Center Complex Proteins)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Bacterial Proteins/*chemistry
MH  - Biomimetics/methods
MH  - Biosensing Techniques
MH  - Electrochemical Techniques
MH  - Electron Transport Complex IV/*chemistry
MH  - Immobilized Proteins/*chemistry
MH  - Lipid Bilayers/*chemistry
MH  - Oxidation-Reduction
MH  - Paracoccus denitrificans/*chemistry
MH  - Photosynthetic Reaction Center Complex Proteins/*chemistry
MH  - Rhodobacter sphaeroides/*chemistry
MH  - Spectrophotometry, Infrared
MH  - Spectrum Analysis, Raman
MH  - Surface Plasmon Resonance
PMC - PMC4813192
OTO - NOTNLM
OT  - UV/Vis spectroscopy
OT  - cytochrome c oxidase
OT  - his-tag technology
OT  - laser-scanning confocal microscopy
OT  - multi-redox center proteins
OT  - photosynthetic reaction centers
OT  - protein-tethered bilayer lipid membrane
OT  - proteo-lipobeads
OT  - surface-enhanced infrared absorption spectroscopy
OT  - surface-enhanced resonance Raman spectroscopy
EDAT- 2016/03/08 06:00
MHDA- 2016/12/15 06:00
PMCR- 2016/03/01
CRDT- 2016/03/08 06:00
PHST- 2015/09/07 00:00 [received]
PHST- 2015/11/27 00:00 [revised]
PHST- 2016/02/17 00:00 [accepted]
PHST- 2016/03/08 06:00 [entrez]
PHST- 2016/03/08 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2016/03/01 00:00 [pmc-release]
AID - ijms17030330 [pii]
AID - ijms-17-00330 [pii]
AID - 10.3390/ijms17030330 [doi]
PST - epublish
SO  - Int J Mol Sci. 2016 Mar 3;17(3):330. doi: 10.3390/ijms17030330.